dbPTM

VINC_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	VINC_MOUSE
UniProt AC	Q64727
Protein Name	Vinculin
Gene Name	Vcl
Organism	Mus musculus (Mouse).
Sequence Length	1066
Subcellular Localization	Cell membrane Peripheral membrane protein Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Cell membrane, sarcolemma Peripheral membrane protein Cytoplasmic side . Recruitment to
Protein Description	Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity)..
Protein Sequence	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
52	Phosphorylation	AAVQAAVSNLVRVGK HHHHHHHHHHHHCCC	21.51	-
59	Ubiquitination	SNLVRVGKETVQTTE HHHHHCCCCCCCCCH	47.56	22790023
71	Acetylation	TTEDQILKRDMPPAF CCHHHHHHCCCCHHH	48.24	22826441
80	Acetylation	DMPPAFIKVENACTK CCCHHHHHHHHHHHH	36.72	22826441
97	Phosphorylation	QAAQMLQSDPYSVPA HHHHHHHCCCCCCCH	36.27	27180971
100	Phosphorylation	QMLQSDPYSVPARDY HHHHCCCCCCCHHHE	27.56	27742792
101	Phosphorylation	MLQSDPYSVPARDYL HHHCCCCCCCHHHEE	27.32	27742792
170	Acetylation	NLGPGMTKMAKMIDE CCCCCHHHHHHHHHH	28.15	23806337
170	Malonylation	NLGPGMTKMAKMIDE CCCCCHHHHHHHHHH	28.15	26320211
170	Succinylation	NLGPGMTKMAKMIDE CCCCCHHHHHHHHHH	28.15	23806337
173	Acetylation	PGMTKMAKMIDERQQ CCHHHHHHHHHHHHH	32.65	22826441
183	Phosphorylation	DERQQELTHQEHRVM HHHHHHHCCCHHHHH	22.29	29899451
197	Phosphorylation	MLVNSMNTVKELLPV HHHHCHHHHHHHHHH	25.06	29899451
216	Ubiquitination	MKIFVTTKNSKNQGI HHHEEECCCCCCCCH	50.27	22790023
219	Ubiquitination	FVTTKNSKNQGIEEA EEECCCCCCCCHHHH	63.78	22790023
228	Ubiquitination	QGIEEALKNRNFTVE CCHHHHHHCCCCCHH	61.99	22790023
251	Phosphorylation	IIRVLQLTSWDEDAW HHHHHHCCCCCCCHH	18.14	25195567
260	Phosphorylation	WDEDAWASKDTEAMK CCCCHHHCCCHHHHH	21.99	22817900
261	Ubiquitination	DEDAWASKDTEAMKR CCCHHHCCCHHHHHH	62.01	22790023
272	Phosphorylation	AMKRALASIDSKLNQ HHHHHHHHHHHHHHH	27.98	26824392
275	Phosphorylation	RALASIDSKLNQAKG HHHHHHHHHHHHCCC	37.05	25521595
276	Acetylation	ALASIDSKLNQAKGW HHHHHHHHHHHCCCC	47.94	23806337
276	Succinylation	ALASIDSKLNQAKGW HHHHHHHHHHHCCCC	47.94	23806337
276	Ubiquitination	ALASIDSKLNQAKGW HHHHHHHHHHHCCCC	47.94	-
281	Ubiquitination	DSKLNQAKGWLRDPN HHHHHHCCCCCCCCC	40.64	22790023
290	Phosphorylation	WLRDPNASPGDAGEQ CCCCCCCCCCHHHHH	35.74	24925903
308	Ubiquitination	QILDEAGKVGELCAG HHHHHHCCHHHHHCC	54.86	22790023
316	Acetylation	VGELCAGKERREILG HHHHHCCHHHHHHHH	29.53	134199
316	Malonylation	VGELCAGKERREILG HHHHHCCHHHHHHHH	29.53	26320211
324	Phosphorylation	ERREILGTCKMLGQM HHHHHHHHHHHHHCC	12.90	17203969
326	Acetylation	REILGTCKMLGQMTD HHHHHHHHHHHCCHH	36.68	23236377
332	Phosphorylation	CKMLGQMTDQVADLR HHHHHCCHHHHHHHH	18.52	26643407
346	Phosphorylation	RARGQGASPVAMQKA HHCCCCCCHHHHHHH	27.08	25521595
352	Acetylation	ASPVAMQKAQQVSQG CCHHHHHHHHHHHHC	34.77	22826441
357	Phosphorylation	MQKAQQVSQGLDVLT HHHHHHHHHCHHHHH	17.75	27180971
364	Phosphorylation	SQGLDVLTAKVENAA HHCHHHHHHHHHHHH	25.04	26824392
366	Acetylation	GLDVLTAKVENAARK CHHHHHHHHHHHHHH	44.86	22826441
366	Ubiquitination	GLDVLTAKVENAARK CHHHHHHHHHHHHHH	44.86	22790023
378	Phosphorylation	ARKLEAMTNSKQSIA HHHHHHHHCCHHHHH	43.31	26824392
380	Phosphorylation	KLEAMTNSKQSIAKK HHHHHHCCHHHHHHH	23.94	26824392
381	Ubiquitination	LEAMTNSKQSIAKKI HHHHHCCHHHHHHHH	50.57	22790023
383	Phosphorylation	AMTNSKQSIAKKIDA HHHCCHHHHHHHHHH	28.40	26824392
387	Ubiquitination	SKQSIAKKIDAAQNW CHHHHHHHHHHHHHH	36.48	22790023
434	Phosphorylation	ERDDILRSLGEIAAL HHHHHHHHHHHHHHH	36.96	25521595
442	Phosphorylation	LGEIAALTSKLGDLR HHHHHHHHHHHHHHH	21.06	26824392
443	Phosphorylation	GEIAALTSKLGDLRR HHHHHHHHHHHHHHH	27.08	27180971
444	Acetylation	EIAALTSKLGDLRRQ HHHHHHHHHHHHHHC	51.78	22826441
444	Ubiquitination	EIAALTSKLGDLRRQ HHHHHHHHHHHHHHC	51.78	22790023
456	Phosphorylation	RRQGKGDSPEARALA HHCCCCCCHHHHHHH	33.05	26824392
464	Ubiquitination	PEARALAKQVATALQ HHHHHHHHHHHHHHH	46.27	22790023
475	Phosphorylation	TALQNLQTKTNRAVA HHHHHHHHHHHHHHH	42.90	25338131
476	Ubiquitination	ALQNLQTKTNRAVAN HHHHHHHHHHHHHHH	30.73	22790023
496	Acetylation	AAVHLEGKIEQAQRW HHHHHCHHHHHHHHH	33.42	23236377
537	Phosphorylation	ANVMMGPYRQDLLAK HCHHCCHHHHHHHHH	18.38	25195567
545	S-nitrosocysteine	RQDLLAKCDRVDQLT HHHHHHHCCHHHHHH	3.16	-
545	S-nitrosylation	RQDLLAKCDRVDQLT HHHHHHHCCHHHHHH	3.16	20925432
566	Phosphorylation	AARGEGESPQARALA HHCCCCCCHHHHHHH	32.92	26824392
574	Phosphorylation	PQARALASQLQDSLK HHHHHHHHHHHHHHH	32.07	24899341
579	Phosphorylation	LASQLQDSLKDLKAQ HHHHHHHHHHHHHHH	25.10	26824392
581	Acetylation	SQLQDSLKDLKAQMQ HHHHHHHHHHHHHHH	66.04	23236377
581	Ubiquitination	SQLQDSLKDLKAQMQ HHHHHHHHHHHHHHH	66.04	22790023
584	Ubiquitination	QDSLKDLKAQMQEAM HHHHHHHHHHHHHHH	46.86	22790023
596	Phosphorylation	EAMTQEVSDVFSDTT HHHHHHHHHHHCCCC	27.57	25619855
600	Phosphorylation	QEVSDVFSDTTTPIK HHHHHHHCCCCCCCH	33.62	27087446
602	Phosphorylation	VSDVFSDTTTPIKLL HHHHHCCCCCCCHHE	31.22	25619855
603	Phosphorylation	SDVFSDTTTPIKLLA HHHHCCCCCCCHHEH	35.25	25619855
604	Phosphorylation	DVFSDTTTPIKLLAV HHHCCCCCCCHHEHH	25.71	27087446
614	Phosphorylation	KLLAVAATAPPDAPN HHEHHHHCCCCCCCC	29.93	25338131
637	Phosphorylation	AANFENHSGRLGATA HHHCCCCCCCCCCHH	36.87	26824392
643	Phosphorylation	HSGRLGATAEKAAAV CCCCCCCHHHHHHHH	33.28	29514104
646	Acetylation	RLGATAEKAAAVGTA CCCCHHHHHHHHCCC	40.64	23806337
646	Succinylation	RLGATAEKAAAVGTA CCCCHHHHHHHHCCC	40.64	23806337
655	Ubiquitination	AAVGTANKSTVEGIQ HHHCCCCHHHHHHHH	45.07	22790023
656	Phosphorylation	AVGTANKSTVEGIQA HHCCCCHHHHHHHHH	37.18	26824392
664	Phosphorylation	TVEGIQASVKTAREL HHHHHHHHHHHHHHH	14.05	22871156
666	Ubiquitination	EGIQASVKTARELTP HHHHHHHHHHHHHCH	33.69	22790023
672	Phosphorylation	VKTARELTPQVISAA HHHHHHHCHHHHHHH	13.61	-
677	Phosphorylation	ELTPQVISAARILLR HHCHHHHHHHHHHHH	19.61	26824392
692	Phosphorylation	NPGNQAAYEHFETMK CCCCHHHHHHHHHHH	16.98	26824392
697	Phosphorylation	AAYEHFETMKNQWID HHHHHHHHHHHHHHH	32.28	29472430
699	Acetylation	YEHFETMKNQWIDNV HHHHHHHHHHHHHHH	54.10	23236377
699	Ubiquitination	YEHFETMKNQWIDNV HHHHHHHHHHHHHHH	54.10	22790023
710	Phosphorylation	IDNVEKMTGLVDEAI HHHHHHHHCCCCHHH	38.13	25777480
719	Phosphorylation	LVDEAIDTKSLLDAS CCCHHHCHHHHHHHC	19.20	26239621
720	Ubiquitination	VDEAIDTKSLLDASE CCHHHCHHHHHHHCH	35.04	22790023
721	Phosphorylation	DEAIDTKSLLDASEE CHHHCHHHHHHHCHH	35.94	25521595
726	Phosphorylation	TKSLLDASEEAIKKD HHHHHHHCHHHHHHH	35.32	26824392
731	Acetylation	DASEEAIKKDLDKCK HHCHHHHHHHHHHHC	47.48	23236377
731	Ubiquitination	DASEEAIKKDLDKCK HHCHHHHHHHHHHHC	47.48	22790023
754	Phosphorylation	QMLVAGATSIARRAN HHHHHCHHHHHHHHH	21.54	26824392
755	Phosphorylation	MLVAGATSIARRANR HHHHCHHHHHHHHHH	17.45	30352176
768	Malonylation	NRILLVAKREVENSE HHHEEEEEHHHCCCC	41.43	26320211
774	Phosphorylation	AKREVENSEDPKFRE EEHHHCCCCCHHHHH	29.85	17203969
778	Acetylation	VENSEDPKFREAVKA HCCCCCHHHHHHHHH	70.18	22826441
778	Ubiquitination	VENSEDPKFREAVKA HCCCCCHHHHHHHHH	70.18	22790023
784	Acetylation	PKFREAVKAASDELS HHHHHHHHHHHHHHH	45.33	7719587
784	Ubiquitination	PKFREAVKAASDELS HHHHHHHHHHHHHHH	45.33	22790023
787	Phosphorylation	REAVKAASDELSKTI HHHHHHHHHHHHHCC	36.16	26824392
792	Ubiquitination	AASDELSKTISPMVM HHHHHHHHCCCHHHH	63.60	22790023
793	Phosphorylation	ASDELSKTISPMVMD HHHHHHHCCCHHHHC	24.26	23984901
795	Phosphorylation	DELSKTISPMVMDAK HHHHHCCCHHHHCHH	16.51	26824392
802	Acetylation	SPMVMDAKAVAGNIS CHHHHCHHHHCCCCC	38.85	23236377
802	Ubiquitination	SPMVMDAKAVAGNIS CHHHHCHHHHCCCCC	38.85	22790023
809	Phosphorylation	KAVAGNISDPGLQKS HHHCCCCCCCHHHHH	41.58	25521595
815	Acetylation	ISDPGLQKSFLDSGY CCCCHHHHHHHHHHH	48.49	22826441
815	Ubiquitination	ISDPGLQKSFLDSGY CCCCHHHHHHHHHHH	48.49	22790023
816	Phosphorylation	SDPGLQKSFLDSGYR CCCHHHHHHHHHHHH	20.34	26824392
820	Phosphorylation	LQKSFLDSGYRILGA HHHHHHHHHHHHHHH	39.61	26239621
822	Phosphorylation	KSFLDSGYRILGAVA HHHHHHHHHHHHHHH	9.83	25521595
830	Ubiquitination	RILGAVAKVREAFQP HHHHHHHHHHHHHCC	34.91	22790023
913	Phosphorylation	HDEARKWSSKGNDII HHHHHHHHHCCHHHH	25.80	22817900
915	Acetylation	EARKWSSKGNDIIAA HHHHHHHCCHHHHHH	57.10	22826441
915	Malonylation	EARKWSSKGNDIIAA HHHHHHHCCHHHHHH	57.10	26320211
915	Ubiquitination	EARKWSSKGNDIIAA HHHHHHHCCHHHHHH	57.10	-
924	Acetylation	NDIIAAAKRMALLMA HHHHHHHHHHHHHHH	37.67	22826441
924	Ubiquitination	NDIIAAAKRMALLMA HHHHHHHHHHHHHHH	37.67	22790023
941	Phosphorylation	SRLVRGGSGTKRALI HHHHCCCCCHHHHHH	46.22	29514104
952	Acetylation	RALIQCAKDIAKASD HHHHHHHHHHHHCCH	58.24	23806337
952	Malonylation	RALIQCAKDIAKASD HHHHHHHHHHHHCCH	58.24	26320211
956	Malonylation	QCAKDIAKASDEVTR HHHHHHHHCCHHHHH	49.08	26320211
956	Ubiquitination	QCAKDIAKASDEVTR HHHHHHHHCCHHHHH	49.08	-
990	Phosphorylation	QVCERIPTISTQLKI HHHHHCCCHHHHHHH	25.78	27180971
993	Phosphorylation	ERIPTISTQLKILST HHCCCHHHHHHHHHH	33.79	26824392
996	Ubiquitination	PTISTQLKILSTVKA CCHHHHHHHHHHHHH	31.61	22790023
1002	Acetylation	LKILSTVKATMLGRT HHHHHHHHHHHCCCC	37.92	22826441
1002	Ubiquitination	LKILSTVKATMLGRT HHHHHHHHHHHCCCC	37.92	22790023
1033	Phosphorylation	NAQNLMQSVKETVRE HHHHHHHHHHHHHHH	22.61	-
1035	Ubiquitination	QNLMQSVKETVREAE HHHHHHHHHHHHHHH	53.57	22790023
1047	Acetylation	EAEAASIKIRTDAGF HHHHHCCEEEECCCC	24.52	22826441
1055	Phosphorylation	IRTDAGFTLRWVRKT EEECCCCEEEEHHCC	17.94	26824392
1065	Phosphorylation	WVRKTPWYQ------ EHHCCCCCC------	12.92	20006947

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1065	Y	Phosphorylation	Kinase	SRC-TYPE TYR-KINASES	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of VINC_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of VINC_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
RAVR1_MOUSE	Raver1	physical	11724819

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of VINC_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry."; Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.; Mol. Cell. Proteomics 8:904-912(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-290 AND SER-721, ANDMASS SPECTROMETRY.	19131326 [Abstract]
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-290 AND SER-721, ANDMASS SPECTROMETRY.	19367708 [Abstract]
"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry."; Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.; J. Proteome Res. 6:250-262(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-324 AND SER-774, ANDMASS SPECTROMETRY.	17203969 [Abstract]
"Specific phosphopeptide enrichment with immobilized titanium ionaffinity chromatography adsorbent for phosphoproteome analysis."; Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.; J. Proteome Res. 7:3957-3967(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-290, AND MASSSPECTROMETRY.	18630941 [Abstract]
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-822, AND MASSSPECTROMETRY.	18034455 [Abstract]