dbPTM

UBP16_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	UBP16_MOUSE
UniProt AC	Q99LG0
Protein Name	Ubiquitin carboxyl-terminal hydrolase 16 {ECO:0000255\|HAMAP-Rule:MF_03062}
Gene Name	Usp16
Organism	Mus musculus (Mouse).
Sequence Length	825
Subcellular Localization	Nucleus .
Protein Description	Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. [PubMed: 24034696 Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.]
Protein Sequence	MGKKRTKGRSAPDTVASESAEPVCRHLRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKPEEEAEDPSVWLCLKCGHQGCGRDSQEQHALKHYTTPRSEPHYLVLSLDNWSVWCYKCDEEVKYCSSNRLGQVVDYVRKQAGVRTSKPAEKNNGHIELENKKLEKESKNEQEREKSENLAKETIPMDSASQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEVNLEPPGPLTLAMSQFLSEMQENKKRVVTPKELFSQVCKKATRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEEVKNKVKDYEKKKAIPSFVDRIFGGELTSTIMCDECRTVSLVHESFLDLSLPVLDDQSGKKSINDKNVKMTMEEEDKDSEEEKDDSYMKSRSDLPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQERFLHFNELCATDYTEDNEREADTALAGEVEVDTDSTHGSQEEATQIELSVNQKDLDGQESMIERTPDVQESPEDLGVKSANTESDLGIVTPAPECPRDFNGAFLEERTSGELDIINGLKNLNLNAAVDPDEINIEIPNDSHSAPKVYEVMNEDPETAFCTLANREAFSTDECSIQHCLYQFTRNEKLQDANKLLCEVCSRRQCNGPKANIKGDRRHVYTNAKKQMLVSLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEESTRVLYSLYGVVEHSGTMRSGHYTAYAKERTASCHLSNLVLHGDIPQDCEMESTKGQWFHISDTHVQAVPITKVLNSQAYLLFYERIL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
17	Phosphorylation	SAPDTVASESAEPVC CCCCCHHHCCHHHHH	27.96	23567750
37	Ubiquitination	GLEQGNLKKALVNVE HHHHCCHHHHHHCCE	39.75	27667366
188	Phosphorylation	KETIPMDSASQITVK HHCCCCCCCHHEEEE	24.46	-
278	Ubiquitination	KKRVVTPKELFSQVC CCCCCCHHHHHHHHH	58.65	22790023
278	Ubiquitination	KKRVVTPKELFSQVC CCCCCCHHHHHHHHH	58.65	-
292	Ubiquitination	CKKATRFKGYQQQDS HHHHHCCCCCCCCCH	54.11	22790023
292	Ubiquitination	CKKATRFKGYQQQDS HHHHHCCCCCCCCCH	54.11	-
294	Phosphorylation	KATRFKGYQQQDSQE HHHCCCCCCCCCHHH	12.07	23140645
299	Phosphorylation	KGYQQQDSQELLRYL CCCCCCCHHHHHHHH	22.63	23140645
406	Phosphorylation	NDKNVKMTMEEEDKD CCCCCEEECCHHHCC	18.73	25619855
414	Phosphorylation	MEEEDKDSEEEKDDS CCHHHCCCHHHHCCH	53.28	25521595
422	Phosphorylation	EEEKDDSYMKSRSDL HHHHCCHHHHHHHHC	18.63	25619855
520	Phosphorylation	KDLDGQESMIERTPD HHCCCCHHHHHCCCC	19.71	25521595
525	Phosphorylation	QESMIERTPDVQESP CHHHHHCCCCCCCCH	15.85	30635358
531	Phosphorylation	RTPDVQESPEDLGVK CCCCCCCCHHHHCCC	19.25	26824392
569	Phosphorylation	AFLEERTSGELDIIN CCCCCCCCCCCCCCC	36.17	-
683	Acetylation	HVYTNAKKQMLVSLA HCCCCHHHHHHHHHC	38.23	2416033
743	Phosphorylation	EESTRVLYSLYGVVE HHHHHHHHHHHHHHH	8.18	-
744	Phosphorylation	ESTRVLYSLYGVVEH HHHHHHHHHHHHHHC	15.79	23140645

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of UBP16_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of UBP16_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of UBP16_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
H2A2C_HUMAN	HIST2H2AC	physical	24034696

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of UBP16_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-414, AND MASSSPECTROMETRY.	19367708 [Abstract]