UB2J1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UB2J1_MOUSE
• UniProt AC: Q9JJZ4
• Protein Name: Ubiquitin-conjugating enzyme E2 J1
• Gene Name: Ube2j1
• Organism: Mus musculus (Mouse).
• Sequence Length: 318
• Subcellular Localization: Endoplasmic reticulum membrane ; Single-pass type IV membrane protein .
• Protein Description: Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD)..
• Protein Sequence: METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSGSSQADQEAKELARQISFKAEVNSSGKTIAESDLNQCFSLNDSQDDLPTTFQGATASTSYGAQNPSGAPLPQPTQPAPKNTSMSPRQRRAQQQSQRRPSTSPDVLQGQPPRAHHTEHGGSAMLIIILTLALAALIFRRIYLANEYIFDFEL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---METRYNLKSPAV ---CCCCCCCCCHHH	32.06	-
8	Ubiquitination	METRYNLKSPAVKRL CCCCCCCCCHHHHHH	51.24	27667366
8	Malonylation	METRYNLKSPAVKRL CCCCCCCCCHHHHHH	51.24	26320211
9	Phosphorylation	ETRYNLKSPAVKRLM CCCCCCCCHHHHHHH	22.66	28066266
177	Ubiquitination	SQADQEAKELARQIS CHHHHHHHHHHHHHH	53.13	27667366
184	Phosphorylation	KELARQISFKAEVNS HHHHHHHHHHEEECC	16.62	26824392
191	Phosphorylation	SFKAEVNSSGKTIAE HHHEEECCCCCEEEH	45.72	23984901
249	Phosphorylation	QPAPKNTSMSPRQRR CCCCCCCCCCHHHHH	27.02	28066266
251	Phosphorylation	APKNTSMSPRQRRAQ CCCCCCCCHHHHHHH	19.39	27149854
261	Phosphorylation	QRRAQQQSQRRPSTS HHHHHHHHHCCCCCC	22.71	25159016
266	Phosphorylation	QQSQRRPSTSPDVLQ HHHHCCCCCCHHHHC	39.44	27087446
267	Phosphorylation	QSQRRPSTSPDVLQG HHHCCCCCCHHHHCC	47.14	25521595
268	Phosphorylation	SQRRPSTSPDVLQGQ HHCCCCCCHHHHCCC	24.36	27087446

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
184	S	Phosphorylation	Kinase	MAPKAPK2	P49138	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of UB2J1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UB2J1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of UB2J1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, AND MASSSPECTROMETRY.
PubMed: 17242355

"Mitochondrial phosphoproteome revealed by an improved IMAC method andMS/MS/MS.";
Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y.;
Mol. Cell. Proteomics 6:669-676(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, AND MASSSPECTROMETRY.
PubMed: 17208939