TWF1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TWF1_MOUSE
• UniProt AC: Q91YR1
• Protein Name: Twinfilin-1
• Gene Name: Twf1
• Organism: Mus musculus (Mouse).
• Sequence Length: 350
• Subcellular Localization: Cytoplasm . Cytoplasm, cytoskeleton . Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
• Protein Description: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles..
• Protein Sequence: MSHQTGIQASEDVKEIFARARNGKYRLLKISIENEQLVVGSCSPPSDSWEQDYDSFVLPLLEDKQPCYVLFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVSVDTKHQTLQGVAFPISRDAFQALEKLSKKQLNYVQLEIDIKNETIILANTENTELRDLPKRIPKDSARYHFFLYKHSHEGDYLESVVFIYSMPGYTCSIRERMLYSSCKSPLLEIVERQLQMDVIRKIEIDNGDELTADFLYDEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAEAEATTD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSHQTGIQA ------CCCCCCCCC	25.05	-
2	Phosphorylation	------MSHQTGIQA ------CCCCCCCCC	25.05	26824392
136	Malonylation	VSLHGYKKYLLSQSS EECCCHHHHHHHCCC	32.42	26320211
136	Acetylation	VSLHGYKKYLLSQSS EECCCHHHHHHHCCC	32.42	22826441
137	Phosphorylation	SLHGYKKYLLSQSSP ECCCHHHHHHHCCCC	14.55	24925903
140	Phosphorylation	GYKKYLLSQSSPAPL CHHHHHHHCCCCCCC	25.97	24925903
142	Phosphorylation	KKYLLSQSSPAPLTA HHHHHHCCCCCCCCH	34.61	24925903
143	Phosphorylation	KYLLSQSSPAPLTAA HHHHHCCCCCCCCHH	19.90	25521595
148	Phosphorylation	QSSPAPLTAAEEELR CCCCCCCCHHHHHHH	23.82	24925903
164	Phosphorylation	IKINEVQTDVSVDTK CCCCCCCCCCCCCCC	43.47	26239621
167	Phosphorylation	NEVQTDVSVDTKHQT CCCCCCCCCCCCCCE	19.65	26239621
192	Ubiquitination	DAFQALEKLSKKQLN HHHHHHHHHCHHHCC	59.93	22790023
273	Phosphorylation	IRERMLYSSCKSPLL HHHHHHHHCCCCHHH	25.87	23984901
274	Phosphorylation	RERMLYSSCKSPLLE HHHHHHHCCCCHHHH	16.41	23984901
275	Glutathionylation	ERMLYSSCKSPLLEI HHHHHHCCCCHHHHH	4.09	24333276
277	Phosphorylation	MLYSSCKSPLLEIVE HHHHCCCCHHHHHHH	25.71	27180971
309	Phosphorylation	ELTADFLYDEVHPKQ EEEEEEECCCCCHHH	15.28	22817900
322	Phosphorylation	KQHAHKQSFAKPKGP HHHCCCHHCCCCCCC	31.79	26824392
348	Phosphorylation	GPAEAEATTD----- CCCHHCCCCC-----	24.01	27087446
349	Phosphorylation	PAEAEATTD------ CCHHCCCCC------	46.92	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TWF1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TWF1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TWF1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of TWF1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry.";
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
Mol. Cell. Proteomics 8:904-912(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-349, AND MASSSPECTROMETRY.
PubMed: 19131326

"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain.";
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
J. Proteome Res. 7:311-318(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-309, AND MASSSPECTROMETRY.
PubMed: 18034455