WNK3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WNK3_MOUSE
• UniProt AC: Q80XP9
• Protein Name: Serine/threonine-protein kinase WNK3 {ECO:0000305}
• Gene Name: Wnk3 {ECO:0000312|MGI:MGI:2652875}
• Organism: Mus musculus (Mouse).
• Sequence Length: 1757
• Subcellular Localization: Cytoplasm .
• Protein Description: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A1, SLC12A2, SLC12A3, SCNN1A, SCNN1B, SCNN1D and SGK1. Inhibits SLC12A4, SLC12A5, SLC12A6 and SLC12A7. Phosphorylates WNK4. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner..
• Protein Sequence: MATDSGEPASTEDSEKPDGVSFENRAARAVAPLTVEARIKEKYSTFSASGENIERKRFFRKSVEMTEDDKVAESSRRDERKAATNISRVDKVPTNVLRGGQEVKYEQCSKATSESSKDCFKEKTEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESTLKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIKDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLEADTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTLIKKIREKKPAGCLEERRDSQCKYVRNVLPQQQTATLQPTPGPHTAAEYEETEVDQHVRQQFLQGKPQQQSSSVRGDTSSEPTAGPVLHSDTSSHPTVAYSSNQTTSSQEQPKLTQSPVLPVVQGQSSVMPIYAAGVGVVSQSQISPLTIQKVSQIKPVSQPIGAEQQATLQNPDFVRSLNQDVTSVKENTNNPDTPSGNGKQDRNKQRRASCPRPEKGTKFQLTVLQVSVSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLENEKEKFVEELRAIVGQAQEILHVHSAVEKSIGVDSVALESNSNQTGSSEQVLINSASTQTSNESAPQSSPVGRWRFCINQTIKNREAQSPPSLQPSMAMVPGLHPFPSSRNTSNQAISQNTVFTIENNPGHRELFTSKLDHKDVVDGKIGEHASIETEQSSISYQVEDDRQIMTPATDNSNYSAALVCPVPGECEALTSQAGMFMPTYPNQQAAVLADVHIAYPGESVPIGGNAALTSVLVSSDQKPQSLSVQQPTIDAEFISKEGETTVNTETSSPKAVIATQTPGFEPAVILPATILESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSICPESQKDTQSIDSPFSSSAEDILSYSMPEVIAISHCGIQDSPAQSPNFQQTGSKILSNVAASQPAHISVFKKDLNVITSVPSELCLHEMSPDASLPGDPEAYPAAVSSDGTIHLQTGGGYFGLSFTCPSLKNPISRKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEDKRSAIASDPIPLTREFSSDTRALSRCKAMSGSFQRGRFQVITVPQQQPVKMMSFGKDHRPPFNKTTVQSSEQALTFAEAAVSQLIEVEPAMPTHKASVSSRKLRTLYETFKEDKGDPEQGDIVSYSTACETSVSSVATEKNVTSTTEVSVQSGSEPLDKEKNESTPGKQTCTNEFSATLAGNRKSVTKTRPEGDQYLPLREEQAYAQTQNSLFYSPSSPMSSDNESEIEDEDLKVELQRLREKHIQEVVSLQTQQNKELQELYERLRATKDNKAQSSEVPLSPASPRRPRSFKSKLRSRPQSMTHSDNLVVKDALGVESNTVSCQQSPASKKGMFTDDLHKLVDDWTRETVGHFPSKPSLNQLKQSQQKSEAENWNKSCESTPSTMGYTSNWISSLSQIRGAAPTSLPQGLPLPSFHGPLASYGMPHVCQYNAVGAAGYPVQWVGISGPAQQSVVLPTQSGGLFQPGMNLQSFPAPPVQNPASIPPGPK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MATDSGEPAS -----CCCCCCCCCC	42.42	24759943
5	Phosphorylation	---MATDSGEPASTE ---CCCCCCCCCCCC	40.47	25293948
10	Phosphorylation	TDSGEPASTEDSEKP CCCCCCCCCCCCCCC	42.94	25338131
45	Phosphorylation	RIKEKYSTFSASGEN HHHHHHHCCCCCCCC	20.65	28066266
47	Phosphorylation	KEKYSTFSASGENIE HHHHHCCCCCCCCHH	22.93	22322096
49	Phosphorylation	KYSTFSASGENIERK HHHCCCCCCCCHHHH	45.50	28066266
62	Phosphorylation	RKRFFRKSVEMTEDD HHHHHHHHCCCCCCH	21.03	19060867
66	Phosphorylation	FRKSVEMTEDDKVAE HHHHCCCCCCHHHHH	23.82	22807455
74	Phosphorylation	EDDKVAESSRRDERK CCHHHHHHHHHHHHH	21.29	27149854
124	Phosphorylation	KDCFKEKTEKEMEEE HHHHHHHHHHHHHHH	54.07	21454597
140	Phosphorylation	EMKAVATSPSGRFLK HHHHHEECCCCCEEE	13.82	21454597
142	Phosphorylation	KAVATSPSGRFLKFD HHHEECCCCCEEEEE	42.22	20139300
209	Phosphorylation	HPNIVRFYDSWESTL CCCCEEEECCHHHHH	9.86	-
211	Phosphorylation	NIVRFYDSWESTLKG CCEEEECCHHHHHCC	22.74	-
215	Phosphorylation	FYDSWESTLKGKKCI EECCHHHHHCCCEEE	22.45	-
226	Phosphorylation	KKCIVLVTELMTSGT CEEEEEEEECCCCCH	21.17	28576409
231	Phosphorylation	LVTELMTSGTLKTYL EEEECCCCCHHHHHH	18.80	28059163
233	Phosphorylation	TELMTSGTLKTYLKR EECCCCCHHHHHHHH	25.50	28059163
298	Phosphorylation	IGDLGLATLMRTSFA ECHHHHHHHHHHHHH	26.57	26643407
302	Phosphorylation	GLATLMRTSFAKSVI HHHHHHHHHHHHHHC	17.77	26643407
303	Phosphorylation	LATLMRTSFAKSVIG HHHHHHHHHHHHHCC	16.91	26643407
307	Phosphorylation	MRTSFAKSVIGTPEF HHHHHHHHHCCCCHH	18.61	-
390	Phosphorylation	GCIRQNKSERLSIKD HHHHCCCCCCCCHHH	35.18	-
408	Phosphorylation	HAFFAEDTGLRVELA HHHHHCCCCCCEEEE	29.62	-
508	Phosphorylation	CLEERRDSQCKYVRN CCHHHHHHHCHHHHH	35.35	-
648	Phosphorylation	VSQIKPVSQPIGAEQ EHHCCCCCCCCCHHH	17.97	27841257
700	Phosphorylation	RNKQRRASCPRPEKG CCCCHHCCCCCCCCC	4.71	-
852	Phosphorylation	IKNREAQSPPSLQPS HHCCCCCCCCCCCCC	22.37	26643407
855	Phosphorylation	REAQSPPSLQPSMAM CCCCCCCCCCCCCCC	33.34	25293948
859	Phosphorylation	SPPSLQPSMAMVPGL CCCCCCCCCCCCCCC	49.88	26643407
1012	Phosphorylation	SSDQKPQSLSVQQPT CCCCCCCCCCCCCCE	15.35	27841257
1031	Phosphorylation	FISKEGETTVNTETS EECCCCCEEEECCCC	3.22	20415495
1032	Phosphorylation	ISKEGETTVNTETSS ECCCCCEEEECCCCC	16.44	20415495
1035	Phosphorylation	EGETTVNTETSSPKA CCCEEEECCCCCCCE	2.87	20415495
1037	Phosphorylation	ETTVNTETSSPKAVI CEEEECCCCCCCEEE	24.84	20415495
1038	Phosphorylation	TTVNTETSSPKAVIA EEEECCCCCCCEEEE	36.26	29899451
1039	Phosphorylation	TVNTETSSPKAVIAT EEECCCCCCCEEEEE	53.68	25521595
1060	Phosphorylation	PAVILPATILESDGE CEEEEEEEEECCCCC	56.31	29899451
1161	O-linked_Glycosylation	ILSNVAASQPAHISV HHHHHHHCCCCEEEE	2.71	55414615
1412	Phosphorylation	ATEKNVTSTTEVSVQ CCCCCCCCCEEEEEC	61.84	27841257
1485	Phosphorylation	NSLFYSPSSPMSSDN CCCCCCCCCCCCCCC	31.61	-
1544	Phosphorylation	TKDNKAQSSEVPLSP CCCCCCCCCCCCCCC	23.39	27841257
1545	Phosphorylation	KDNKAQSSEVPLSPA CCCCCCCCCCCCCCC	5.75	28066266
1550	Phosphorylation	QSSEVPLSPASPRRP CCCCCCCCCCCCCCC	21.67	26643407
1553	Phosphorylation	EVPLSPASPRRPRSF CCCCCCCCCCCCCCH	37.10	26643407
1559	Phosphorylation	ASPRRPRSFKSKLRS CCCCCCCCHHHHHHC	5.24	-
1566	Phosphorylation	SFKSKLRSRPQSMTH CHHHHHHCCCCCCCC	2.67	26643407
1570	Phosphorylation	KLRSRPQSMTHSDNL HHHCCCCCCCCCCCE	59.32	26643407
1572	Phosphorylation	RSRPQSMTHSDNLVV HCCCCCCCCCCCEEE	43.02	26643407
1574	Phosphorylation	RPQSMTHSDNLVVKD CCCCCCCCCCEEEEC	22.27	26643407
1591	Phosphorylation	GVESNTVSCQQSPAS CCCCCEEECCCCCCC	39.44	28066266
1595	Phosphorylation	NTVSCQQSPASKKGM CEEECCCCCCCCCCC	48.81	28066266
1598	Phosphorylation	SCQQSPASKKGMFTD ECCCCCCCCCCCCCH	59.76	28066266

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of WNK3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WNK3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WNK3_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of WNK3_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.