TPX2A_XENLA - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TPX2A_XENLA
• UniProt AC: Q6NUF4
• Protein Name: Targeting protein for Xklp2-A
• Gene Name: tpx2-a
• Organism: Xenopus laevis (African clawed frog).
• Sequence Length: 716
• Subcellular Localization: Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Localizes during metaphase on spindle microtubules, with a strong enrichment at spindle poles. Localizes to the minus ends of spindle pole and aster microtubules in a d
• Protein Description: Spindle assembly factor. Required for normal assembly of mitotic spindles. Mediates the binding kif15 and aurka to spindle microtubules. Required for targeting kif15 to microtubule minus ends. Activates aurka by promoting its autophosphorylation and protects the phosphorylated residue against dephosphorylation (By similarity)..
• Protein Sequence: MEDTQDTYSYDAPSIFNFSSFHEDHNADSWFDQVTNAENIPPDQRRLSETSVNTEQNSKVEPVQTTPSKDDVSNSATHVCDVKSQSKRSSRRMSKKHRQKLLVKMKDTHLEKETAPPEYPPCKKLKGSSSKGRHAPVIKSQSTSSHHSMTSPKPKAQLTMPSTPTVLKRRNVLVKAKNSEEQELEKMQELQKEMLENLKKNEHSMKVAITGAGQPVKTFIPVTKPVDFHFKTDDRLKRTANQPEGDGYKAVDFASELRKHPPSPVQVTKGGHTVPKPFNLSKGKRKHEEASDYVSTAEQVIAFYKRTPARYHLRSRQREMEGPSPVKMIKTKLTNPKTPLLQTKGRHRPVTCKSAAELEAEELEMINQYKFKAQELDTRILEGGPVLLKKPLVKEPTKAIGFDLEIEKRIQQREKKEEIEEETFTFHSRPCPSKMLTDVVGVPLKKLLPVTVPQSPAFALKNRVRIPAQEEKQEEMVPVIKATRMPHYGVPFKPKLVEQRQVDVCPFSFCDRDKERQLQKEKRLDELRKDEVPKFKAQPLPQFDNIRLPEKKVKMPTQQEPFDLEIEKRGASKLQRWQQQIQEELKQQKEMVVFKARPNTVVHQEPFVPKKENRSLTESLSGSIVQEGFELATAKRAKERQEFDKCLAETEAQKSLLEEEIRKRREEEEKEEISQLRQELVHKAKPIRKYRAVEVKASDVPLTVPRSPNFSDRFKC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
48	Phosphorylation	PPDQRRLSETSVNTE CCCHHHHHCCCCCCC	36.70	14701852
90	Phosphorylation	KSQSKRSSRRMSKKH CCCCHHHHHHHCHHH	28.12	14701852
94	Phosphorylation	KRSSRRMSKKHRQKL HHHHHHHCHHHHHHH	36.42	14701852
204	Phosphorylation	NLKKNEHSMKVAITG HHHHCCCCCEEEECC	17.70	19556869

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
48	S	Phosphorylation	Kinase	AURKA	O14965	GPS
48	S	Phosphorylation	Kinase	AURKA	-	PhosphoELM
90	S	Phosphorylation	Kinase	AURKA	O14965	GPS
90	S	Phosphorylation	Kinase	AURKA	-	PhosphoELM
94	S	Phosphorylation	Kinase	AURKA	O14965	GPS
94	S	Phosphorylation	Kinase	AURKA	-	PhosphoELM
204	S	Phosphorylation	Kinase	PLK1	P70032	Uniprot
204	S	Phosphorylation	Kinase	PLK1	-	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TPX2A_XENLA !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TPX2A_XENLA !!

Protein-Protein Interaction

Oops, there are no PPI records of TPX2A_XENLA !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A.";
Eckerdt F., Pascreau G., Phistry M., Lewellyn A.L.,DePaoli-Roach A.A., Maller J.L.;
Cell Cycle 8:2413-2419(2009).
Cited for: FUNCTION, PHOSPHORYLATION AT SER-204, MUTAGENESIS OF SER-204, ANDINTERACTION WITH PLK1.
PubMed: 19556869