XPB1_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: XPB1_ARATH
• UniProt AC: Q38861
• Protein Name: General transcription and DNA repair factor IIH helicase subunit XPB1
• Gene Name: XPB1
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 767
• Subcellular Localization: Nucleus .
• Protein Description: ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription (By similarity). Required during the early stages of development, including seed germination. [PubMed: 11737776]
• Protein Sequence: MGNGERGRPNKKMKYGGKDDQKMKNIQNAEDYYDDADEDSRDGEGEEKKRDFTKLELKPDHGNRPLWACADGRIFLETFSPLYKQAYDFLIAIAEPVCRPESMHEYNLTPHSLYAAVSVGLETETIISVLNKLSKTKLPKEMIEFIHASTANYGKVKLVLKKNRYFIESPFPEVLKRLLSDDVINRARFSSEPYYGGDGFSVGRTCGELEAGPGELLNEAEFAAAAEEKETHSFEIDPAQVENVKQRCLPNALNYPMLEEYDFRNDNVNPDLDMELKPHAQPRPYQEKSLSKMFGNGRARSGIIVLPCGAGKSLVGVSAAARIKKSCLCLATNAVSVDQWAFQFKLWSTIRDDQICRFTSDSKERFRGNAGVVVTTYNMVAFGGKRSEESEKIIEEMRNREWGLLLMDEVHVVPAHMFRKVISITKSHCKLGLTATLVREDERITDLNFLIGPKLYEANWLDLVKGGFIANVQCAEVWCPMTKEFFAEYLKKENSKKKQALYVMNPNKFRACEFLIRFHEQQRGDKIIVFADNLFALTEYAMKLRKPMIYGATSHIERTKILEAFKTSKDVNTVFLSKVGDNSIDIPEANVIIQISSHAGSRRQEAQRLGRILRAKGKLEDRMAGGKEEYNAFFYSLVSTDTQEMYYSTKRQQFLIDQGYSFKVITSLPPPDAGSSLSYHSQEEQLSLLGKVMNAGDDLVGLEQLEEDTDGMALQKARRSMGSMSVMSGSKGMVYMEYNSGRHKSGQQFKKPKDPTKRHNLFKKRYV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
40	Phosphorylation	YDDADEDSRDGEGEE CCCCCCCCCCCCCCH	30.17	30407730
191	Phosphorylation	INRARFSSEPYYGGD HHHHHCCCCCCCCCC	39.62	19880383
194	Phosphorylation	ARFSSEPYYGGDGFS HHCCCCCCCCCCCCC	15.64	23111157

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of XPB1_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of XPB1_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of XPB1_ARATH !!

Protein-Protein Interaction

Oops, there are no PPI records of XPB1_ARATH !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.