TOP1M_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TOP1M_HUMAN
• UniProt AC: Q969P6
• Protein Name: DNA topoisomerase I, mitochondrial
• Gene Name: TOP1MT
• Organism: Homo sapiens (Human).
• Sequence Length: 601
• Subcellular Localization: Mitochondrion .
• Protein Description: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity)..
• Protein Sequence: MRVVRLLRLRAALTLLGEVPRRPASRGVPGSRRTQKGSGARWEKEKHEDGVKWRQLEHKGPYFAPPYEPLPDGVRFFYEGRPVRLSVAAEEVATFYGRMLDHEYTTKEVFRKNFFNDWRKEMAVEEREVIKSLDKCDFTEIHRYFVDKAAARKVLSREEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQYRADWKSREMKTRQRAVALYFIDKLALRAGNEKEDGEAADTVGCCSLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMENKDPRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPVEKIYSKTQRERFAWALAMAGEDFEF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
31	Phosphorylation	ASRGVPGSRRTQKGS HHCCCCCCCCCCCCC	16.72	23312004
34	O-linked_Glycosylation	GVPGSRRTQKGSGAR CCCCCCCCCCCCCCC	33.26	30379171
46	Acetylation	GARWEKEKHEDGVKW CCCCCHHCCCCCCCC	64.46	19823717
52	Acetylation	EKHEDGVKWRQLEHK HCCCCCCCCCCCCCC	42.03	19823725
96	Phosphorylation	AEEVATFYGRMLDHE HHHHHHHHHHHHCCC	10.24	-
104	Phosphorylation	GRMLDHEYTTKEVFR HHHHCCCCCHHHHHH	19.11	-
112	Acetylation	TTKEVFRKNFFNDWR CHHHHHHHHCCHHHH	46.79	12432645
210	Methylation	HPKMGMLKRRITPED CCCCCCEECCCCHHH	30.64	-
214	Phosphorylation	GMLKRRITPEDVVIN CCEECCCCHHHEEEE	20.86	25599653
226	Phosphorylation	VINCSRDSKIPEPPA EEECCCCCCCCCCCC	31.23	25599653
242	Phosphorylation	HQWKEVRSDNTVTWL CCCEEECCCCCCHHH	40.15	20068231
245	Phosphorylation	KEVRSDNTVTWLAAW EEECCCCCCHHHHHH	25.05	20068231
247	Phosphorylation	VRSDNTVTWLAAWTE ECCCCCCHHHHHHHH	16.84	20068231
253	Phosphorylation	VTWLAAWTESVQNSI CHHHHHHHHHHHHHH	17.41	20068231
255	Phosphorylation	WLAAWTESVQNSIKY HHHHHHHHHHHHHHH	22.94	20068231
259	Phosphorylation	WTESVQNSIKYIMLN HHHHHHHHHHHHEEC	11.90	20068231
262	Phosphorylation	SVQNSIKYIMLNPCS HHHHHHHHHEECCHH	7.01	19835603
269	Phosphorylation	YIMLNPCSKLKGETA HHEECCHHHCCCCHH	42.13	24114839
282	Phosphorylation	TAWQKFETARRLRGF HHHHHHHHHHHHHHH	28.70	24719451
295	Phosphorylation	GFVDEIRSQYRADWK HHHHHHHHHHHHHHH	36.91	19369195
316	Phosphorylation	RQRAVALYFIDKLAL HHHHHHHHHHHHHHH	6.69	28152594
320	Ubiquitination	VALYFIDKLALRAGN HHHHHHHHHHHHCCC	30.73	-
329	Ubiquitination	ALRAGNEKEDGEAAD HHHCCCCCCCCCCCC	66.17	-
384	Phosphorylation	VPVEKPVYKNLQLFM CCCCCHHHHCHHHHH	11.59	-
405	Phosphorylation	DDLFDRLTTTSLNKH CCHHHHHHHHHHHHH	28.99	-
408	Phosphorylation	FDRLTTTSLNKHLQE HHHHHHHHHHHHHHH	28.05	24719451
421	Phosphorylation	QELMDGLTAKVFRTY HHHHHHHHHHHHHHH	29.93	24719451
427	Phosphorylation	LTAKVFRTYNASITL HHHHHHHHHCCCCCH	15.06	-
428	Phosphorylation	TAKVFRTYNASITLQ HHHHHHHHCCCCCHH	12.41	-
479	Phosphorylation	TPSTFEKSMQNLQTK CHHHHHHHHHHHHHH	20.57	28842319
485	Phosphorylation	KSMQNLQTKIQAKKE HHHHHHHHHHHHHHH	33.03	29888752
515	Acetylation	HKAQGDGKSRSVLEK HHHCCCCCCHHHHHH	47.65	7710503
559	Phosphorylation	LGTSKLNYLDPRISI EECCCCCCCCCCCEE	23.95	28152594

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TOP1M_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TOP1M_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TOP1M_HUMAN !!

Protein-Protein Interaction

Oops, there are no PPI records of TOP1M_HUMAN !!

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00762: Irinotecan
• DB01030: Topotecan