WAC_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WAC_MOUSE
• UniProt AC: Q924H7
• Protein Name: WW domain-containing adapter protein with coiled-coil
• Gene Name: Wac
• Organism: Mus musculus (Mouse).
• Sequence Length: 646
• Subcellular Localization: Nucleus speckle . Nucleus . In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes.
• Protein Description: Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. Also acts as a negative regulator of basal autophagy. Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation. May negatively regulate the ubiquitin proteasome pathway..
• Protein Sequence: MVMYARKQQRLSDGCHDRRGDSQPFQALKYSSKSHPSSGDHRHEKMRDAADPSPPNKMLRRSNSPENKYSDSTGHNKAKNVHTQRVRERDGGTSYSPQENSHNHSALHSSNSHSSNPSNNPSKTSDAPYDSADDWSEHISSSGKKYYYNCRTEVSQWEKPKEWLEREQRQKEANKLAVNSFPKDRDYRREVMQATATSGFTSGMEDKHSSDASSLLPQNILSQTSRHNDKDYRLPRAETHSSSTPVQHPIKPVVHPTATPSTVPSSPFTLQSDHQPKKSFDANGASTLSKLPTPTASLPAQKTERKESAPGDKSISHSCTTPSTSSASGLNPTSAPPTSASAVPVSPVPQSTIPPLLQDPNLFRQLLPALQATLQLNNSNVDISKINEVLTAAVTQASLQSIIHKFLTAGPSAFNITSLISQAAQLSTQAQPSNQSPMSLTSDASSPRSYVSPRISTPQTNTVPMKPLISTPPVSSQPKVSTPVVKQGPVSHSATQQPVTADKQQSHDPVSPRSLQRLSSQRSPSPGPNHTCSSNASTATVVPQNASARPACSLTPTLAAHFNDNLIKHVQGWPADHAEKQASRLREEAHNMGSVHMSEICTELKNLRSLVRVCEIQATLREQRILFLRQQIKELEKLKNQNSFMV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8 (in isoform 2)	Phosphorylation	-	28.24	25521595
12	Phosphorylation	ARKQQRLSDGCHDRR CCCCHHCCCCCCCCC	33.86	27841257
22	Phosphorylation	CHDRRGDSQPFQALK CCCCCCCCCCHHHHH	42.45	28066266
53	Phosphorylation	MRDAADPSPPNKMLR HHHCCCCCCCCHHHH	54.24	25521595
62	Phosphorylation	PNKMLRRSNSPENKY CCHHHHCCCCCCCCC	34.91	27087446
64	Phosphorylation	KMLRRSNSPENKYSD HHHHCCCCCCCCCCC	34.53	27087446
69	Phosphorylation	SNSPENKYSDSTGHN CCCCCCCCCCCCCCC	29.32	25777480
70	Phosphorylation	NSPENKYSDSTGHNK CCCCCCCCCCCCCCC	27.05	26745281
72	Phosphorylation	PENKYSDSTGHNKAK CCCCCCCCCCCCCCC	30.28	30635358
73	Phosphorylation	ENKYSDSTGHNKAKN CCCCCCCCCCCCCCC	47.66	30635358
93	Phosphorylation	VRERDGGTSYSPQEN CCCCCCCCCCCCCCC	29.98	25777480
94	Phosphorylation	RERDGGTSYSPQENS CCCCCCCCCCCCCCC	27.21	26643407
95	Phosphorylation	ERDGGTSYSPQENSH CCCCCCCCCCCCCCC	24.98	25777480
96	Phosphorylation	RDGGTSYSPQENSHN CCCCCCCCCCCCCCC	21.63	27742792
101	Phosphorylation	SYSPQENSHNHSALH CCCCCCCCCCCCCCC	25.55	25777480
105	Phosphorylation	QENSHNHSALHSSNS CCCCCCCCCCCCCCC	37.53	25777480
109	Phosphorylation	HNHSALHSSNSHSSN CCCCCCCCCCCCCCC	31.87	25777480
110	Phosphorylation	NHSALHSSNSHSSNP CCCCCCCCCCCCCCC	31.44	25777480
112	Phosphorylation	SALHSSNSHSSNPSN CCCCCCCCCCCCCCC	27.05	25777480
114	Phosphorylation	LHSSNSHSSNPSNNP CCCCCCCCCCCCCCC	31.47	25777480
115	Phosphorylation	HSSNSHSSNPSNNPS CCCCCCCCCCCCCCC	47.54	25777480
118	Phosphorylation	NSHSSNPSNNPSKTS CCCCCCCCCCCCCCC	54.19	25777480
122	Phosphorylation	SNPSNNPSKTSDAPY CCCCCCCCCCCCCCC	51.17	25777480
131	Phosphorylation	TSDAPYDSADDWSEH CCCCCCCCCCHHHHH	27.96	25338131
136	Phosphorylation	YDSADDWSEHISSSG CCCCCHHHHHHHHCC	26.71	23684622
142	Phosphorylation	WSEHISSSGKKYYYN HHHHHHHCCCEEEEE	48.09	-
222	Phosphorylation	LLPQNILSQTSRHND HCCHHHHHHHHCCCC	27.84	-
225	Phosphorylation	QNILSQTSRHNDKDY HHHHHHHHCCCCCCC	24.73	-
265	Phosphorylation	ATPSTVPSSPFTLQS CCCCCCCCCCCCCCC	46.43	26643407
266	Phosphorylation	TPSTVPSSPFTLQSD CCCCCCCCCCCCCCC	20.26	26643407
269	Phosphorylation	TVPSSPFTLQSDHQP CCCCCCCCCCCCCCC	27.54	29550500
272	Phosphorylation	SSPFTLQSDHQPKKS CCCCCCCCCCCCCCC	39.92	29550500
279	Phosphorylation	SDHQPKKSFDANGAS CCCCCCCCCCCCCCC	34.19	28973931
293	Phosphorylation	STLSKLPTPTASLPA CHHHCCCCCCCCCCC	43.65	28833060
295	Phosphorylation	LSKLPTPTASLPAQK HHCCCCCCCCCCCCC	31.21	28833060
297	Phosphorylation	KLPTPTASLPAQKTE CCCCCCCCCCCCCCC	37.05	28833060
302	Acetylation	TASLPAQKTERKESA CCCCCCCCCCCCCCC	55.23	23806337
433	Phosphorylation	LSTQAQPSNQSPMSL HHHHCCCCCCCCCCC	35.11	23984901
436	Phosphorylation	QAQPSNQSPMSLTSD HCCCCCCCCCCCCCC	27.16	23984901
439	Phosphorylation	PSNQSPMSLTSDASS CCCCCCCCCCCCCCC	31.70	23984901
441	Phosphorylation	NQSPMSLTSDASSPR CCCCCCCCCCCCCCC	19.77	23984901
442	Phosphorylation	QSPMSLTSDASSPRS CCCCCCCCCCCCCCC	36.15	23984901
445	Phosphorylation	MSLTSDASSPRSYVS CCCCCCCCCCCCCCC	45.96	23984901
446	Phosphorylation	SLTSDASSPRSYVSP CCCCCCCCCCCCCCC	26.50	26824392
449	Phosphorylation	SDASSPRSYVSPRIS CCCCCCCCCCCCCCC	33.02	27717184
452	Phosphorylation	SSPRSYVSPRISTPQ CCCCCCCCCCCCCCC	10.54	23737553
456	Phosphorylation	SYVSPRISTPQTNTV CCCCCCCCCCCCCCC	35.00	21082442
457	Phosphorylation	YVSPRISTPQTNTVP CCCCCCCCCCCCCCC	19.62	26824392
460	Phosphorylation	PRISTPQTNTVPMKP CCCCCCCCCCCCCCC	33.78	26239621
462	Phosphorylation	ISTPQTNTVPMKPLI CCCCCCCCCCCCCCC	29.40	26239621
470	Phosphorylation	VPMKPLISTPPVSSQ CCCCCCCCCCCCCCC	42.41	26239621
471	Phosphorylation	PMKPLISTPPVSSQP CCCCCCCCCCCCCCC	25.31	21082442
475	Phosphorylation	LISTPPVSSQPKVST CCCCCCCCCCCCCCC	29.09	26160508
476	Phosphorylation	ISTPPVSSQPKVSTP CCCCCCCCCCCCCCC	51.22	26160508
482	Phosphorylation	SSQPKVSTPVVKQGP CCCCCCCCCCEECCC	24.08	22817900
491	Phosphorylation	VVKQGPVSHSATQQP CEECCCCCCCCCCCC	18.17	25777480
493	Phosphorylation	KQGPVSHSATQQPVT ECCCCCCCCCCCCCC	26.04	25777480
495	Phosphorylation	GPVSHSATQQPVTAD CCCCCCCCCCCCCCC	30.65	25777480
500	Phosphorylation	SATQQPVTADKQQSH CCCCCCCCCCCHHCC	35.50	25777480
506	Phosphorylation	VTADKQQSHDPVSPR CCCCCHHCCCCCCHH	27.62	23684622
511	Phosphorylation	QQSHDPVSPRSLQRL HHCCCCCCHHHHHHH	21.71	25521595
519	Phosphorylation	PRSLQRLSSQRSPSP HHHHHHHHHCCCCCC	26.98	26160508
520	Phosphorylation	RSLQRLSSQRSPSPG HHHHHHHHCCCCCCC	33.92	26160508
523	Phosphorylation	QRLSSQRSPSPGPNH HHHHHCCCCCCCCCC	23.30	23684622
525	Phosphorylation	LSSQRSPSPGPNHTC HHHCCCCCCCCCCCC	43.67	26824392
531	Phosphorylation	PSPGPNHTCSSNAST CCCCCCCCCCCCCCC	22.26	26160508
533	Phosphorylation	PGPNHTCSSNASTAT CCCCCCCCCCCCCEE	27.99	26160508
534	Phosphorylation	GPNHTCSSNASTATV CCCCCCCCCCCCEEE	38.26	26160508
537	Phosphorylation	HTCSSNASTATVVPQ CCCCCCCCCEEECCC	23.87	26160508
538	Phosphorylation	TCSSNASTATVVPQN CCCCCCCCEEECCCC	24.76	26160508
540	Phosphorylation	SSNASTATVVPQNAS CCCCCCEEECCCCCC	23.33	26160508
547	Phosphorylation	TVVPQNASARPACSL EECCCCCCCCCCCCC	32.15	25293948

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of WAC_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WAC_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WAC_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of WAC_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-533, AND MASSSPECTROMETRY.
PubMed: 17242355