UCHL3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UCHL3_MOUSE
• UniProt AC: Q9JKB1
• Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Gene Name: Uchl3
• Organism: Mus musculus (Mouse).
• Sequence Length: 230
• Subcellular Localization: Cytoplasm .
• Protein Description: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome..
• Protein Sequence: MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMEPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERAKFLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGKTSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
61	Ubiquitination	LLFPITEKYEVFRTE HHEECCCCEEECCCH	37.75	-
74	Ubiquitination	TEEEEKIKSQGQDVT CHHHHHHHHCCCCHH	48.04	22790023
95	Glutathionylation	KQTISNACGTIGLIH HHHHHHHHHHHHHHH	6.05	24333276
95	S-palmitoylation	KQTISNACGTIGLIH HHHHHHHHHHHHHHH	6.05	28680068
115	Phosphorylation	KDKMHFESGSTLKKF CCCCCCCCCHHHHHH	36.78	28464351
121	Ubiquitination	ESGSTLKKFLEESVS CCCHHHHHHHHHHHC	59.29	22790023
126	Phosphorylation	LKKFLEESVSMSPEE HHHHHHHHHCCCHHH	15.32	26239621
128	Phosphorylation	KFLEESVSMSPEERA HHHHHHHCCCHHHHH	23.87	26239621
130	Phosphorylation	LEESVSMSPEERAKF HHHHHCCCHHHHHHH	22.96	27742792
136	Ubiquitination	MSPEERAKFLENYDA CCHHHHHHHHHHCCE	57.62	22790023
161	Phosphorylation	EGQTEAPSIDEKVDL CCCCCCCCCCCCCCE	50.12	26824392
165	Ubiquitination	EAPSIDEKVDLHFIA CCCCCCCCCCEEEEE	36.98	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of UCHL3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of UCHL3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UCHL3_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of UCHL3_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry.";
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.;
J. Proteome Res. 6:250-262(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, AND MASSSPECTROMETRY.
PubMed: 17203969