dbPTM

TOP2A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TOP2A_MOUSE
UniProt AC	Q01320
Protein Name	DNA topoisomerase 2-alpha
Gene Name	Top2a
Organism	Mus musculus (Mouse).
Sequence Length	1528
Subcellular Localization	Nucleus.
Protein Description	Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. [PubMed: 24321095]
Protein Sequence	MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVVDLESDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MELSPLQP -------CCCCCCCC	48.18	-
4	Phosphorylation	----MELSPLQPVNE ----CCCCCCCCCCH	27.19	26824392
28	Phosphorylation	EDGKKRLSIERIYQK CCCCCHHCHHHHHCC	26.83	22942356
130	Ubiquitination	GIPVVEHKVEKIYVP CCCEEEECCEECCCC	38.29	27667366
130	Acetylation	GIPVVEHKVEKIYVP CCCEEEECCEECCCC	38.29	22826441
155	Ubiquitination	SNYDDDEKKVTGGRN CCCCCCCCCCCCCCC	60.58	-
156	Ubiquitination	NYDDDEKKVTGGRNG CCCCCCCCCCCCCCC	42.87	-
229	Phosphorylation	LSKFKMQSLDKDIVA HHHHCCCCCCHHHHH	34.73	22817900
281	Phosphorylation	LKDKVDETGNSLKVI EHHCCCCCCCCEEEE	36.88	-
327	Phosphorylation	KGGRHVDYVADQIVS CCCCCHHHHHHHHHH	9.10	28066266
391	S-nitrosocysteine	AKSFGSTCQLSEKFI EHHCCCHHHCCHHHH	4.03	-
391	S-nitrosylation	AKSFGSTCQLSEKFI EHHCCCHHHCCHHHH	4.03	20925432
394	Phosphorylation	FGSTCQLSEKFIKAA CCCHHHCCHHHHHHH	16.92	-
404	S-nitrosocysteine	FIKAAIGCGIVESIL HHHHHHHCCHHHHHH	2.48	-
404	S-nitrosylation	FIKAAIGCGIVESIL HHHHHHHCCHHHHHH	2.48	20925432
409	Phosphorylation	IGCGIVESILNWVKF HHCCHHHHHHHHHHH	22.83	22817900
448	Phosphorylation	DDANDAGSRNSTECT CCCCCCCCCCCCEEE	29.97	27841257
459	Phosphorylation	TECTLILTEGDSAKT CEEEEEEECCCCCCE	30.81	22802335
463	Phosphorylation	LILTEGDSAKTLAVS EEEECCCCCCEEEEE	42.39	22802335
479	Acetylation	LGVVGRDKYGVFPLR EECCCCCCCCCCCCC	42.41	23806337
528	Acetylation	YEDEDSLKTLRYGKI CCCHHHHHHEEECEE	49.84	22826441
580	Phosphorylation	ITPIVKVSKNKQEIA CHHHEEECCCCCEEE	25.45	23737553
601	Phosphorylation	FEEWKSSTPNHKKWK HHHHHCCCCCCCCEE	34.89	19367708
610	Acetylation	NHKKWKVKYYKGLGT CCCCEEEEEECCCCC	38.85	6571715
621	Acetylation	GLGTSTSKEAKEYFA CCCCCCCHHHHHHHH	62.88	6571661
621	Ubiquitination	GLGTSTSKEAKEYFA CCCCCCCHHHHHHHH	62.88	-
706	Phosphorylation	NKELILFSNSDNERS CCEEEEEECCCCCCC	31.79	28066266
708	Phosphorylation	ELILFSNSDNERSIP EEEEEECCCCCCCCC	40.38	28066266
797	Ubiquitination	GTRLHGGKDSASPRY CCCCCCCCCCCCHHH	53.77	-
996	S-nitrosocysteine	KLQSSLTCNSMVLFD HHCCCCCCCCEEEEC	4.27	-
996	S-nitrosylation	KLQSSLTCNSMVLFD HHCCCCCCCCEEEEC	4.27	20925432
1033	Acetylation	LKYYGLRKEWLLGML HHHHCCCHHHHHHHH	59.28	22826441
1105	Phosphorylation	DEEENEESDTETSTS CHHHCCCCCCCCCCC	43.59	21149613
1107	Phosphorylation	EENEESDTETSTSDS HHCCCCCCCCCCCHH	50.90	21149613
1109	Phosphorylation	NEESDTETSTSDSAA CCCCCCCCCCCHHHH	39.12	21149613
1110	Phosphorylation	EESDTETSTSDSAAE CCCCCCCCCCHHHHH	21.49	21149613
1111	Phosphorylation	ESDTETSTSDSAAEA CCCCCCCCCHHHHHH	43.93	25159016
1112	Phosphorylation	SDTETSTSDSAAEAG CCCCCCCCHHHHHHC	29.36	22345495
1114	Phosphorylation	TETSTSDSAAEAGPT CCCCCCHHHHHHCCC	29.19	22802335
1121	Phosphorylation	SAAEAGPTFNYLLDM HHHHHCCCHHHHHHC	24.48	22345495
1124	Phosphorylation	EAGPTFNYLLDMPLW HHCCCHHHHHHCCHH	12.40	22345495
1132	Phosphorylation	LLDMPLWYLTKEKKD HHHCCHHHHCHHHHH	16.51	22345495
1134	Phosphorylation	DMPLWYLTKEKKDEL HCCHHHHCHHHHHHH	22.82	22345495
1159	Phosphorylation	LNTLKQKSPSDLWKE HHHHHHCCHHHHHHH	27.43	28066266
1161	Phosphorylation	TLKQKSPSDLWKEDL HHHHCCHHHHHHHHH	53.04	28066266
1204	Oxidation	AKGKKAQMCADVLPS CCCCCCCCHHHCCCC	2.13	17242355
1211	Phosphorylation	MCADVLPSPRGKRVI CHHHCCCCCCCCCCC	24.06	27087446
1238	Sumoylation	KKIRKKIKSENVEGT HHHHHHHHHCCCCCC	61.67	28289178
1239	Phosphorylation	KIRKKIKSENVEGTP HHHHHHHHCCCCCCC	37.57	25619855
1245	Phosphorylation	KSENVEGTPAEDGAE HHCCCCCCCCCCCCC	13.06	26824392
1255	Phosphorylation	EDGAEPGSLRQRIEK CCCCCCCHHHHHHHH	30.97	25619855
1291	Phosphorylation	GRKKNPWSDSESDVS CCCCCCCCCCCCCCC	32.30	25159016
1293	Phosphorylation	KKNPWSDSESDVSSN CCCCCCCCCCCCCCC	33.22	25159016
1295	Phosphorylation	NPWSDSESDVSSNES CCCCCCCCCCCCCCC	47.46	25159016
1298	Phosphorylation	SDSESDVSSNESNVD CCCCCCCCCCCCCCC	32.22	25159016
1299	Phosphorylation	DSESDVSSNESNVDV CCCCCCCCCCCCCCC	44.45	25159016
1302	Phosphorylation	SDVSSNESNVDVPPR CCCCCCCCCCCCCHH	46.53	25159016
1311	Acetylation	VDVPPRQKEQRSAAA CCCCHHHHHHHHHHH	58.03	23864654
1319	Acetylation	EQRSAAAKAKFTVDL HHHHHHHHCCEEEEC	47.59	23864654
1323	Phosphorylation	AAAKAKFTVDLDSDE HHHHCCEEEECCCCC	16.65	21082442
1328	Phosphorylation	KFTVDLDSDEDFSGL CEEEECCCCCCCCCC	50.63	21082442
1333	Phosphorylation	LDSDEDFSGLDEKDE CCCCCCCCCCCCCCC	51.25	21082442
1350	Phosphorylation	DFLPLDATPPKAKIP CCCCCCCCCCCCCCC	39.53	25521595
1367	Phosphorylation	NTKKALKTQGSSMSV CHHHHHHHCCCCCEE	38.73	21149613
1370	Phosphorylation	KALKTQGSSMSVVDL HHHHHCCCCCEEEEC	17.04	21082442
1371	Phosphorylation	ALKTQGSSMSVVDLE HHHHCCCCCEEEECC	23.15	22942356
1373	Phosphorylation	KTQGSSMSVVDLESD HHCCCCCEEEECCCH	22.42	21082442
1379	Phosphorylation	MSVVDLESDVKDSVP CEEEECCCHHCCCCC	55.81	25159016
1384	Phosphorylation	LESDVKDSVPASPGV CCCHHCCCCCCCCCC	25.04	22942356
1388	Phosphorylation	VKDSVPASPGVPAAD HCCCCCCCCCCCHHH	19.00	27087446
1400	Phosphorylation	AADFPAETEQSKPSK HHHCCCCCCCCCCCC	41.24	25619855
1403	Phosphorylation	FPAETEQSKPSKKTV CCCCCCCCCCCCCEE	40.06	25619855
1406	Phosphorylation	ETEQSKPSKKTVGVK CCCCCCCCCCEECEE	50.99	25619855
1418	Acetylation	GVKKTATKSQSSVST CEEECCCCCCCCCCC	43.99	23806337
1419	Phosphorylation	VKKTATKSQSSVSTA EEECCCCCCCCCCCC	30.82	27841257
1421	Phosphorylation	KTATKSQSSVSTAGT ECCCCCCCCCCCCCC	39.54	27149854
1422	Phosphorylation	TATKSQSSVSTAGTK CCCCCCCCCCCCCCC	16.67	27841257
1424	Phosphorylation	TKSQSSVSTAGTKKR CCCCCCCCCCCCCCC	17.94	25890499
1437	Phosphorylation	KRAAPKGTKSDSALS CCCCCCCCCCCCHHH	33.24	28066266
1438	Acetylation	RAAPKGTKSDSALSA CCCCCCCCCCCHHHH	62.02	23806337
1439	Phosphorylation	AAPKGTKSDSALSAR CCCCCCCCCCHHHHH	36.31	22817900
1441	Phosphorylation	PKGTKSDSALSARVS CCCCCCCCHHHHHHC	37.89	28066266
1444	Phosphorylation	TKSDSALSARVSEKP CCCCCHHHHHHCCCC	17.47	26239621
1448	Phosphorylation	SALSARVSEKPAPAK CHHHHHHCCCCCCCC	34.06	26239621
1450	Acetylation	LSARVSEKPAPAKAK HHHHHCCCCCCCCCC	38.69	23806337
1465	Phosphorylation	NSRKRKPSSSDSSDS CCCCCCCCCCCCCHH	45.57	23375375
1466	Phosphorylation	SRKRKPSSSDSSDSD CCCCCCCCCCCCHHH	46.84	21149613
1467	Phosphorylation	RKRKPSSSDSSDSDF CCCCCCCCCCCHHHH	45.94	21149613
1469	Phosphorylation	RKPSSSDSSDSDFER CCCCCCCCCHHHHHH	37.68	21149613
1470	Phosphorylation	KPSSSDSSDSDFERA CCCCCCCCHHHHHHH	46.23	21149613
1472	Phosphorylation	SSSDSSDSDFERAIS CCCCCCHHHHHHHHH	46.26	27149854
1501	Phosphorylation	FPVDLEDTIAPRAKS CCCCHHHCCCCCCCC	15.06	25619855
1517	Phosphorylation	RARKPIKYLEESDDD CCCCCCCCCCCCCCC	21.77	25619855
1521	Phosphorylation	PIKYLEESDDDDDLF CCCCCCCCCCCCCCC	36.64	27087446

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1105	S	Phosphorylation	Kinase	CK1	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
1105	S	Phosphorylation		-
However, phosphorylation at Ser-1105 by CSNK1D/CK1 promotes DNA cleavable complex formation (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TOP2A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of TOP2A_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TOP2A_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"The phagosomal proteome in interferon-gamma-activated macrophages."; Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.; Immunity 30:143-154(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, AND MASSSPECTROMETRY.	19144319 [Abstract]
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1333, AND MASSSPECTROMETRY.	19367708 [Abstract]
"A differential phosphoproteomic analysis of retinoic acid-treated P19cells."; Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.; J. Proteome Res. 6:3174-3186(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1367; SER-1370;SER-1371; SER-1373; SER-1379; SER-1465; SER-1466; SER-1467; SER-1469;SER-1470 AND SER-1472, AND MASS SPECTROMETRY.	17622165 [Abstract]

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