TBA4A_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TBA4A_RAT
• UniProt AC: Q5XIF6
• Protein Name: Tubulin alpha-4A chain
• Gene Name: Tuba4a
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 448
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity)..
• Protein Sequence: MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
40	Trimethylation	DGQMPSDKTIGGGDD CCCCCCCCCCCCCCC	46.51	-
40	Acetylation	DGQMPSDKTIGGGDD CCCCCCCCCCCCCCC	46.51	-
40	Methylation	DGQMPSDKTIGGGDD CCCCCCCCCCCCCCC	46.51	-
41	Phosphorylation	GQMPSDKTIGGGDDS CCCCCCCCCCCCCCC	29.82	23984901
48	Phosphorylation	TIGGGDDSFTTFFCE CCCCCCCCCEEEEEE	29.27	23984901
50	Phosphorylation	GGGDDSFTTFFCETG CCCCCCCEEEEEECC	27.01	23984901
51	Phosphorylation	GGDDSFTTFFCETGA CCCCCCEEEEEECCC	16.83	23984901
56	Phosphorylation	FTTFFCETGAGKHVP CEEEEEECCCCCCCC	33.25	25575281
83	Nitrated tyrosine	DEIRNGPYRQLFHPE HHHHCCCCHHHCCHH	16.64	-
83	Nitration	DEIRNGPYRQLFHPE HHHHCCCCHHHCCHH	16.64	-
96	Ubiquitination	PEQLITGKEDAANNY HHHHCCCCHHHHHHC	43.71	-
96	Acetylation	PEQLITGKEDAANNY HHHHCCCCHHHHHHC	43.71	30595569
103	Phosphorylation	KEDAANNYARGHYTI CHHHHHHCCCCCCCC	9.48	27097102
108	Phosphorylation	NNYARGHYTIGKEII HHCCCCCCCCCHHHH	11.68	27097102
109	Phosphorylation	NYARGHYTIGKEIID HCCCCCCCCCHHHHH	19.99	27097102
112	Ubiquitination	RGHYTIGKEIIDPVL CCCCCCCHHHHHHHH	41.72	-
112	Acetylation	RGHYTIGKEIIDPVL CCCCCCCHHHHHHHH	41.72	22902405
140	Phosphorylation	QGFLVFHSFGGGTGS CEEEEEEECCCCCCC	17.70	30240740
158	Phosphorylation	SLLMERLSVDYGKKS HHHHHHHCCCCCCCC	21.11	30240740
163	Ubiquitination	RLSVDYGKKSKLEFS HHCCCCCCCCCEEEE	47.87	-
163	Acetylation	RLSVDYGKKSKLEFS HHCCCCCCCCCEEEE	47.87	3325245
164	Ubiquitination	LSVDYGKKSKLEFSI HCCCCCCCCCEEEEE	48.75	-
210	Phosphorylation	MVDNEAIYDICRRNL EECHHHHHHHHHCCC	13.30	-
223	Phosphorylation	NLDIERPTYTNLNRL CCCCCCCCHHCHHHH	49.55	27097102
224	Phosphorylation	LDIERPTYTNLNRLI CCCCCCCHHCHHHHH	9.05	27097102
225	Phosphorylation	DIERPTYTNLNRLIS CCCCCCHHCHHHHHH	35.39	27097102
232	Phosphorylation	TNLNRLISQIVSSIT HCHHHHHHHHHHHHH	20.60	28689409
237	Phosphorylation	LISQIVSSITASLRF HHHHHHHHHHHHCCC	17.05	23984901
271	Phosphorylation	RIHFPLATYAPVISA CCCCCCCCCCCCCCH	28.09	30240740
272	Phosphorylation	IHFPLATYAPVISAE CCCCCCCCCCCCCHH	11.87	-
277	Phosphorylation	ATYAPVISAEKAYHE CCCCCCCCHHHHHHH	30.52	23984901
282	Phosphorylation	VISAEKAYHEQLSVA CCCHHHHHHHHCCHH	19.56	23984901
287	Phosphorylation	KAYHEQLSVAEITNA HHHHHHCCHHHHHHH	20.98	23984901
292	Phosphorylation	QLSVAEITNACFEPA HCCHHHHHHHHCCCH	14.31	23984901
304	Ubiquitination	EPANQMVKCDPRHGK CCHHHCCCCCCCCCC	27.81	-
311	Ubiquitination	KCDPRHGKYMACCLL CCCCCCCCEEEEHHH	26.23	-
312	Phosphorylation	CDPRHGKYMACCLLY CCCCCCCEEEEHHHC	8.44	-
319	Phosphorylation	YMACCLLYRGDVVPK EEEEHHHCCCCCCCC	10.29	-
326	Acetylation	YRGDVVPKDVNAAIA CCCCCCCCCHHHHHH	63.80	66711741
326	Ubiquitination	YRGDVVPKDVNAAIA CCCCCCCCCHHHHHH	63.80	-
336	Ubiquitination	NAAIAAIKTKRSIQF HHHHHHHHCCCCEEE	43.18	-
336	Acetylation	NAAIAAIKTKRSIQF HHHHHHHHCCCCEEE	43.18	22902405
337	Phosphorylation	AAIAAIKTKRSIQFV HHHHHHHCCCCEEEE	26.10	23984901
340	Phosphorylation	AAIKTKRSIQFVDWC HHHHCCCCEEEEEEC	23.46	30411139
352	Ubiquitination	DWCPTGFKVGINYQP EECCCCEEEEEECCC	40.68	-
357	Phosphorylation	GFKVGINYQPPTVVP CEEEEEECCCCEECC	22.07	23984901
361	Phosphorylation	GINYQPPTVVPGGDL EEECCCCEECCCCCH	40.60	23984901
370	Ubiquitination	VPGGDLAKVQRAVCM CCCCCHHHHHHHHHH	46.31	-
370	Acetylation	VPGGDLAKVQRAVCM CCCCCHHHHHHHHHH	46.31	22632603
394	Ubiquitination	AWARLDHKFDLMYAK HHHHCCCCCCHHHEE	39.99	-
394	Acetylation	AWARLDHKFDLMYAK HHHHCCCCCCHHHEE	39.99	3325285
399	Phosphorylation	DHKFDLMYAKRAFVH CCCCCHHHEEEHHHH	19.10	-
401	Acetylation	KFDLMYAKRAFVHWY CCCHHHEEEHHHHHH	27.11	3325271
401	Ubiquitination	KFDLMYAKRAFVHWY CCCHHHEEEHHHHHH	27.11	-
408	Phosphorylation	KRAFVHWYVGEGMEE EEHHHHHHCCCCCCC	5.45	25403869
432	Phosphorylation	MAALEKDYEEVGIDS HHHHHHCHHHHCCCC	25.67	-
439	Phosphorylation	YEEVGIDSYEDEDEG HHHHCCCCCCCCCCC	28.62	22673903
440	Phosphorylation	EEVGIDSYEDEDEGE HHHCCCCCCCCCCCC	24.53	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TBA4A_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
40	K	Acetylation	Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen.	-
40	K	Methylation	Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TBA4A_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of TBA4A_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.