WNK2_MOUSE - dbPTM
WNK2_MOUSE - PTM Information in dbPTM
Basic Information of Protein
UniProt ID WNK2_MOUSE
UniProt AC Q3UH66
Protein Name Serine/threonine-protein kinase WNK2 {ECO:0000305}
Gene Name Wnk2 {ECO:0000312|MGI:MGI:1922857}
Organism Mus musculus (Mouse).
Sequence Length 2149
Subcellular Localization Cytoplasm . Cell membrane .
Protein Description Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity..
Protein Sequence MDGDGGRRDAPGALMEAGRGTGSAGMAEPRARAARLGPQRFLRRSVVESDQEEPPGLEAAETPSAQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAAPAAPPGSPSVPSDPGPERAGTQEPSPDPTTASAAATQVPDGGPRQEEAPAPTQEDAGTTEAKPEPGRARKDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIDSGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQSQEPKDSGSPDKARGLPAPLQVQVTYHAQSGQPGQPEPEEPEADQHLLPPTLPASVTSLASDSTFDSGQGSTVYSDSQSSQQSMVLSSLVDTAPTPASCVCSPPVSEGPGLTHSLPTLGAFQQPATVPGLSVGPVPPPARPPLLQQHFPESSMSFTPVLPPPSTPVPTGPSQPAPPVQQPLPMAQPPTLPQVLAPQPMGTVQPVPSHLPPYLAPTSQVVAPAQLKPLQMPQPPLQPLAQVPPQMPQMPVVPPITPLTGLDGLPQTLTDLPAANVAPVPPPQYFSPAVILPSLTTPLPTSPALPMQAVKLPHPPGTPLAVPCQTIVPNAPAAIPLLAVAPQGVAALSIHPAVAQIPAQPVYPAAFPQMVPGDIPPSPHHTVQSLRATPPQLASPVPPQPVQPSVIHLPEQAAPTAASGTQVLLGHPPSYTADVAAPVSAVSLPPAVLSPPLPDTLLPTVPDLLPKVPSSLAPTVVAASQSAPAQTSSLLLPTNPPLPTGPAVAGPCPAVQLMVEVAQEEQVSQDKPPGPPQSSESFGGSDVTSGRDLSDSCEGTFGGGRLEGRTARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILPAERETFIEQMKDVMDKAEDMLSEDTDADHGSDTGTSPPHLGTCGLATGEENRQSQANAPVYQQNVLHTGKRWFIICPVAEHPATDTSESSPPLPLSSLQPEASQDPAPYPDQLSLTDKPSFPAAQQLLSQAGSSNPPGGASAPLAPSSPPVTTVIPAAPATSTVPESAAGTAMQAGGPGTHQGPASVHETLQPLAETRSAQCTAQPLSTGQGPCTPALEASRCSTGLGEPISTREVSTQGEPLPASVPEPSPPTGATQSVPGQPPPPLPITVGAISLAAPQLPSPPLGPTAPPPPPSALESDGEGPPPRVGFVDNTIKSLDEKLRTLLYQEHVPTSSASAGTPMEASDRDFTLEPLRGDLPSALSDKTPSLTQQTQPSLEKSETAPAGWALAQREQGASSPMTAESSSSNTLGCDSDAGQVASDSSTAPSVPQDASGSSVPTHMDPKDQNSSVPREALAAPMQSGPGSFTVGSPAQLRGARDSGSPHKRPGQQDNSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEIPSSPEVKLAVRRVQTASSIEVGVEEPASSDSGDERPRRRSQVQKQSSLPGTGGVASDFVKKATAFLHRSSRAGSLGPETPSRAGVKVPTISITSFHSQSSYISSDNDSEFEDADIKKELRSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPMGRRRKTSKSKLKAGKLLNPLVQQLKVVASSTGHLSDSSRGPPTKDPRGTKAVQTQQPCSVRASLSTDICSGLASDGGGARGQGWTVYHPTSERGAYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSSLYDSPGSSTSSLAPGPEPGPQPTLHVQAQVNNSNNKKGTFTDDLHKLVDEWTTKTVGAAQVKPTLNQLKQTQKLHDMEASGDARATSVPRAAVGASCLAPAPGPLSTTATPGATPALPVPIPDPESEKPD
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
19MethylationGALMEAGRGTGSAGM
CHHCCCCCCCCCCCC		46.6924129315
30MethylationSAGMAEPRARAARLG
CCCCCCHHHHHHHHC		28.3624129315
45PhosphorylationPQRFLRRSVVESDQE
HHHHHHHHHHCCCCC		24.8719060867
49PhosphorylationLRRSVVESDQEEPPG
HHHHHHCCCCCCCCC		32.7425521595
107PhosphorylationAPAAPPGSPSVPSDP
CCCCCCCCCCCCCCC		21.5629899451
112PhosphorylationPGSPSVPSDPGPERA
CCCCCCCCCCCCCCC		55.0429899451
280PhosphorylationLVTELMTSGTLKTYL
EEEECCCCCHHHHHH		18.8028059163
282PhosphorylationTELMTSGTLKTYLKR
EECCCCCHHHHHHHH		25.5028059163
352PhosphorylationLATLKRASFAKSVIG
HHHHHHHHHHHHHHC		29.7522942356
356PhosphorylationKRASFAKSVIGTPEF
HHHHHHHHHHCCCCH		18.6122817900
472PhosphorylationEEDHGRKSTIALRLW
CCCCCCCCEEEEEEE		24.56-
473PhosphorylationEDHGRKSTIALRLWV
CCCCCCCEEEEEEEE		17.19-
558PhosphorylationQSQEPKDSGSPDKAR
HCCCCCCCCCCCHHC		47.5029899451
560PhosphorylationQEPKDSGSPDKARGL
CCCCCCCCCCHHCCC		33.6020415495
1098PhosphorylationVTSGRDLSDSCEGTF
CCCCCCCCHHCCCCC		31.5925521595
1100PhosphorylationSGRDLSDSCEGTFGG
CCCCCCHHCCCCCCC		15.6422210690
1104PhosphorylationLSDSCEGTFGGGRLE
CCHHCCCCCCCCCCC		9.2322210690
1210PhosphorylationDKAEDMLSEDTDADH
HHHHHHHCCCCCCCC		27.13-
1213PhosphorylationEDMLSEDTDADHGSD
HHHHCCCCCCCCCCC		29.09-
1219PhosphorylationDTDADHGSDTGTSPP
CCCCCCCCCCCCCCC		28.6922807455
1221PhosphorylationDADHGSDTGTSPPHL
CCCCCCCCCCCCCCC		44.0229899451
1223PhosphorylationDHGSDTGTSPPHLGT
CCCCCCCCCCCCCCC		39.9022807455
1224PhosphorylationHGSDTGTSPPHLGTC
CCCCCCCCCCCCCCC		37.4729899451
1387PhosphorylationQPLAETRSAQCTAQP
HHHHHHCCCEEECCC		30.0630635358
1391PhosphorylationETRSAQCTAQPLSTG
HHCCCEEECCCCCCC		19.0830635358
1396PhosphorylationQCTAQPLSTGQGPCT
EEECCCCCCCCCCCC		36.8630635358
1397PhosphorylationCTAQPLSTGQGPCTP
EECCCCCCCCCCCCH		41.0030635358
1403PhosphorylationSTGQGPCTPALEASR
CCCCCCCCHHHHHHH		18.0930635358
1409PhosphorylationCTPALEASRCSTGLG
CCHHHHHHHCCCCCC		25.1730635358
1507PhosphorylationFVDNTIKSLDEKLRT
CCHHHHHHHHHHHHH		36.88-
1566PhosphorylationLTQQTQPSLEKSETA
CCCCCCCCCCCCCCC		39.0721733846
1570PhosphorylationTQPSLEKSETAPAGW
CCCCCCCCCCCCHHH		30.7029899451
1594PhosphorylationSSPMTAESSSSNTLG
CCCCCCCCCCCCCCC		32.81-
1615PhosphorylationQVASDSSTAPSVPQD
CCCCCCCCCCCCCCC		46.6822807455
1652PhosphorylationALAAPMQSGPGSFTV
HHHCCCCCCCCCEEC		40.4823737553
1656PhosphorylationPMQSGPGSFTVGSPA
CCCCCCCCEECCCHH		22.3723737553
1658PhosphorylationQSGPGSFTVGSPAQL
CCCCCCEECCCHHHH		26.1723737553
1661PhosphorylationPGSFTVGSPAQLRGA
CCCEECCCHHHHCCC		16.8423737553
1673PhosphorylationRGARDSGSPHKRPGQ
CCCCCCCCCCCCCCC		27.9929899451
1684PhosphorylationRPGQQDNSSPAKTVG
CCCCCCCCCCCCEEE		46.0929899451
1685PhosphorylationPGQQDNSSPAKTVGR
CCCCCCCCCCCEEEE		34.8229899451
1698O-linked_GlycosylationGRFSVVSTQDEWTLA
EEEEEEECCCCEEEC		28.5622517741
1706PhosphorylationQDEWTLASPHSLRYS
CCCEEECCCCCCCCC		26.6829899451
1712PhosphorylationASPHSLRYSAPPDVY
CCCCCCCCCCCCCEE		17.9319060867
1713PhosphorylationSPHSLRYSAPPDVYL
CCCCCCCCCCCCEEC		28.0619060867
1725PhosphorylationVYLDEIPSSPEVKLA
EECCCCCCCHHHHHH		65.2723737553
1726PhosphorylationYLDEIPSSPEVKLAV
ECCCCCCCHHHHHHH		21.5230372032
1738PhosphorylationLAVRRVQTASSIEVG
HHHEEEECCCEEEEE		26.16-
1751PhosphorylationVGVEEPASSDSGDER
EECCCCCCCCCCCCC		45.4529899451
1752PhosphorylationGVEEPASSDSGDERP
ECCCCCCCCCCCCCH		37.5929899451
1754PhosphorylationEEPASSDSGDERPRR
CCCCCCCCCCCCHHH		50.5229899451
1769PhosphorylationRSQVQKQSSLPGTGG
HHHHHHHHCCCCCCH		40.6625521595
1770PhosphorylationSQVQKQSSLPGTGGV
HHHHHHHCCCCCCHH		35.7725521595
1774PhosphorylationKQSSLPGTGGVASDF
HHHCCCCCCHHHHHH		28.9023737553
1793PhosphorylationTAFLHRSSRAGSLGP
HHHHHHHCCCCCCCC		26.5529899451
1797PhosphorylationHRSSRAGSLGPETPS
HHHCCCCCCCCCCCC		29.2125177544
1802PhosphorylationAGSLGPETPSRAGVK
CCCCCCCCCCCCCCC		29.6923737553
1822PhosphorylationITSFHSQSSYISSDN
EEEEECCCCCCCCCC		28.2929899451
1826PhosphorylationHSQSSYISSDNDSEF
ECCCCCCCCCCCCCC		24.3329899451
1827PhosphorylationSQSSYISSDNDSEFE
CCCCCCCCCCCCCCC		30.7629899451
1884PhosphorylationPNVGFFHTAPPMGRR
CCCCCCCCCCCCCCC		35.39-
1962PhosphorylationDICSGLASDGGGARG
CCHHHCCCCCCCCCC		41.66-
2058PhosphorylationNSNNKKGTFTDDLHK
CCCCCEEECCHHHHH		32.0421082442
2060PhosphorylationNNKKGTFTDDLHKLV
CCCEEECCHHHHHHH		28.5726643407
2073AcetylationLVDEWTTKTVGAAQV
HHHHHHHCCCCHHHH		33.5619861893
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources

Oops, there are no upstream regulatory protein records of WNK2_MOUSE !!
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of WNK2_MOUSE !!
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of WNK2_MOUSE !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions

Oops, there are no PPI records of WNK2_MOUSE !!
Drug and Disease Associations
Kegg Drug
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of WNK2_MOUSE

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"WNK2 is a novel regulator of essential neuronal cation-chloridecotransporters.";
Rinehart J., Vazquez N., Kahle K.T., Hodson C.A., Ring A.M.,Gulcicek E.E., Louvi A., Bobadilla N.A., Gamba G., Lifton R.P.;
J. Biol. Chem. 286:30171-30180(2011).
Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-1566; SER-1770AND SER-1797, AND INTERACTION WITH STK39.

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