TOP3B_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TOP3B_DROME
• UniProt AC: O96651
• Protein Name: DNA topoisomerase 3-beta
• Gene Name: Top3beta
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 875
• Protein Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA..
• Protein Sequence: MKSVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHVMSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEGENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQELDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHLQLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTVELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFGEEARSILGDIQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDLKYRVTTAKLSVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALPIGNVKVYREFKCPLDDFDLLAFSTGVKGRSYPFCPYCYNHPPFSDMPHLGGCNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVEKHRAVASRPVRSGGGFRGGKAGRGGGGMGGAAFGSGGAVTAGGGPNAGGGVRGSRVAKDKMGQLASYFV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
32	Phosphorylation	RCTAKRGTGNGCSTH CEEEECCCCCCCCCC	31.35	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TOP3B_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TOP3B_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TOP3B_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FMR1_DROME	Fmr1	genetic	23912945
FMR1_DROME	Fmr1	physical	23912945

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.