TOP1A_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TOP1A_ARATH
• UniProt AC: P30181
• Protein Name: DNA topoisomerase 1 alpha {ECO:0000305}
• Gene Name: TOP1A {ECO:0000305}
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 916
• Subcellular Localization: Nucleus .
• Protein Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Can complement a TOP1-deficient yeast mutant. [PubMed: 16669064 Plays a critical role in the maintenance of a regular pattern of organ initiation. Topoisomerases I enzymes (TOP1A and TOP1B) are essential for plant survival]
• Protein Sequence: MGTETVSKPVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMRSPVTSPNGTTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQLQKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNEVPNGKVLGKRPLEKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFTKSLLEKFAWAMDVEPEYRFSRR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15	Phosphorylation	KPVMDNGSGDSDDDK CCCCCCCCCCCCCCC		27531888
18	Phosphorylation	MDNGSGDSDDDKPLA CCCCCCCCCCCCCCC		27531888
55	Phosphorylation	AVPTTKVSPMRSPVT CCCCCCCCCCCCCCC		30589143
88	Phosphorylation	PSSSSKASPAKSPLR CCCCCCCCCCCCCCC		25561503
92	Phosphorylation	SKASPAKSPLRNDMP CCCCCCCCCCCCCCC		30589143
113	Phosphorylation	SQLQKDQSECKIEHE HHHHHCHHHCCCCCC		23776212
122	Phosphorylation	CKIEHEDSEDDRPLS CCCCCCCCCCCCCHH		23776212
129	Phosphorylation	SEDDRPLSSILSGNK CCCCCCHHHHHCCCC		23776212
130	Phosphorylation	EDDRPLSSILSGNKG CCCCCHHHHHCCCCC		23776212
133	Phosphorylation	RPLSSILSGNKGPTS CCHHHHHCCCCCCCC		23776212
145	Phosphorylation	PTSSRQVSSPQPEKK CCCCCCCCCCCCCCC		25561503
146	Phosphorylation	TSSRQVSSPQPEKKN CCCCCCCCCCCCCCC		25561503
170	Phosphorylation	SRIIKDESDDETPIS HHHHCCCCCCCCCCH		30291188
174	Phosphorylation	KDESDDETPISSMFR CCCCCCCCCCHHHHH		23776212
177	Phosphorylation	SDDETPISSMFRKKI CCCCCCCHHHHHHHH		23776212
178	Phosphorylation	DDETPISSMFRKKID CCCCCCHHHHHHHHC		23776212
247	Phosphorylation	KKAKISASPTSVKMK HHHCCCCCCCCCCCC		23111157
249	Phosphorylation	AKISASPTSVKMKQD HCCCCCCCCCCCCHH		19880383
250	Phosphorylation	KISASPTSVKMKQDS CCCCCCCCCCCCHHH		19880383
280	Phosphorylation	KMKAKQLSTREDGTD CCCHHHCCCCCCCCC		23776212
281	Phosphorylation	MKAKQLSTREDGTDD CCHHHCCCCCCCCCC		23776212
286	Phosphorylation	LSTREDGTDDDDDDD CCCCCCCCCCCCCCC		30291188
297	Phosphorylation	DDDDVPISKRFKSDS CCCCCCHHHHHCCCC		19376835
823	Phosphorylation	GKPPLEGSDGKKIRS CCCCCCCCCCCEEEC		25561503

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TOP1A_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TOP1A_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TOP1A_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.