UBA1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UBA1_RAT
• UniProt AC: Q5U300
• Protein Name: Ubiquitin-like modifier-activating enzyme 1 {ECO:0000250|UniProtKB:Q02053}
• Gene Name: Uba1 {ECO:0000312|EMBL:AAH85791.1, ECO:0000312|RGD:1359327}
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1058
• Subcellular Localization: Cytoplasm . Mitochondrion . Nucleus .
• Protein Description: Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites..
• Protein Sequence: MSSSPLSKKRRVSGPDPKPGSNCSSAQSVLSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNSPLEEQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMVQLNGCQPIEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHQFCAQHNRPPRPRNEEDATELVTLAQAVNARSPPAVQQDNVDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSSSPLSKK ------CCCCCCCCC	51.32	30181290
2	Acetylation	------MSSSPLSKK ------CCCCCCCCC	51.32	-
3	Phosphorylation	-----MSSSPLSKKR -----CCCCCCCCCC	48.08	22673903
4	Phosphorylation	----MSSSPLSKKRR ----CCCCCCCCCCC	38.87	22673903
7	Phosphorylation	-MSSSPLSKKRRVSG -CCCCCCCCCCCCCC	39.24	30181290
13	Phosphorylation	LSKKRRVSGPDPKPG CCCCCCCCCCCCCCC	42.68	29779826
21	Phosphorylation	GPDPKPGSNCSSAQS CCCCCCCCCCHHHHH	43.10	27097102
24	Phosphorylation	PKPGSNCSSAQSVLS CCCCCCCHHHHHHHH	32.72	27097102
25	Phosphorylation	KPGSNCSSAQSVLSE CCCCCCHHHHHHHHH	32.31	27097102
28	Phosphorylation	SNCSSAQSVLSEVSS CCCHHHHHHHHHHCC	25.41	27097102
31	Phosphorylation	SSAQSVLSEVSSVPT HHHHHHHHHHCCCCC	33.52	23984901
34	Phosphorylation	QSVLSEVSSVPTNGM HHHHHHHCCCCCCCC	22.90	23984901
35	Phosphorylation	SVLSEVSSVPTNGMA HHHHHHCCCCCCCCC	36.97	27097102
38	Phosphorylation	SEVSSVPTNGMAKNG HHHCCCCCCCCCCCC	42.10	27097102
46	Phosphorylation	NGMAKNGSEADIDES CCCCCCCCCCCCCHH	38.72	23712012
55	Phosphorylation	ADIDESLYSRQLYVL CCCCHHHHHHHHHHH	15.87	22276854
56	Phosphorylation	DIDESLYSRQLYVLG CCCHHHHHHHHHHHH	20.88	28689409
388	Phosphorylation	DLIRKLAYVAAGDLA HHHHHHHHHHHCCCH	10.89	-
465	Ubiquitination	FGSDLQEKLGKQKYF ECHHHHHHHCCCCEE	49.97	-
465	Acetylation	FGSDLQEKLGKQKYF ECHHHHHHHCCCCEE	49.97	22902405
526	Acetylation	FRPWDVTKLKSDTAA CCCCCHHHCCHHHHH	54.49	22902405
528	Succinylation	PWDVTKLKSDTAAAA CCCHHHCCHHHHHHH	48.09	-
528	Succinylation	PWDVTKLKSDTAAAA CCCHHHCCHHHHHHH	48.09	-
598	Phosphorylation	YRKPLLESGTLGTKG EECCCHHCCCCCCCC	36.73	23984901
600	Phosphorylation	KPLLESGTLGTKGNV CCCHHCCCCCCCCCE	31.15	23984901
603	Phosphorylation	LESGTLGTKGNVQVV HHCCCCCCCCCEEEE	38.60	23984901
671	Ubiquitination	NQYLTDSKFVERTLR HHHHCCHHHHHHHHH	57.13	-
671	Acetylation	NQYLTDSKFVERTLR HHHHCCHHHHHHHHH	57.13	22902405
800	Phosphorylation	SVQVPEFTPKSGVKI HCCCCCCCCCCCCEE	28.30	22108457
810	Phosphorylation	SGVKIHVSDQELQSA CCCEEEECHHHHHHC	21.29	29779826
816	Phosphorylation	VSDQELQSANASVDD ECHHHHHHCCCCCCH	35.79	27097102
820	Phosphorylation	ELQSANASVDDSRLE HHHHCCCCCCHHHHH	26.36	27097102
824	Phosphorylation	ANASVDDSRLEELKA CCCCCCHHHHHHHHH	34.01	23984901
832	Phosphorylation	RLEELKATLPSPDKL HHHHHHHHCCCCCCC	37.30	28432305
835	Phosphorylation	ELKATLPSPDKLPGF HHHHHCCCCCCCCCC	49.17	30240740
838	Ubiquitination	ATLPSPDKLPGFKMY HHCCCCCCCCCCEEE	61.02	-
838	Acetylation	ATLPSPDKLPGFKMY HHCCCCCCCCCCEEE	61.02	22902405
873	Phosphorylation	SNLRAENYDISPADR CCCCCCCCCCCHHHH	13.22	-
876	Phosphorylation	RAENYDISPADRHKS CCCCCCCCHHHHHHH	15.90	28551015
980	Acetylation	KQFLDYFKTEHKLEI HHHHHHHHHCCHHHH	46.93	-
1020	Phosphorylation	QPMTEIVSRVSKRKL CHHHHHHHHHHHHHH	32.40	23984901
1023	Phosphorylation	TEIVSRVSKRKLGRH HHHHHHHHHHHHHHH	26.15	23984901

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of UBA1_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of UBA1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UBA1_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of UBA1_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.