UBR4_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UBR4_RAT
• UniProt AC: Q2TL32
• Protein Name: E3 ubiquitin-protein ligase UBR4
• Gene Name: Ubr4
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 5194
• Subcellular Localization: Membrane ; Multi-pass membrane protein . Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility..
• Protein Description: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. Mediates ubiquitination of ACLY, leading to its subsequent degradation (By similarity)..
• Protein Sequence: MATSGGEEAAAAAPAPGAPATGQDTTPGWEVAVRPLLSASYSAFEMKELPQLVASVIESESEILHHEKQYEPFYSSFVALSTHYITTVCSLIPRNQLQSVAAACKVLIEFSLLRLENPDEACAVSQKHLILLIKGLCTGCSRLDRTEIITFTAMMKSAKLPQTVKTLSDVEDQKELASPVSPELRQKEVQMNFLNQLTSVFNPRTVPSPPISPQALVEGENDEQSSTEQASAVKTKNVFIAQNVASLQELGGSEKLLRVCLNLPYFLRYINRFQDAVVANSFFIMPATVADATAVRNGFHSLVIDVTMALDTLSLPVLEPLNPSRLQDVTVLSLSCLYAGVSVATCMAILHVGSAQQVRTGSTSSKEEDYESDAATIVQKCLEIYDMIGQAISSSRRAGGEHFQNFQLLGAWCLLNSLFLILHLSPTALADKGKEKDPLAALRVRDILSRTKEGVGSPKLGPGKGHQGFGVLSVILANHAIKLLTSLFQDLQVEALHKGWETDGPPAVLSIMAQSTSTQRVQRLIDSVPLTNLLLTLLSTSYRKACVLQRQRKGSMSSDASASTDSNTYYEDDFSSTEEDSSQDDDSEPILGQWFEETISPSKEKAAPPPPPPPPPLESSPRVKSPSKQASGEKGNILASRKDPELFLGLASNILNFITSSMLNSRNNFIRNYLSVSLSEQHMATLASIIKEVDKDGLKGSSDEDFAAALYHFNHSLVTSDLQSPNLQNTLLQQLGVAPLSSGPWPLYIHPQGLSVLSRLLLIWQHKAGAQGDPDVPECLKVWDRFLTTMKQSALQGVVPSETEDLNIEHLQLLLLIFHSFSEKGRRAILTTLVQSIQELSVNMEVQMRSAPLILARLLLIFDYLLHQYSKAPVYLFEQVQHNLLSPPFGWASGSQDSSSSSRRANIPLYHGFKEVEENWSKHFSSDAAPQPRFYCVLSTEASEEDLNRLDSEACEVLFSKLVKYDELYSSLTALLAAGSQLDTVKRKENKNMTALEACALQYYFLILWRILGILPPSKTYMNQLAMNSPEMSECDILHTLRWSSRLRISSYVSWIKDHLIRQGMRPEHAGSLVELAASKCSSVKYDVEIVEEYFARQISSFCSIDCTTVLQLHEIPSLQSIYTLDAAVSKVQVSLDEHFSKMAAETDPHKSSEITKNLLPATLQLIDTYASFTRAYLLQNLNEEGSTEKPSQEKLHGFAAVLAIGSSRCKANTLGPTLVQNLPSSVQSVCESWNNINTNEFPNIGSWRNAFANDTIPSESYISAVQAAHLGTLCGQSLPLAASLKHALLSLVRLTGDLIVWSDEMNPAQVIRALLPLLLESSTESAAEISSNSLERILGPAESDEFLARVYEKLITGCYNILANHADPSSGLDESVLEECLQYLEKQLESSQARKAMEEFFSDGGELVQIMMATANEDLSAKFCNRVLKFFTKLFQLTEKSPNPSLLHLCGSLAQLACVEPVRLQAWLTRMTTSPPKDSDQLEVIQENRQLLQLLTTYIVRENSQVGEGVCAVLLGTLTPMATDMLANGDGTGFPELMVVMATLASAGQGAGHLQLHNAAVDWLGRCKKYLSQKNVVEKLNANVMHGKHVMVLECTCHIMSYLADVTNALSQSNGQGPSHLSVDGEERAIEVDSDWVEELAVEEEDSQAEDSDEDSLCNKLCTFTITQKEFMNQHWYHCHTCKMVDGVGVCTVCAKVCHKDHEISYAKYGSFFCDCGAKEDGSCLALVKRTPSSGMSSTMKESAFQSEPRVSESLVRHASTSPADKAKVTISDGKVADEEKPKKSSLCRTVEGCREELQNQANFSFAPLVLDMLNFLMDAIQTNFQQASAVGSSSRAQQALSELHTVDKVVEMTDQLMVPTLGSQEGAFENVRMNYSGDQGQTIRQLISAHVLRRVAMCVLSSPHGRRQHLAVSHEKGKITVLQLSALLKQADSSKRKLTLTRLASAPVPFTVLSLTGNPCKEDYLAVCGLKDCHVLTFSSSGSVSDHLVLHPQLATGNFIIKAVWLPGSQTELAIVTADFVKIYDLSVDALSPTFYFLLPSSKIRDVTFLFNEEGKNIIVIMSSAGYMYTQLMEEASSAQQGPFYVTNVLEINHEDLKDSNSQVAGGGVSVYYSHVLQMLFFSYSQGKSFAATVSRSTLEVLQLFPINIKSSNGGSKTSPALCQWSEVMNHPGLVCCVQQTTGVPLVVMVKPDTFLIQEIKTLPAKAKIQDMVAIRHTACNEQQRTTMILLCEDGSLRIYMANVENTSYWLQPSLQPSSVISIMKPVRKRKTATITARTSSQVTFPIDFFEHNQQLTDVEFGGNDLLQVYNAQQIKHRLNSTGMYVANTKPGGFTMEISNNSSTMVMTGMRIQIGTQAIERAPSYIEIFGRTMQLNLSRSRWFDFPFTREEALQADRKLNLFIGASVDPAGVTMIDAVKIYGKTKEQFGWPDEPPEDFPSASVSSVCPPNLNQSNSTGDSDSAAPATTSGTVLERLVVSSLEALESCFAVGPIIEKERNKHAAQELATLLLSLPAPASVQQQSKSLLASLHSSRSAYHSHKDQALLSKAVQCLNTSSKEGKDLDPEVFQRLVITARSIAVTRPNNLVHFTESKLPQMETEGAEEGKEPQKQVEGDGCSFITQLVNHFWKLHASKPKNAFLAPACLPGLTHIEATVNALVDIIHGYCTCELDCINTASKIYMQMLLCPDPAVSFSCKQALIRVLRPRNKRRHVTLPSSPRSNTPMGDKDDDDDDDADEKMQSSGIPDGGHIRQESQEQSEVDHGDFEMVSESMVLETAENVNNGNPSPLEALLAGAEGFPPMLDIPPDADDETMVELAIALSLQQDQQGSSSSALGLQSLGLSGQAPSSSSLDAGTLSDTTASGVCACNSSVTLSAPASDDEGSTAATDGSTLRTSPADHGGSVGSESGGSAVDSVAGEHSVSGRSSAYGDATAEGHPAGPGSVSSSTGAISTTTGHQEGDGSEGEGEGEAEGDVHTSNRLHMVRLMLLERLLQTLPQLRNVGGVRAIPYMQVILMLTTDLDGEDEKDKGALDNLLAQLIAELGMDKKDVSKKNERSALNEVHLVVMRLLSVFMSRTKSGSKSSICESSSLISSATAAALLSSGAVDYCLHVLKSLLEYWKGQQSEEEPVTTSQLLKPHTTSSPPDMSPFFLRQYVKGHAADVFEAYTQLLTEMVLRLPYQIKKIADTSSRIPPPVFDHSWFYFLSEYLMIQQTPFVRRQVRKLLLFICGSKEKYRQLRDLHTLDSHVRGIKKLLEEQGIFLRASVVTASSGSALQYDTLISLMEHLKACAEIAAQRTINWQKFCIKDDSVLYFLLQVSFLVDEGVSPVLLQLLSCALCGSKVLAALAASTGSSSVASSAPPAASSGQTTTQSKSSTKKSKKEKKEKEKEGESSGSQEDQLCTALVNQLNRFADKETLIQFLRCFLLESNSSSVRWQAHCLTLHIYRNSNKAQQELLLDLMWSIWPELPAYGRKAAQFVDLLGYFSLKTAQTEKKLKEYSQKAVEILRTQNHILTNHPNSNIYNTLSGLVEFDGYYLESDPCLVCNNPEVPFCYIKLSSIKVDTRYTTTQQVVKLIGSHTISKVTVKIGDLKRTKMVRTINLYYNNRTVQAIVELKNKPARWHKAKKVQLTPGQTEVKIDLPLPIVASNLMIEFADFYENYQASTETLQCPRCSASVPANPGVCGNCGENVYQCHKCRSINYDEKDPFLCNACGFCKYARFDFMLYAKPCCAVDPIENEEDRKKAVSNINTLLDKADRVYHQLMGHRPQLENLLCKVNEAAPEKPQEDSGTAGGISSTSASVNRYILQLAQEYCGDCKNSFDELSKIIQKVFASRKELLEYDLQQREAATKSSRTSVQPTFTASQYRALSVLGCGHTSSTKCYGCASAVTEHCITLLRALATNPALRHILVSQGLIRELFDYNLRRGAAAIREEVRQLMCLLTRDNPEATQQMNDLIIGKVSTALKGHWANPDLASSLQYEMLLLTDSISKEDSCWELRLRCALSLFLMAVNIKTPVVVENITLMCLRILQKLIKPPAPTSKKNKDVPVEALTTVKPYCNEIHAQAQLWLKRDPKASYEAWKKCLPIRGVDGNGKSPSKSELHRLYLTEKYVWRWKQFLSRRGKRTTPLDLKLGHNNWLRQVLFTPATQAARQAACTIVEALASVPSRKQQVLDLLTSYLDELSVAGECAAEYLALYQKLIASCHWKVYLAARGVLPYVGNLITKEIARLLALEEATLSTDLQQGYALKSLTGLLSSFVEVESIKRHFKSRLVGTVLNGYLCLRKLVLQRTKLIDETQDMLLEMLEDMTTGTESETKAFMAVCIETAKRYNLDDYRTPVFIFERLCSIIYPEENEVTEFFVTLEKDPQQEDFLQGRMPGNPYSSNEPGIGPLMRDIKNKICQDCDLVALLEDDSGMELLVNNKIISLDLPVAEVYKKVWCTTNEGEPMRIVYRMRGLLGDATEEFIESLDSTTDEEEDEEEVYRMAGVMAQCGGLQCMLNRLAGVKDFKQGRHLLTVLLKLFSYCVKVKVNRQQLVKLEMNTLNVMLGTLNLALVAEQESKDSGGAAVAEQVLSIMEIILDESNAEPLSEDKGNLLLTGDKDQLVMLLDQINSTFVRSNPSVLQGLLRIIPYLSFGEVEKMQILVERFKPYCSFDKYDEDHSGDDKVFLDCFCKIAAGIKNNSNGHQLKDLILQKGITQSALDYMKKHIPSAKNLDADIWKKFLSRPALPFILRLLRGLAMQHPATQVLIGTDSITSLHKLEQVSSDEGIGTLAENLLEALREHPDVNKKIDAARRETRAEKKRMAMAMRQKALGTLGMTTNEKGQVVTKTALLKQMEELIEEPGLTCCICREGYKFQPTKVLGIYTFTKRVALEEMENKPRKQQGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAFATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLIPYIIHTVLYVLNTTRATSREEKNLQGFLEQPREKWTESAFDVDGPHYFTILALHVLPPEQWKATRVEILRRLLVASHARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNMFKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDAAGLLSEITDPESFLKDLLNSVP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
166	Phosphorylation	KLPQTVKTLSDVEDQ CCCCHHCCCCHHHHH	27.45	28432305
168	Phosphorylation	PQTVKTLSDVEDQKE CCHHCCCCHHHHHHH	44.86	28432305
178	Phosphorylation	EDQKELASPVSPELR HHHHHHCCCCCHHHH	38.16	29779826
181	Phosphorylation	KELASPVSPELRQKE HHHCCCCCHHHHHHH	18.95	27097102
208	Phosphorylation	FNPRTVPSPPISPQA CCCCCCCCCCCCHHH	38.68	28689409
212	Phosphorylation	TVPSPPISPQALVEG CCCCCCCCHHHHCCC	19.41	28689409
253	Phosphorylation	SLQELGGSEKLLRVC HHHHCCCCHHHHHHH	29.74	27097102
360	Phosphorylation	GSAQQVRTGSTSSKE CCCCCCCCCCCCCCC	36.31	21630457
362	Phosphorylation	AQQVRTGSTSSKEED CCCCCCCCCCCCCCH	25.22	30240740
363	Phosphorylation	QQVRTGSTSSKEEDY CCCCCCCCCCCCCHH	38.06	22668510
364	Phosphorylation	QVRTGSTSSKEEDYE CCCCCCCCCCCCHHH	40.90	21630457
365	Phosphorylation	VRTGSTSSKEEDYES CCCCCCCCCCCHHHH	44.14	21630457
370	Phosphorylation	TSSKEEDYESDAATI CCCCCCHHHHHHHHH	22.17	22668510
372	Phosphorylation	SKEEDYESDAATIVQ CCCCHHHHHHHHHHH	26.73	25575281
457	Phosphorylation	RTKEGVGSPKLGPGK HCCCCCCCCCCCCCC	19.22	29779826
619	Phosphorylation	PPPPPLESSPRVKSP CCCCCCCCCCCCCCC	54.01	29779826
620	Phosphorylation	PPPPLESSPRVKSPS CCCCCCCCCCCCCCC	13.83	27097102
631	Phosphorylation	KSPSKQASGEKGNIL CCCCCCCCCCCCCCC	45.02	26437020
1085	Acetylation	ASKCSSVKYDVEIVE HHHCCCCCCHHHHHH	37.37	-
1151	Ubiquitination	AAETDPHKSSEITKN HHCCCCCCCCHHHHC	62.15	-
1403	Phosphorylation	KAMEEFFSDGGELVQ HHHHHHHCCHHHHHH	40.44	16641100
1635	Phosphorylation	ERAIEVDSDWVEELA CCEEEECCHHHHHHH	38.25	28689409
1648	Phosphorylation	LAVEEEDSQAEDSDE HHCCCCCCCCCCCCH	34.27	28689409
1653	Phosphorylation	EDSQAEDSDEDSLCN CCCCCCCCCHHHHHH	34.11	28689409
1657	Phosphorylation	AEDSDEDSLCNKLCT CCCCCHHHHHHHHHE	32.54	28689409
1748	Phosphorylation	MKESAFQSEPRVSES HHHHHCCCCCCCCHH	43.01	-
1755	Phosphorylation	SEPRVSESLVRHAST CCCCCCHHHHHHCCC	25.34	22673903
1761	Phosphorylation	ESLVRHASTSPADKA HHHHHHCCCCHHHHC	24.24	25403869
1773	Phosphorylation	DKAKVTISDGKVADE HHCCEEECCCCCCCC	30.83	22673903
1878	Phosphorylation	ENVRMNYSGDQGQTI CCEECCCCCCCHHHH	30.78	28432305
1904	Phosphorylation	VAMCVLSSPHGRRQH HHHHHHCCCCCCCCE	19.43	-
1931	Acetylation	LQLSALLKQADSSKR HHHHHHHHHCCCCCC	44.27	22902405
2715	Phosphorylation	RNKRRHVTLPSSPRS CCCCCCCCCCCCCCC	27.16	27097102
2718	Phosphorylation	RRHVTLPSSPRSNTP CCCCCCCCCCCCCCC	56.96	23712012
2719	Phosphorylation	RHVTLPSSPRSNTPM CCCCCCCCCCCCCCC	23.53	23712012
2722	Phosphorylation	TLPSSPRSNTPMGDK CCCCCCCCCCCCCCC	48.73	21738781
2724	Phosphorylation	PSSPRSNTPMGDKDD CCCCCCCCCCCCCCC	18.85	29779826
2743	Phosphorylation	DADEKMQSSGIPDGG CHHHHHHHCCCCCCC	27.34	30181290
2744	Phosphorylation	ADEKMQSSGIPDGGH HHHHHHHCCCCCCCC	24.69	30181290
2896	Phosphorylation	TDGSTLRTSPADHGG CCCCCCCCCCCCCCC	42.02	21738781
2897	Phosphorylation	DGSTLRTSPADHGGS CCCCCCCCCCCCCCC	16.20	27097102
2904	Phosphorylation	SPADHGGSVGSESGG CCCCCCCCCCCCCCC	28.09	27097102
2907	Phosphorylation	DHGGSVGSESGGSAV CCCCCCCCCCCCCCC	26.83	27097102
2909	Phosphorylation	GGSVGSESGGSAVDS CCCCCCCCCCCCCHH	50.59	27097102
2912	Phosphorylation	VGSESGGSAVDSVAG CCCCCCCCCCHHCCC	28.75	27097102
2916	Phosphorylation	SGGSAVDSVAGEHSV CCCCCCHHCCCCCCC	13.78	27097102
2922	Phosphorylation	DSVAGEHSVSGRSSA HHCCCCCCCCCCCCC	17.42	27097102
2924	Phosphorylation	VAGEHSVSGRSSAYG CCCCCCCCCCCCCCC	31.35	22673903
2934	Phosphorylation	SSAYGDATAEGHPAG CCCCCCCCCCCCCCC	29.95	21630457
2944	Phosphorylation	GHPAGPGSVSSSTGA CCCCCCCCCCCCCCC	23.20	28689409
2946	Phosphorylation	PAGPGSVSSSTGAIS CCCCCCCCCCCCCEE	21.92	28689409
2953	Phosphorylation	SSSTGAISTTTGHQE CCCCCCEEEECCCCC	21.11	28689409
2954	Phosphorylation	SSTGAISTTTGHQEG CCCCCEEEECCCCCC	23.43	28689409
2955	Phosphorylation	STGAISTTTGHQEGD CCCCEEEECCCCCCC	24.80	22668510
2956	Phosphorylation	TGAISTTTGHQEGDG CCCEEEECCCCCCCC	32.52	28689409
2964	Phosphorylation	GHQEGDGSEGEGEGE CCCCCCCCCCCCCCC	47.36	22668510
3232	Phosphorylation	LLLFICGSKEKYRQL HHHHHHCCHHHHHHH	32.98	24972320
3827	Acetylation	NSFDELSKIIQKVFA CCHHHHHHHHHHHHH	57.35	22902405
3871	Phosphorylation	ASQYRALSVLGCGHT HHHHHHHHEECCCCC	17.82	23984901
3878	Phosphorylation	SVLGCGHTSSTKCYG HEECCCCCCCCCCCC	15.06	23984901
4383	Phosphorylation	GRMPGNPYSSNEPGI CCCCCCCCCCCCCCC	28.82	-
4684	Phosphorylation	AAGIKNNSNGHQLKD HHCCCCCCCCHHHHH	54.29	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of UBR4_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of UBR4_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UBR4_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of UBR4_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.