TRIPC_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TRIPC_RAT
• UniProt AC: F1LP64
• Protein Name: E3 ubiquitin-protein ligase TRIP12
• Gene Name: Trip12
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 2025
• Subcellular Localization: Nucleus, nucleoplasm.
• Protein Description: E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardeless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes..
• Protein Sequence: MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESLSETNKPPSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSDLKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGSGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESSKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMRDKLLIAAREGQQSFHLS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSNRPNNNP ------CCCCCCCCC		-
2	Phosphorylation	------MSNRPNNNP ------CCCCCCCCC		22668510
12	Phosphorylation	PNNNPGGSLRRSQRN CCCCCCCCCCHHHCC		27097102
34	Phosphorylation	DDSIGGRSCSSSSAV CCCCCCCCCCCCCCE		25575281
36	Phosphorylation	SIGGRSCSSSSAVIV CCCCCCCCCCCCEEE		25575281
37	Phosphorylation	IGGRSCSSSSAVIVP CCCCCCCCCCCEEEC		25575281
38	Phosphorylation	GGRSCSSSSAVIVPQ CCCCCCCCCCEEECC		22108457
39	Phosphorylation	GRSCSSSSAVIVPQP CCCCCCCCCEEECCC		25575281
75	Phosphorylation	NSRGVKRSASPDYNR CCCCCCCCCCCCCCC		28432305
77	Phosphorylation	RGVKRSASPDYNRTN CCCCCCCCCCCCCCC		29779826
80	Phosphorylation	KRSASPDYNRTNSPS CCCCCCCCCCCCCCC		28432305
83	Phosphorylation	ASPDYNRTNSPSSAK CCCCCCCCCCCCCCC		28689409
85	Phosphorylation	PDYNRTNSPSSAKKP CCCCCCCCCCCCCCC		28689409
87	Phosphorylation	YNRTNSPSSAKKPRA CCCCCCCCCCCCCCH		28689409
88	Phosphorylation	NRTNSPSSAKKPRAF CCCCCCCCCCCCCHH		25575281
100	Phosphorylation	RAFQHIESLSETNKP CHHHHHHHHHHCCCC		25575281
102	Phosphorylation	FQHIESLSETNKPPS HHHHHHHHHCCCCCC		25575281
109	Phosphorylation	SETNKPPSKSKKRHL HHCCCCCCHHHHHCC		27097102
159	Phosphorylation	TESSCVKSGSGSEST CCCCCCCCCCCCCCC		28432305
161	Phosphorylation	SSCVKSGSGSESTGA CCCCCCCCCCCCCCC		22108457
163	Phosphorylation	CVKSGSGSESTGAEE CCCCCCCCCCCCCCH		28432305
177	Acetylation	ERSAKPTKLASKSAT HHCCCCCCHHCCCCC		72593389
181	Acetylation	KPTKLASKSATSAKA CCCCHHCCCCCCCCC		-
236	Phosphorylation	NKARRSRSASSPSPR HHHHHHHCCCCCCCC		24945867
238	Phosphorylation	ARRSRSASSPSPRRS HHHHHCCCCCCCCCC		24945867
239	Phosphorylation	RRSRSASSPSPRRSS HHHHCCCCCCCCCCH		24945867
241	Phosphorylation	SRSASSPSPRRSSRE HHCCCCCCCCCCHHH		24945867
310	Phosphorylation	RKSTKKRSESPPAEL HHHCCCCCCCCCCCC		27097102
312	Phosphorylation	STKKRSESPPAELPS HCCCCCCCCCCCCCH		23712012
319	Phosphorylation	SPPAELPSLRRSTRQ CCCCCCCHHHHCCCC		27097102
340	Phosphorylation	ASTSRRGSGLGKRGA CCCCCCCCCCCHHHH		29779826
870	Phosphorylation	LAKSFIKTLFGVLYE HHHHHHHHHHHHHHH		25575281
876	Phosphorylation	KTLFGVLYEVYSSSA HHHHHHHHHHHHCCC		25575281
879	Phosphorylation	FGVLYEVYSSSAGPA HHHHHHHHHCCCCHH		25575281
880	Phosphorylation	GVLYEVYSSSAGPAV HHHHHHHHCCCCHHH		25575281
881	Phosphorylation	VLYEVYSSSAGPAVR HHHHHHHCCCCHHHH		25575281
882	Phosphorylation	LYEVYSSSAGPAVRH HHHHHHCCCCHHHHH		25575281
971	Phosphorylation	KHLAESESLLTSPPK HHHHHCHHHCCCCCC		30181290
974	Phosphorylation	AESESLLTSPPKACT HHCHHHCCCCCCCCC		28432305
975	Phosphorylation	ESESLLTSPPKACTN HCHHHCCCCCCCCCC		27097102
1020	Phosphorylation	LQHSRDDSLDLSPQG CCCCCCCCCCCCCCC		27097102
1024	Phosphorylation	RDDSLDLSPQGRLSD CCCCCCCCCCCCHHH		27097102
1030	Phosphorylation	LSPQGRLSDVLKRKR CCCCCCHHHHHHHCC		27097102
1048	Phosphorylation	RGPRRPKYSPPRDDD CCCCCCCCCCCCCCC		27097102
1049	Phosphorylation	GPRRPKYSPPRDDDK CCCCCCCCCCCCCCC		29779826
1063	Phosphorylation	KVDNQAKSPTTTQSP CCCCCCCCCCCCCCC		29779826
1065	Phosphorylation	DNQAKSPTTTQSPKS CCCCCCCCCCCCCCH		25575281
1066	Phosphorylation	NQAKSPTTTQSPKSS CCCCCCCCCCCCCHH		27097102
1067	Phosphorylation	QAKSPTTTQSPKSSF CCCCCCCCCCCCHHH		27097102
1069	Phosphorylation	KSPTTTQSPKSSFLA CCCCCCCCCCHHHHH		27097102
1087	Phosphorylation	PKTWGRLSAQSNSNN CCCHHHCCCCCCCCC		27097102
1090	Phosphorylation	WGRLSAQSNSNNIEP HHHCCCCCCCCCCCH		27097102
1092	Phosphorylation	RLSAQSNSNNIEPAR HCCCCCCCCCCCHHH		27097102
1104	Phosphorylation	PARTAGVSGLARAAS HHHHHCHHHHHHHHC		30240740
1111	Phosphorylation	SGLARAASKDTISNN HHHHHHHCCCCHHCC		28432305
1137	Phosphorylation	AHKFVERYFSSENMD HHHHHHHHHCCCCCC		27097102
1139	Phosphorylation	KFVERYFSSENMDGS HHHHHHHCCCCCCCC		27097102
1140	Phosphorylation	FVERYFSSENMDGSN HHHHHHCCCCCCCCC		27097102
1146	Phosphorylation	SSENMDGSNPALNVL CCCCCCCCCHHHHHH		27097102
1203	Phosphorylation	FVKQLLLYLTSKSEK HHHHHHHHHHCCCHH		-
1274	Phosphorylation	VKVHDFPSGNGSGGS CEEECCCCCCCCCCC		27097102
1278	Phosphorylation	DFPSGNGSGGSFSLN CCCCCCCCCCCCCCC		27097102
1281	Phosphorylation	SGNGSGGSFSLNRGS CCCCCCCCCCCCCCC		27097102
1283	Phosphorylation	NGSGGSFSLNRGSQA CCCCCCCCCCCCCCH		27097102
1292	Acetylation	NRGSQALKFFNTHQL CCCCCHHHHCCHHHH		72600949
1321	Acetylation	QWKGGPVKIDPLALV CCCCCCCCCCHHHHH		72584759
1350	Phosphorylation	VREDDEDSDDDGSDE CCCCCCCCCCCCCHH		29779826
1355	Phosphorylation	EDSDDDGSDEEIDES CCCCCCCCHHHHHHH		29779826
1362	Phosphorylation	SDEEIDESLAAQFLN CHHHHHHHHHHHHHH		22668510
1370	Phosphorylation	LAAQFLNSGNVRHRL HHHHHHHCCCHHHEE		22673903
1400	Phosphorylation	YQAVRQFSVQAEDER HHHHHHHCCCCCCCC		25575281
1409	Phosphorylation	QAEDERESTDDESNP CCCCCCCCCCCCCCC		29779826
1410	Phosphorylation	AEDERESTDDESNPL CCCCCCCCCCCCCCC		27097102
1414	Phosphorylation	RESTDDESNPLGRAG CCCCCCCCCCCCCCC		22673903
1454	Phosphorylation	GKRGRAQTAPTKTSP CCCCCCCCCCCCCCC		28432305
1457	Phosphorylation	GRAQTAPTKTSPRNA CCCCCCCCCCCCCCH		27097102
1458	Acetylation	RAQTAPTKTSPRNAK CCCCCCCCCCCCCHH		-
1459	Phosphorylation	AQTAPTKTSPRNAKK CCCCCCCCCCCCHHC		27097102
1460	Phosphorylation	QTAPTKTSPRNAKKH CCCCCCCCCCCHHCC		29779826
1540	Ubiquitination	TSEFINSKLTAKANR HHHHHHHHHHHHHHH		-
1608	Phosphorylation	TNPEINQSDSQDSRV CCCCCCCCCCCCCCH		28432305
1610	Phosphorylation	PEINQSDSQDSRVAP CCCCCCCCCCCCHHH		28432305
1613	Phosphorylation	NQSDSQDSRVAPRLD CCCCCCCCCHHHHHC		28432305
1644	Phosphorylation	SVMQDLGSSRAMLEI HHHHHHCCCCEEEEE		25575281
1645	Phosphorylation	VMQDLGSSRAMLEIQ HHHHHCCCCEEEEEE		25575281
1974	Ubiquitination	PPLTIVRKTFESTEN CCEEEEEHHHCCCCC		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TRIPC_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TRIPC_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TRIPC_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of TRIPC_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.