WASP_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WASP_MOUSE
• UniProt AC: P70315
• Protein Name: Wiskott-Aldrich syndrome protein homolog
• Gene Name: Was
• Organism: Mus musculus (Mouse).
• Sequence Length: 520
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria..
• Protein Sequence: MNSGPGPVGGRPGGRGGPAVQQNIPSNLLQDHENQRLFELLGRKCWTLATTVVQLYLALPPGAEHWTMEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYLTPTPFFHTFAGDDCQVGLNFADESEAQAFRALVQEKIQKRNQRQSGERRQLPPPPAPINEERRGGLPPVPPHPGGDHGGPSGGPLSLGLVTVDIQNPDITSSRYRGLPAPGPGPTDKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPCRGGGGGGGGGGGGGGGGGGQPLRPPVVGSNKGRSGPLPPVPMGGAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPPPPPLPGAGGPPAPPPPPPPPPPPPCPGSGPAPPPLPPTPVSGGSPAPGGGRGALLDQIRQGIQLNKTPGALENSVQQPPAQQSEGLVGALMHVMQKRSRVIHSSDEGEDQTGEDEEDDEWDD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MNSGPGPVGG -----CCCCCCCCCC	45.10	22802335
214	Phosphorylation	PDITSSRYRGLPAPG CCCCCCCCCCCCCCC	15.81	25367039
236	Phosphorylation	RSGKKKISKADIGAP CCCCCCCCHHHCCCC	30.58	25266776
279	Phosphorylation	LFSRAGISEAQLTDA HHHHHCCCHHHCCCH	26.43	25367039
284	Phosphorylation	GISEAQLTDAETSKL CCCHHHCCCHHHHHH	22.53	25367039
288	Phosphorylation	AQLTDAETSKLIYDF HHCCCHHHHHHHHHH	32.65	25367039
289	Phosphorylation	QLTDAETSKLIYDFI HCCCHHHHHHHHHHH	19.57	26745281
293	Phosphorylation	AETSKLIYDFIEDQG HHHHHHHHHHHHHCC	18.42	21082442
322	Glutathionylation	LPPPPPPCRGGGGGG CCCCCCCCCCCCCCC	8.55	24333276
351	Phosphorylation	LRPPVVGSNKGRSGP CCCCCCCCCCCCCCC	24.61	24224561
381	Methylation	RGPPPPGRGGPPPPP CCCCCCCCCCCCCCC	53.31	16287791
496	Phosphorylation	MHVMQKRSRVIHSSD HHHHHHCCCEECCCC	37.62	23375375
501	Phosphorylation	KRSRVIHSSDEGEDQ HCCCEECCCCCCCCC	28.49	25521595
502	Phosphorylation	RSRVIHSSDEGEDQT CCCEECCCCCCCCCC	26.06	25521595
509	Phosphorylation	SDEGEDQTGEDEEDD CCCCCCCCCCCCCCC	55.66	23375375

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
293	Y	Phosphorylation	Kinase	FYN	P39688	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WASP_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WASP_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DLG1_MOUSE	Dlg1	physical	15699074
ZAP70_MOUSE	Zap70	physical	15699074
LCK_MOUSE	Lck	physical	15699074

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"The phagosomal proteome in interferon-gamma-activated macrophages.";
Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.;
Immunity 30:143-154(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-293; SER-501 ANDSER-502, AND MASS SPECTROMETRY.
PubMed: 19144319