TLK2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TLK2_MOUSE
• UniProt AC: O55047
• Protein Name: Serine/threonine-protein kinase tousled-like 2
• Gene Name: Tlk2
• Organism: Mus musculus (Mouse).
• Sequence Length: 718
• Subcellular Localization: Nucleus . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton . Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2.
• Protein Description: Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity).; Testis-specific isoforms may play a role in spermatogenesis. Highly expressed in embryos throughout development..
• Protein Sequence: MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYDTSQGKGTPRGHKISDYFERRAEQPLYGLDGSAAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
26	Phosphorylation	RFTGVGVSKGPLNSE HHCCCCCCCCCCCCC	26.43	26824392
32	Phosphorylation	VSKGPLNSESSNQSL CCCCCCCCCCCCCCC	46.68	25293948
34	Phosphorylation	KGPLNSESSNQSLCS CCCCCCCCCCCCCCC	34.12	21149613
35	Phosphorylation	GPLNSESSNQSLCSV CCCCCCCCCCCCCCC	34.53	21149613
38	Phosphorylation	NSESSNQSLCSVGSL CCCCCCCCCCCCCCC	35.28	21149613
41	Phosphorylation	SSNQSLCSVGSLSDK CCCCCCCCCCCCCHH	34.50	21149613
44	Phosphorylation	QSLCSVGSLSDKEVE CCCCCCCCCCHHCCC	23.65	21149613
46	Phosphorylation	LCSVGSLSDKEVETP CCCCCCCCHHCCCCC	48.62	21149613
52	Phosphorylation	LSDKEVETPEKKQND CCHHCCCCCHHHHHH	41.76	21149613
70	Phosphorylation	RKRKAEPYDTSQGKG HHHHCCCCCCCCCCC	24.29	28066266
72	Phosphorylation	RKAEPYDTSQGKGTP HHCCCCCCCCCCCCC	19.67	28066266
73	Phosphorylation	KAEPYDTSQGKGTPR HCCCCCCCCCCCCCC	33.75	25266776
78	Phosphorylation	DTSQGKGTPRGHKIS CCCCCCCCCCCCCCH	17.19	28066266
85 (in isoform 2)	Phosphorylation	-	30.94	28285833
94 (in isoform 2)	Phosphorylation	-	32.11	26239621
97	Phosphorylation	RRAEQPLYGLDGSAA HHCCCCCCCCCHHHH	23.48	28285833
98 (in isoform 2)	Phosphorylation	-	25.60	23684622
99 (in isoform 2)	Phosphorylation	-	4.21	25521595
102	Phosphorylation	PLYGLDGSAAKEASE CCCCCCHHHHHHHCH	25.39	25266776
108	Phosphorylation	GSAAKEASEEQSALP HHHHHHHCHHHCHHH	42.64	25266776
110 (in isoform 2)	Phosphorylation	-	47.54	27818261
111 (in isoform 2)	Phosphorylation	-	29.92	22942356
112	Phosphorylation	KEASEEQSALPTLMS HHHCHHHCHHHHHHH	34.74	28066266
115 (in isoform 2)	Phosphorylation	-	24.17	22942356
117 (in isoform 2)	Phosphorylation	-	1.97	27566939
134 (in isoform 2)	Phosphorylation	-	43.78	29514104
145	Phosphorylation	GLCFTFVSAQQNSPS CEEEEEEEECCCCCC	19.03	25293948
150	Phosphorylation	FVSAQQNSPSSTGSG EEEECCCCCCCCCCC	23.09	25293948
152	Phosphorylation	SAQQNSPSSTGSGNT EECCCCCCCCCCCCC	40.11	25293948
153	Phosphorylation	AQQNSPSSTGSGNTE ECCCCCCCCCCCCCC	39.92	25293948
154	Phosphorylation	QQNSPSSTGSGNTEH CCCCCCCCCCCCCCC	39.44	25293948
156	Phosphorylation	NSPSSTGSGNTEHSC CCCCCCCCCCCCCCC	29.02	25293948
159	Phosphorylation	SSTGSGNTEHSCSSQ CCCCCCCCCCCCCCC	38.30	25293948
162	Phosphorylation	GSGNTEHSCSSQKQI CCCCCCCCCCCCCEE	14.95	25293948
164	Phosphorylation	GNTEHSCSSQKQISI CCCCCCCCCCCEEEE	38.53	25293948
165	Phosphorylation	NTEHSCSSQKQISIQ CCCCCCCCCCEEEEE	45.80	25293948
191	Phosphorylation	KISALENSKNSDLEK HHHHHHHCCCCHHHH	24.68	24759943
239	Acetylation	NRCVTMSKKLLIEKS HHHHHHHHHHHHHHH	36.30	7713235
240	Acetylation	RCVTMSKKLLIEKSK HHHHHHHHHHHHHHH	41.02	7713247
257	Phosphorylation	KMACRDKSMQDRLRL HHHCCCCCHHHHHHH	26.43	-
605	Phosphorylation	GKEPPKISNKVDVWS CCCCCCCCCCCCHHH	36.40	-
694	Phosphorylation	LLPHIRKSVSTSSPA HHHHHHHHCCCCCCC	16.08	25619855
696	Phosphorylation	PHIRKSVSTSSPAGA HHHHHHCCCCCCCCC	29.66	25619855
697	Phosphorylation	HIRKSVSTSSPAGAA HHHHHCCCCCCCCCH	30.94	25619855
698	Phosphorylation	IRKSVSTSSPAGAAI HHHHCCCCCCCCCHH	27.15	25619855
699	Phosphorylation	RKSVSTSSPAGAAIA HHHCCCCCCCCCHHH	20.89	25619855
707	Phosphorylation	PAGAAIASTSGASNN CCCCHHHCCCCCCCC	19.59	25293948
708	Phosphorylation	AGAAIASTSGASNNS CCCHHHCCCCCCCCC	23.35	25293948
709	Phosphorylation	GAAIASTSGASNNSS CCHHHCCCCCCCCCC	29.84	25293948
712	Phosphorylation	IASTSGASNNSSSN- HHCCCCCCCCCCCC-	39.82	25619855
715	Phosphorylation	TSGASNNSSSN---- CCCCCCCCCCC----	39.12	25619855
716	Phosphorylation	SGASNNSSSN----- CCCCCCCCCC-----	35.70	25619855
717	Phosphorylation	GASNNSSSN------ CCCCCCCCC------	46.51	25619855

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
696	S	Phosphorylation	Kinase	CHEK1	O35280	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
696	S	Phosphorylation	Phosphorylated at Ser-696, probably by CHEK1.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TLK2_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of TLK2_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.