14335_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: 14335_ARATH
• UniProt AC: P42645
• Protein Name: 14-3-3-like protein GF14 upsilon
• Gene Name: GRF5
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 268
• Subcellular Localization: Cytoplasm . Nucleus . Not associated with microtubules (PubMed:21558460). Translocates from the cytosol to the nucleus when phosphorylated (By similarity).
• Protein Description: Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes. May be involved in cell cycle regulation by binding to soluble EDE1 and sequestering it in an inactive form during the early stages of mitosis..
• Protein Sequence: MSSDSSREENVYLAKLAEQAERYEEMVEFMEKVAKTVETEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEDSRGNSDHVSIIKDYRGKIETELSKICDGILNLLEAHLIPAASLAESKVFYLKMKGDYHRYLAEFKTGAERKEAAESTLVAYKSAQDIALADLAPTHPIRLGLALNFSVFYYEILNSSDRACSLAKQAFDEAISELDTLGEESYKDSTLIMQLLRDNLTLWTSDLNDEAGDDIKEAPKEVQKVDEQAQPPPSQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSSDSSREE ------CCCCCCHHH	42.74	27531888
3	Phosphorylation	-----MSSDSSREEN -----CCCCCCHHHH	39.97	19880383
5	Phosphorylation	---MSSDSSREENVY ---CCCCCCHHHHHH	32.99	27531888
6	Phosphorylation	--MSSDSSREENVYL --CCCCCCHHHHHHH	49.02	27643528
12	Phosphorylation	SSREENVYLAKLAEQ CCHHHHHHHHHHHHH	16.72	27643528
51	Phosphorylation	VEERNLLSVAYKNVI HHHHHHHHHHHHHHH	14.10	25561503
69	Phosphorylation	RASWRIISSIEQKED HHHHHHHHHHHHCCC	23.92	-
192	Phosphorylation	FYYEILNSSDRACSL HHHHHHCCCHHHHHH	30.12	-
209	Phosphorylation	QAFDEAISELDTLGE HHHHHHHHHHHHCCC	38.87	30291188
213	Phosphorylation	EAISELDTLGEESYK HHHHHHHHCCCCHHC	49.65	25368622
218	Phosphorylation	LDTLGEESYKDSTLI HHHCCCCHHCCHHHH	32.93	25368622
219	Phosphorylation	DTLGEESYKDSTLIM HHCCCCHHCCHHHHH	23.30	25368622
222	Phosphorylation	GEESYKDSTLIMQLL CCCHHCCHHHHHHHH	22.44	25368622
223	Phosphorylation	EESYKDSTLIMQLLR CCHHCCHHHHHHHHH	30.36	25368622
234	Phosphorylation	QLLRDNLTLWTSDLN HHHHCCCEEEECCCC	26.89	23776212
237	Phosphorylation	RDNLTLWTSDLNDEA HCCCEEEECCCCCCC	18.57	23776212
238	Phosphorylation	DNLTLWTSDLNDEAG CCCEEEECCCCCCCC	28.63	30291188
267	Phosphorylation	EQAQPPPSQ------ HHCCCCCCC------	55.05	30291188

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of 14335_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of 14335_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of 14335_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GCL1_ARATH	GCL1	physical	22737156
CDPK1_ARATH	CPK1	physical	22737156
EDE1_ARATH	EDE1	physical	21558460

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale Arabidopsis phosphoproteome profiling reveals novelchloroplast kinase substrates and phosphorylation networks.";
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,Grossmann J., Gruissem W., Baginsky S.;
Plant Physiol. 150:889-903(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267, AND MASSSPECTROMETRY.
PubMed: 19376835