TBB3_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TBB3_RAT
• UniProt AC: Q4QRB4
• Protein Name: Tubulin beta-3 chain
• Gene Name: Tubb3
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 450
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance (By similarity)..
• Protein Sequence: MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEMYEDDDEESEAQGPK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
55	Phosphorylation	SVYYNEASSHKYVPR EEEECCCCCCCCCCE	27.14	22673903
56	Phosphorylation	VYYNEASSHKYVPRA EEECCCCCCCCCCEE	30.92	22673903
58	Acetylation	YNEASSHKYVPRAIL ECCCCCCCCCCEEEE	50.30	22902405
58	Ubiquitination	YNEASSHKYVPRAIL ECCCCCCCCCCEEEE	50.30	-
72	Phosphorylation	LVDLEPGTMDSVRSG EEECCCCCCHHHHCC	28.64	23984901
75	Phosphorylation	LEPGTMDSVRSGAFG CCCCCCHHHHCCCCC	14.49	27097102
106	Phosphorylation	NNWAKGHYTEGAELV CCCCCCCCCCHHHHH	18.37	-
115	Phosphorylation	EGAELVDSVLDVVRK CHHHHHHHHHHHHHH	19.66	23984901
136	Phosphorylation	CLQGFQLTHSLGGGT CCCCEEEEEECCCCC	9.91	30240740
138	Phosphorylation	QGFQLTHSLGGGTGS CCEEEEEECCCCCCC	24.39	30240740
143	Phosphorylation	THSLGGGTGSGMGTL EEECCCCCCCCHHHH	31.30	23984901
145	Phosphorylation	SLGGGTGSGMGTLLI ECCCCCCCCHHHHHH	25.99	30240740
168	Phosphorylation	DRIMNTFSVVPSPKV CCCCCCEECCCCCCC	21.84	23984901
172	Phosphorylation	NTFSVVPSPKVSDTV CCEECCCCCCCCCCE	26.24	30240740
216	Ubiquitination	DICFRTLKLATPTYG HHHHHHCCCCCCCCH	35.15	-
274	Phosphorylation	MPGFAPLTARGSQQY CCCCCCCCCCCCHHH	17.16	23984901
278	Phosphorylation	APLTARGSQQYRALT CCCCCCCCHHHEEEE	14.46	23984901
281	Phosphorylation	TARGSQQYRALTVPE CCCCCHHHEEEEHHH	7.10	23984901
290	Phosphorylation	ALTVPELTQQMFDAK EEEHHHHHHHHHCCC	18.32	-
310	Phosphorylation	CDPRHGRYLTVATVF CCCCCCCEEEHEEEE	16.00	26022182
315	Phosphorylation	GRYLTVATVFRGRMS CCEEEHEEEEECCCC	18.81	26022182
324	Acetylation	FRGRMSMKEVDEQML EECCCCHHHHHHHHH	47.48	72588455
338	Phosphorylation	LAIQSKNSSYFVEWI HHHHHCCCCCEEEEC	30.10	27097102
339	Phosphorylation	AIQSKNSSYFVEWIP HHHHCCCCCEEEECC	31.80	23984901
340	Phosphorylation	IQSKNSSYFVEWIPN HHHCCCCCEEEECCC	16.09	27097102
362	Ubiquitination	DIPPRGLKMSSTFIG CCCCCCCCCCCCCCC	39.29	-
379	Acetylation	TAIQELFKRISEQFT HHHHHHHHHHHHHHH	62.54	134801
409	Phosphorylation	GMDEMEFTEAESNMN CCCHHCCCHHHHHHH	22.45	12631274
420	Phosphorylation	SNMNDLVSEYQQYQD HHHHHHHHHHHHHHC	37.57	12631274
438	Formation of an isopeptide bond	EEEGEMYEDDDEESE HHHCCCCCCCCHHHH	54.37	-
438	5-glutamyl polyglutamate	EEEGEMYEDDDEESE HHHCCCCCCCCHHHH	54.37	-
444	Phosphorylation	YEDDDEESEAQGPK- CCCCCHHHHHCCCC-	35.86	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
172	S	Phosphorylation	Kinase	CDK1	P39951	Uniprot
444	S	Phosphorylation	Kinase	CSNK2A1	P19139	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
172	S	Phosphorylation	Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TBB3_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DPYL2_RAT	Dpysl2	physical	12134159

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.