THOC1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: THOC1_MOUSE
• UniProt AC: Q8R3N6
• Protein Name: THO complex subunit 1
• Gene Name: Thoc1
• Organism: Mus musculus (Mouse).
• Sequence Length: 657
• Subcellular Localization: Nucleus speckle. Nucleus, nucleoplasm . Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosi
• Protein Description: Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation (By similarity).; Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1 (By similarity). Essential for early embryonic development. Required for normal gene expression during postnatal testis development..
• Protein Sequence: MSPTPALFSLPEARTRFTKSTREALNNKNIKPLLTAFSQLPGSENEKKCTLDQAFRGVLEEEIINHSACENVLAIISLAIGGVTESVCTASTPFVLLGDVLDCLPLDQCDTIFTFVEKNVATWKSNTFYSAGKNYLLRMCNDLLRRLSKSQNTVFCGRIQLFLARLFPLSEKSGLNLQSQFNLENVTVFNTNEQESTLGQKHTEDREEGMDVEEGEMGDDEAPTTCSIPIDYNLYRKFWSLQDYFRNPVQCYEKISWKTFLKYSEEVLAVFKSYKLDDTQASRKKMEELKTGGEHVYFAKFLTSEKLMDLQLSDSNFRRHILLQYLILFQYLKGQVKFKSSNYVLTDEQSLWIEDTTKSVYQLLSENPPDGERFSKMVEHILNTEENWNSWKNEGCPSFVKERASDTKPTRVVRKRAAPEDFLGKGPNKKILIGNEELTRLWNLCPDNMEACKSETREYMPTLEEFFEEAIEQADPENMVESEYKAVNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIKTGEDEDEEDNDALLKENESPDVRRDKPITGEQIESFANKLGEQWKILAPYLEIKDSDIRQIECDSEDMKMRAKQLLVAWQDQEGVHATTDNLISALNKSGLSDLAESLTNDTETNS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MSPTPALF -------CCCCHHHH	11.05	-
2	Phosphorylation	------MSPTPALFS ------CCCCHHHHC	37.16	27566939
4	Phosphorylation	----MSPTPALFSLP ----CCCCHHHHCCH	17.56	26745281
9	Phosphorylation	SPTPALFSLPEARTR CCCHHHHCCHHHHHH	43.81	-
133	Acetylation	NTFYSAGKNYLLRMC CCHHHCCHHHHHHHH	41.98	-
140	S-palmitoylation	KNYLLRMCNDLLRRL HHHHHHHHHHHHHHH	2.64	28680068
300	Acetylation	GEHVYFAKFLTSEKL CCEEEEEEHHHCHHH	30.89	-
537	Phosphorylation	AKELPPPSEEIKTGE HHCCCCCHHHCCCCC	54.35	25619855
542	Phosphorylation	PPSEEIKTGEDEDEE CCHHHCCCCCCCCHH	52.01	25619855
560	Phosphorylation	ALLKENESPDVRRDK HHHHCCCCCCCCCCC	37.67	25521595
595	Ubiquitination	LAPYLEIKDSDIRQI HEEEEEECHHCCCEE	41.63	22790023
648	Phosphorylation	GLSDLAESLTNDTET CHHHHHHHHHCCCCC	34.58	25168779
650	Phosphorylation	SDLAESLTNDTETNS HHHHHHHHCCCCCCC	40.09	21743459
653	Phosphorylation	AESLTNDTETNS--- HHHHHCCCCCCC---	46.94	21743459
655	Phosphorylation	SLTNDTETNS----- HHHCCCCCCC-----	42.74	21743459
657	Phosphorylation	TNDTETNS------- HCCCCCCC-------	49.36	21743459

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of THOC1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of THOC1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of THOC1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of THOC1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.