TBX5_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TBX5_MOUSE
• UniProt AC: P70326
• Protein Name: T-box transcription factor TBX5
• Gene Name: Tbx5
• Organism: Mus musculus (Mouse).
• Sequence Length: 518
• Subcellular Localization: Nucleus . Cytoplasm . Shuttles between the cytoplasm and the nucleus. Acetylation at Lys-339 promotes nuclear retention.
• Protein Description: DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Binds to the core DNA motif of NPPA promoter..
• Protein Sequence: MADTDEGFGLARTPLEPDSKDRSCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDLELHRMSRMQSKEYPVVPRSTVRHKVTSNHSPFSSETRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLAQEHSQIYHCTKRKDEECSSTEHPYKKPYMETSPSEEDTFYRSGYPQQQGLSTSYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGGLQPPEFLYTHGVPRTLSPHQYHSVHGVGMVPEWSENS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
252	Phosphorylation	HRMSRMQSKEYPVVP HHHHHCCCCCCCCCC	20.36	22871156
255	Phosphorylation	SRMQSKEYPVVPRST HHCCCCCCCCCCHHH	12.69	22871156
261	Phosphorylation	EYPVVPRSTVRHKVT CCCCCCHHHHEEEEC	25.78	22817900
262	Phosphorylation	YPVVPRSTVRHKVTS CCCCCHHHHEEEECC	23.88	22817900
272	Phosphorylation	HKVTSNHSPFSSETR EEECCCCCCCCCCCE	31.71	-
275	Phosphorylation	TSNHSPFSSETRALS CCCCCCCCCCCEECC	30.56	21454597
276	Phosphorylation	SNHSPFSSETRALST CCCCCCCCCCEECCC	43.28	22817900
278	Phosphorylation	HSPFSSETRALSTSS CCCCCCCCEECCCCC	24.16	22817900
339	Acetylation	SSTEHPYKKPYMETS CCCCCCCCCCCCCCC	52.29	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TBX5_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
339	K	Acetylation	Acetylation at Lys-339 by KAT2A and KAT2B promotes nuclear retention.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TBX5_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SMCA4_MOUSE	Smarca4	physical	15525990
UBC_MOUSE	Ubc	physical	25725482

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-278, AND MASSSPECTROMETRY.
PubMed: 17242355