VCAM1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: VCAM1_MOUSE
• UniProt AC: P29533
• Protein Name: Vascular cell adhesion protein 1
• Gene Name: Vcam1
• Organism: Mus musculus (Mouse).
• Sequence Length: 739
• Subcellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor.
• Protein Description: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation..
• Protein Sequence: MPVKMVAVLGASTVLWILFAVSQAFKIEISPEYKTIAQIGDSMALTCSTTGCESPLFSWRTQIDSPLNAKVRTEGSKSVLTMEPVSFENEHSYLCTATCGSGKLERSIHVDIYSFPKDPEIQFSGPLEVGKPVTVKCLAPDIYPVYRLEIDLFKGDQLMNRQEFSSEEMTKSLETKSLEVTFTPVIEDIGKALVCRAKLHIDQIDSTLKERETVKELQVYISPRNTTISVHPSTRLQEGGAVTMTCSSEGLPAPEIFWGRKLDNEVLQLLSGNATLTLIAMRMEDSGVYVCEGVNLIGRDKAEVELVVQEKPFIVDISPGSQVAAQVGDSVVLTCAAIGCDSPSFSWRTQTDSPLNGVVRNEGAKSTLVLSSVGFEDEHSYLCAVTCLQRTLEKRTQVEVYSFPEDPVIKMSGPLVHGRPVTVNCTVPNVYPFDHLEIELLKGETTLMKKYFLEEMGIKSLETKILETTFIPTIEDTGKSLVCLARLHSGEMESEPKQRQSVQPLYVNVAPKETTIWVSPSPILEEGSPVNLTCSSDGIPAPKILWSRQLNNGELQPLSENTTLTFMSTKRDDSGIYVCEGINEAGISRKSVELIIQVSPKDIQLTVFPSKSVKEGDTVIISCTCGNVPETWIILKKKAKTGDMVLKSVDGSYTIRQAQLQDAGIYECESKTEVGSQLRSLTLDVKGKEHNKDYFSPELLALYCASSLVIPAIGMIVYFARKANMKGSYSLVEAQKSKV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
165	Phosphorylation	LMNRQEFSSEEMTKS HCCHHCCCHHHHHHH	35.77	25521595
166	Phosphorylation	MNRQEFSSEEMTKSL CCHHCCCHHHHHHHH	42.78	25521595
170	Phosphorylation	EFSSEEMTKSLETKS CCCHHHHHHHHHCCC	22.29	27742792
225	N-linked_Glycosylation	QVYISPRNTTISVHP EEEECCCCCEEEECC	44.98	19656770
264	N-linked_Glycosylation	FWGRKLDNEVLQLLS EECCCCCHHHHHHHC	52.27	19656770
273	N-linked_Glycosylation	VLQLLSGNATLTLIA HHHHHCCCCEEEEEE	26.78	19656770
319 (in isoform 2)	GPI-anchor	-	46.95	7687058
346	Phosphorylation	GCDSPSFSWRTQTDS CCCCCCCCCCCCCCC	21.71	28059163
424	N-linked_Glycosylation	HGRPVTVNCTVPNVY CCCCEEEECCCCCEE	13.33	-
494	Phosphorylation	LHSGEMESEPKQRQS HHCCCCCCCCCCCCC	58.87	-
531	N-linked_Glycosylation	LEEGSPVNLTCSSDG CCCCCCCEEEECCCC	32.77	-
552	N-linked_Glycosylation	LWSRQLNNGELQPLS EEEEECCCCCCCCCC	54.64	19656770
561	N-linked_Glycosylation	ELQPLSENTTLTFMS CCCCCCCCCEEEEEC	34.68	19656770
680	Phosphorylation	EVGSQLRSLTLDVKG CCCHHHEEEEEECCC	34.01	28833060
729	Phosphorylation	KANMKGSYSLVEAQK HCCCCCCCCHHHHHH	18.07	29514104
730	Phosphorylation	ANMKGSYSLVEAQKS CCCCCCCCHHHHHHC	27.86	20450229
736	Ubiquitination	YSLVEAQKSKV---- CCHHHHHHCCC----	60.33	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of VCAM1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of VCAM1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of VCAM1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of VCAM1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

N-linked Glycosylation

"The mouse C2C12 myoblast cell surface N-linked glycoproteome:identification, glycosite occupancy, and membrane orientation.";
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I.,Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E.,Wollscheid B.;
Mol. Cell. Proteomics 8:2555-2569(2009).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-225; ASN-264; ASN-273;ASN-552 AND ASN-561, AND MASS SPECTROMETRY.
PubMed: 19656770