TBX21_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TBX21_MOUSE
• UniProt AC: Q9JKD8
• Protein Name: T-box transcription factor TBX21
• Gene Name: Tbx21
• Organism: Mus musculus (Mouse).
• Sequence Length: 530
• Subcellular Localization: Nucleus .
• Protein Description: Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs. Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation. [PubMed: 10761931]
• Protein Sequence: MGIVEPGCGDMLTGTEPMPSDEGRGPGADQQHRFFYPEPGAQDPTDRRAGSSLGTPYSGGALVPAAPGRFLGSFAYPPRAQVAGFPGPGEFFPPPAGAEGYPPVDGYPAPDPRAGLYPGPREDYALPAGLEVSGKLRVALSNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACSASNTHVFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENFESMYASVDTSVPSPPGPNCQLLGGDPFSPLLSNQYPVPSRFYPDLPGQPKDMISQPYWLGTPREHSYEAEFRAVSMKPTLLPSAPGPTVPYYRGQDVLAPGAGWPVAPQYPPKMSPAGWFRPMRTLPMDPGLGSSEEQGSSPSLWPEVTSLQPEPSDSGLGEGDTKRRRISPYPSSGDSSSPAGAPSPFDKETEGQFYNYFPN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
51	Phosphorylation	PTDRRAGSSLGTPYS CCCCCCCCCCCCCCC	22.77	27566939
52	Phosphorylation	TDRRAGSSLGTPYSG CCCCCCCCCCCCCCC	29.87	28424242
55	Phosphorylation	RAGSSLGTPYSGGAL CCCCCCCCCCCCCCC	25.09	28424242
76	Phosphorylation	RFLGSFAYPPRAQVA CCCCEECCCCCHHCC	16.26	28424242
117	Phosphorylation	PDPRAGLYPGPREDY CCCCCCCCCCCHHHC	12.77	28424242
212	Phosphorylation	QCGKAEGSMPGNRLY ECCEEECCCCCCEEE	17.20	-
219	Phosphorylation	SMPGNRLYVHPDSPN CCCCCEEEECCCCCC	8.09	21690296
224	Phosphorylation	RLYVHPDSPNTGAHW EEEECCCCCCCCCCC	25.22	28424242
265	Phosphorylation	VLQSLHKYQPRLHIV HHHHHHHCCCEEEEE	17.01	21690296
302	Phosphorylation	ETQFIAVTAYQNAEI CCEEEEEEEECCCEE	15.60	23616576
304	Phosphorylation	QFIAVTAYQNAEITQ EEEEEEEECCCEEEE	7.92	21690296
313	Ubiquitination	NAEITQLKIDNNPFA CCEEEEEEECCCHHH	37.89	PubMed
498	Phosphorylation	DTKRRRISPYPSSGD CCCCEECCCCCCCCC	19.30	28285833
500	Phosphorylation	KRRRISPYPSSGDSS CCEECCCCCCCCCCC	14.68	28285833
502	Phosphorylation	RRISPYPSSGDSSSP EECCCCCCCCCCCCC	40.90	-
506	Phosphorylation	PYPSSGDSSSPAGAP CCCCCCCCCCCCCCC	35.92	28285833
507	Phosphorylation	YPSSGDSSSPAGAPS CCCCCCCCCCCCCCC	45.11	28285833
508	Phosphorylation	PSSGDSSSPAGAPSP CCCCCCCCCCCCCCC	23.79	25266776
514	Phosphorylation	SSPAGAPSPFDKETE CCCCCCCCCCCCCCC	37.35	25266776
525	Phosphorylation	KETEGQFYNYFPN-- CCCCCCCCCCCCC--	10.89	23616576

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
219	Y	Phosphorylation	Kinase	ABL1	P00520	Uniprot
265	Y	Phosphorylation	Kinase	ABL1	P00520	Uniprot
304	Y	Phosphorylation	Kinase	ABL1	P00520	Uniprot
508	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
508	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
525	Y	Phosphorylation	Kinase	ITK	Q03526	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
52	S	Phosphorylation	Phosphorylations at Ser-52, Tyr-76, Ser-224 and Ser-508 are regulated by mTORC1.	28424242
224	S	Phosphorylation	Phosphorylations at Ser-52, Tyr-76, Ser-224 and Ser-508 are regulated by mTORC1.	28424242
302	T	Phosphorylation	Phosphorylation at Thr-302 is required for its interaction with NFATC2.	23616576
302	T	Phosphorylation	Ubiquitination is essential for controlling protein stability, binding to the T-box-binding element of the IFN-gamma promoter, and for interaction with NFATC2 through induction of phosphorylation at Thr-302.	23616576
302	T	ubiquitylation	Ubiquitination is essential for controlling protein stability, binding to the T-box-binding element of the IFN-gamma promoter, and for interaction with NFATC2 through induction of phosphorylation at Thr-302.	23616576
313	K	ubiquitylation	Ubiquitinated at Lys-313, leading to its degradation by the proteasome.	23616576
508	S	Phosphorylation	Phosphorylations at Ser-52, Tyr-76, Ser-224 and Ser-508 are regulated by mTORC1.	21151104

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TBX21_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SMCA4_MOUSE	Smarca4	physical	21095589
BCL6_MOUSE	Bcl6	physical	22406686

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.