TOP3B_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TOP3B_MOUSE
• UniProt AC: Q9Z321
• Protein Name: DNA topoisomerase 3-beta-1
• Gene Name: Top3b
• Organism: Mus musculus (Mouse).
• Sequence Length: 862
• Protein Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity..
• Protein Sequence: MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVMNNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHRVRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRGRRPPGKPNPRRPKDKMSALAAYFV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
75	Acetylation	GKYNKWDKVDPAELF CCCCCCCCCCHHHHH	47.63	23806337
87	Phosphorylation	ELFSQAPTEKKEANP HHHHCCCCCCCCCCC	64.55	22871156
351	Acetylation	YPENFDLKGSLRQQA CCCCCCCCCCHHHHC	48.58	23954790
432	Phosphorylation	KYLQSTISFRIGPEH CHHHCEEEEEECHHH	14.46	28973931
548	Phosphorylation	GIVLVHGYYKIDAEL EEEEECEEEEECHHH	6.16	-
595	Phosphorylation	IFKRKFHYFVDSIAG HHHHHHHHHHHHCCC	14.39	29109428
617	Phosphorylation	SFSPLAATGKPLSRC ECCCHHCCCCCHHHC	39.88	29109428

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TOP3B_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TOP3B_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TOP3B_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of TOP3B_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.