UBP15_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UBP15_MOUSE
• UniProt AC: Q8R5H1
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 15
• Gene Name: Usp15
• Organism: Mus musculus (Mouse).
• Sequence Length: 981
• Subcellular Localization: Cytoplasm . Nucleus . Mitochondrion .
• Protein Description: Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal. Able to mediate deubiquitination of monoubiquitinated substrates as well as 'Lys-48'-linked polyubiquitin chains, protecting them against proteasomal degradation. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B. Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy. Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP. Involved in endosome organization by mediating deubiquitination of SQSTM1: ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery..
• Protein Sequence: MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFNIPDEKEARLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPKSPGASNFSTLPKISPSSLSNNYNNINNRNVKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERSLEVYLVRMDPLAKPMQYKVIVPKIGNILDLCTALSALSGVPADKMIVTDIYNHRFHRIFAVDENLSSIMERDDIYVFEININRAEDTEHVVIPVCLREKFRHSSYTHHTGSSLFGQPFLMAIPRNNTEDKLYNLLLLRMCRYVKMSTETEETDGHLRCCEDQNINGNGPNGLHEEGSPSEMETDEPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEETCKGQLTGHKKRLFTFQFNNLGNNDINYIKDDTSHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDFEKHESVEYKPPKRPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPISDLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDLENENCMHTN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAEGGAADL ------CCCCCCCCC	24.92	-
154	Ubiquitination	VVTRRFSKADTIDTI CEEECCCCCCCHHHH	47.79	22790023
193	Ubiquitination	NTFEPLNKPDSTIQD CCCCCCCCCCCCCCC	58.86	22790023
219	Phosphorylation	EQKNEDGTWPRGPST EEECCCCCCCCCCCC	44.62	-
225	Phosphorylation	GTWPRGPSTPKSPGA CCCCCCCCCCCCCCC	61.23	22942356
225 (in isoform 5)	Phosphorylation	-	61.23	23375375
226 (in isoform 5)	Phosphorylation	-	37.56	25263469
226 (in isoform 2)	Phosphorylation	-	37.56	23375375
226	Phosphorylation	TWPRGPSTPKSPGAS CCCCCCCCCCCCCCC	37.56	26824392
227 (in isoform 2)	Phosphorylation	-	39.19	25263469
228	Ubiquitination	PRGPSTPKSPGASNF CCCCCCCCCCCCCCC	70.17	22790023
229	Phosphorylation	RGPSTPKSPGASNFS CCCCCCCCCCCCCCC	29.75	26824392
231 (in isoform 2)	Ubiquitination	-	49.05	-
233	Phosphorylation	TPKSPGASNFSTLPK CCCCCCCCCCCCCCC	44.30	25521595
234 (in isoform 5)	Phosphorylation	-	34.06	29514104
235 (in isoform 2)	Phosphorylation	-	5.67	29514104
236	Phosphorylation	SPGASNFSTLPKISP CCCCCCCCCCCCCCH	33.03	25619855
237	Phosphorylation	PGASNFSTLPKISPS CCCCCCCCCCCCCHH	42.83	25619855
242	Phosphorylation	FSTLPKISPSSLSNN CCCCCCCCHHHCCCC	25.51	26824392
244	Phosphorylation	TLPKISPSSLSNNYN CCCCCCHHHCCCCCC	36.80	25168779
245	Phosphorylation	LPKISPSSLSNNYNN CCCCCHHHCCCCCCC	38.92	25619855
247	Phosphorylation	KISPSSLSNNYNNIN CCCHHHCCCCCCCCC	25.61	25619855
250	Phosphorylation	PSSLSNNYNNINNRN HHHCCCCCCCCCCCC	17.52	25619855
259	Ubiquitination	NINNRNVKNSNYCLP CCCCCCCCCCCCCCC	59.20	22790023
263	Phosphorylation	RNVKNSNYCLPSYTA CCCCCCCCCCCCCCE	8.66	29514104
274	Phosphorylation	SYTAYKNYDYSEPGR CCCEECCCCCCCCCC	16.47	25367039
276	Phosphorylation	TAYKNYDYSEPGRNN CEECCCCCCCCCCCC	12.46	25367039
363	Ubiquitination	YVTPRAFKTQVGRFA EECCHHHHHCCCCCC	36.74	22790023
541	Phosphorylation	FAVDENLSSIMERDD EEECCCHHHHHHHCC	29.42	23984901
542	Phosphorylation	AVDENLSSIMERDDI EECCCHHHHHHHCCE	29.17	23984901
562	Phosphorylation	NINRAEDTEHVVIPV EEECCCCCCCEEEEE	21.35	-
602	Phosphorylation	MAIPRNNTEDKLYNL EEECCCCCHHHHHHH	49.46	22817900
605	Ubiquitination	PRNNTEDKLYNLLLL CCCCCHHHHHHHHHH	46.79	22790023
607	Phosphorylation	NNTEDKLYNLLLLRM CCCHHHHHHHHHHHH	14.98	25168779
621	Phosphorylation	MCRYVKMSTETEETD HHHHHCCCCCCCCCC	19.85	22817900
622	Phosphorylation	CRYVKMSTETEETDG HHHHCCCCCCCCCCC	43.35	20415495
624	Phosphorylation	YVKMSTETEETDGHL HHCCCCCCCCCCCCE	38.93	20415495
627	Phosphorylation	MSTETEETDGHLRCC CCCCCCCCCCCEEEC	40.84	20415495
696	Phosphorylation	DSENGLCTEETCKGQ CCCCCCCCCHHHCCC	41.73	-
699	Phosphorylation	NGLCTEETCKGQLTG CCCCCCHHHCCCCCC	17.18	-
705	Phosphorylation	ETCKGQLTGHKKRLF HHHCCCCCCCCCEEE	29.38	-
758	Ubiquitination	LDWDPDLKKRYFDEN CCCCHHHHHHHCCCC	42.45	22790023
847	Ubiquitination	YSRYMRDKLDTLVDF HHHHHHHHHCCCCCC	37.59	-
881	Phosphorylation	RYNLIAVSNHYGGMG EEEEEEEECCCCCCC	14.63	-
952	Phosphorylation	DRETKGASAATGIPL CCCCCCCCCCCCCCC	27.40	25159016
955	Phosphorylation	TKGASAATGIPLESD CCCCCCCCCCCCCCC	35.05	25521595
961	Phosphorylation	ATGIPLESDEDSNDN CCCCCCCCCCCCCCC	54.72	25521595
965	Phosphorylation	PLESDEDSNDNDNDL CCCCCCCCCCCCCCC	43.99	25521595
980	Phosphorylation	ENENCMHTN------ CCCCCCCCC------	0.00	25159016

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
678	S	Phosphorylation	Kinase	ATM	Q62388	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of UBP15_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UBP15_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SART3_HUMAN	SART3	physical	24984263

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.