dbPTM

TEC_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TEC_MOUSE
UniProt AC	P24604
Protein Name	Tyrosine-protein kinase Tec
Gene Name	Tec
Organism	Mus musculus (Mouse).
Sequence Length	630
Subcellular Localization	Cytoplasm. Cell membrane Peripheral membrane protein. Cytoplasm, cytoskeleton. Following B-cell or T-cell receptors activation by antigen, translocates to the plasma membrane through its PH domain. Thrombin and integrin engagement induces translocat
Protein Description	Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskeleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-82'. May also be involved in the regulation of osteoclast differentiation..
Protein Sequence	MNFNTILEEILIKRSQQKKKTSLLNYKERLCVLPKSVLSYYEGRAEKKYRKGVIDISKIKCVEIVKNDDGVIPCQNKFPFQVVHDANTLYIFAPSPQSRDRWVKKLKEEIKNNNNIMIKYHPKFWADGSYQCCRQTEKLAPGCEKYNLFESSIRKTLPPAPEIKKRRPPPPIPPEEENTEEIVVAMYDFQATEAHDLRLERGQEYIILEKNDLHWWRARDKYGSEGYIPSNYVTGKKSNNLDQYEWYCRNTNRSKAEQLLRTEDKEGGFMVRDSSQPGLYTVSLYTKFGGEGSSGFRHYHIKETATSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSTKGKNAPTTAGFSYDKWEINPSELTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLTHPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDMLLSMCQDVCEGMEYLERNSFIHRDLAARNCLVNEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEIFTEGRMPFEKNTNYEVVTMVTRGHRLHRPKLATKYLYEVMLRCWQERPEGRPSLEDLLRTIDELVECEETFGR

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
151	Phosphorylation	EKYNLFESSIRKTLP HHHCCCHHHHHHHCC	24.59	26102028
152	Phosphorylation	KYNLFESSIRKTLPP HHCCCHHHHHHHCCC	21.15	29176673
205	Phosphorylation	RLERGQEYIILEKND CCCCCCEEEEEECCC	5.83	-
227	Phosphorylation	DKYGSEGYIPSNYVT CCCCCCCCCCCCCCC	12.95	-
310	Acetylation	ETATSPKKYYLAEKH ECCCCCCCEEHHHHH	42.38	-
518	Phosphorylation	RYVLDDQYTSSSGAK EEEECCCCCCCCCCC	18.65	9473212
519	Phosphorylation	YVLDDQYTSSSGAKF EEECCCCCCCCCCCC	19.25	26745281
520	Phosphorylation	VLDDQYTSSSGAKFP EECCCCCCCCCCCCC	19.99	30635358
521	Phosphorylation	LDDQYTSSSGAKFPV ECCCCCCCCCCCCCC	25.62	30635358
522	Phosphorylation	DDQYTSSSGAKFPVK CCCCCCCCCCCCCCC	42.40	30635358
571	Phosphorylation	PFEKNTNYEVVTMVT CCCCCCCCEEEEEEE	14.20	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
518	Y	Phosphorylation	Kinase	JAK2	Q62120	Uniprot
518	Y	Phosphorylation	Kinase	LYN	P25911	Uniprot
518	Y	Phosphorylation	Kinase	TEC	P24604	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of TEC_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TEC_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
DOK1_MOUSE	Dok1	physical	10823839
PLCG1_MOUSE	Plcg1	physical	10823839

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TEC_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative time-resolved phosphoproteomic analysis of mast cellsignaling."; Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y.,Kawakami T., Salomon A.R.; J. Immunol. 179:5864-5876(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-518, AND MASSSPECTROMETRY.	17947660 [Abstract]
"Tec and Jak2 kinases cooperate to mediate cytokine-driven activationof c-fos transcription."; Yamashita Y., Watanabe S., Miyazato A., Ohya K., Ikeda U., Shimada K.,Komatsu N., Hatake K., Miura Y., Ozawa K., Mano H.; Blood 91:1496-1507(1998). Cited for: FUNCTION, PHOSPHORYLATION OF JAK2, AND PHOSPHORYLATION AT TYR-518.	9473212 [Abstract]