dbPTM

TBA4A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TBA4A_MOUSE
UniProt AC	P68368
Protein Name	Tubulin alpha-4A chain
Gene Name	Tuba4a
Organism	Mus musculus (Mouse).
Sequence Length	448
Subcellular Localization	Cytoplasm, cytoskeleton.
Protein Description	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain..
Protein Sequence	MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Ubiquitination	ECISVHVGQAGVQMG CEEEEEEHHHHHHHC	9.20	27667366
11	Ubiquitination	CISVHVGQAGVQMGN EEEEEEHHHHHHHCH	33.58	27667366
40	Methylation	DGQMPSDKTIGGGDD CCCCCCCCCCCCCCC	46.51	-
40	Acetylation	DGQMPSDKTIGGGDD CCCCCCCCCCCCCCC	46.51	-
41	Phosphorylation	GQMPSDKTIGGGDDS CCCCCCCCCCCCCCC	29.82	26745281
48	Phosphorylation	TIGGGDDSFTTFFCE CCCCCCCCCEEEEEE	29.27	25266776
50	Phosphorylation	GGGDDSFTTFFCETG CCCCCCCEEEEEECC	27.01	23984901
51	Phosphorylation	GGDDSFTTFFCETGA CCCCCCEEEEEECCC	16.83	23984901
54	S-nitrosocysteine	DSFTTFFCETGAGKH CCCEEEEEECCCCCC	3.90	-
54	S-nitrosylation	DSFTTFFCETGAGKH CCCEEEEEECCCCCC	3.90	24895380
54	Glutathionylation	DSFTTFFCETGAGKH CCCEEEEEECCCCCC	3.90	24333276
54	S-palmitoylation	DSFTTFFCETGAGKH CCCEEEEEECCCCCC	3.90	28526873
60	Ubiquitination	FCETGAGKHVPRAVF EEECCCCCCCCCEEE	40.68	22790023
81	Ubiquitination	VIDEIRNGPYRQLFH HHHHHHCCCCHHHCC	15.69	27667366
83	Nitrated tyrosine	DEIRNGPYRQLFHPE HHHHCCCCHHHCCHH	16.64	-
83	Nitration	DEIRNGPYRQLFHPE HHHHCCCCHHHCCHH	16.64	16800626
83	Nitration	DEIRNGPYRQLFHPE HHHHCCCCHHHCCHH	16.64	16800626
94	Phosphorylation	FHPEQLITGKEDAAN CCHHHHCCCCHHHHH	51.28	27600695
96	Ubiquitination	PEQLITGKEDAANNY HHHHCCCCHHHHHHC	43.71	27667366
96	Acetylation	PEQLITGKEDAANNY HHHHCCCCHHHHHHC	43.71	-
103	Phosphorylation	KEDAANNYARGHYTI CHHHHHHCCCCCCCC	9.48	22817900
108	Phosphorylation	NNYARGHYTIGKEII HHCCCCCCCCCHHHH	11.68	25195567
109	Phosphorylation	NYARGHYTIGKEIID HCCCCCCCCCHHHHH	19.99	19854140
112	Ubiquitination	RGHYTIGKEIIDPVL CCCCCCCHHHHHHHH	41.72	22790023
124	Ubiquitination	PVLDRIRKLSDQCTG HHHHHHHHHHHHCCC	50.39	27667366
134	Ubiquitination	DQCTGLQGFLVFHSF HHCCCCCEEEEEEEC	23.93	27667366
148	Ubiquitination	FGGGTGSGFTSLLME CCCCCCCCHHHHHHH	31.06	27667366
149	Ubiquitination	GGGTGSGFTSLLMER CCCCCCCHHHHHHHH	4.49	27667366
158	Phosphorylation	SLLMERLSVDYGKKS HHHHHHHCCCCCCCC	21.11	27180971
161	Phosphorylation	MERLSVDYGKKSKLE HHHHCCCCCCCCCEE	28.79	29472430
163	Ubiquitination	RLSVDYGKKSKLEFS HHCCCCCCCCCEEEE	47.87	27667366
163	Acetylation	RLSVDYGKKSKLEFS HHCCCCCCCCCEEEE	47.87	13670293
164	Ubiquitination	LSVDYGKKSKLEFSI HCCCCCCCCCEEEEE	48.75	27667366
165	Phosphorylation	SVDYGKKSKLEFSIY CCCCCCCCCEEEEEE	46.06	-
166	Ubiquitination	VDYGKKSKLEFSIYP CCCCCCCCEEEEEEE	62.00	-
168	Ubiquitination	YGKKSKLEFSIYPAP CCCCCCEEEEEEECC	40.88	27667366
173	Ubiquitination	KLEFSIYPAPQVSTA CEEEEEEECCCCCEE	35.48	27667366
183	Ubiquitination	QVSTAVVEPYNSILT CCCEEEEECCCCCCC	35.15	27667366
192	Ubiquitination	YNSILTTHTTLEHSD CCCCCCCCCCCCCCC	16.18	27667366
199	Ubiquitination	HTTLEHSDCAFMVDN CCCCCCCCEEEEECH	29.67	27667366
210	Phosphorylation	MVDNEAIYDICRRNL EECHHHHHHHHHCCC	13.30	22817900
217	Ubiquitination	YDICRRNLDIERPTY HHHHHCCCCCCCCCH	7.14	27667366
223	Phosphorylation	NLDIERPTYTNLNRL CCCCCCCCHHCHHHH	49.55	22817900
224	Phosphorylation	LDIERPTYTNLNRLI CCCCCCCHHCHHHHH	9.05	22817900
225	Phosphorylation	DIERPTYTNLNRLIS CCCCCCHHCHHHHHH	35.39	22817900
232	Phosphorylation	TNLNRLISQIVSSIT HCHHHHHHHHHHHHH	20.60	26824392
236	Phosphorylation	RLISQIVSSITASLR HHHHHHHHHHHHHCC	20.04	23984901
237	Phosphorylation	LISQIVSSITASLRF HHHHHHHHHHHHCCC	17.05	23984901
241	Ubiquitination	IVSSITASLRFDGAL HHHHHHHHCCCCCCC	16.07	27667366
248	Ubiquitination	SLRFDGALNVDLTEF HCCCCCCCCCCCCEE	8.63	27667366
271	Phosphorylation	RIHFPLATYAPVISA CCCCCCCCCCCCCCH	28.09	25159016
272	Phosphorylation	IHFPLATYAPVISAE CCCCCCCCCCCCCHH	11.87	25159016
277	Phosphorylation	ATYAPVISAEKAYHE CCCCCCCCHHHHHHH	30.52	25159016
277	O-linked_Glycosylation	ATYAPVISAEKAYHE CCCCCCCCHHHHHHH	30.52	30813421
280	Ubiquitination	APVISAEKAYHEQLS CCCCCHHHHHHHHCC	54.70	-
282	Phosphorylation	VISAEKAYHEQLSVA CCCHHHHHHHHCCHH	19.56	26745281
287	Phosphorylation	KAYHEQLSVAEITNA HHHHHHCCHHHHHHH	20.98	25521595
292	Phosphorylation	QLSVAEITNACFEPA HCCHHHHHHHHCCCH	14.31	23984901
295	S-nitrosocysteine	VAEITNACFEPANQM HHHHHHHHCCCHHHC	4.27	-
295	S-palmitoylation	VAEITNACFEPANQM HHHHHHHHCCCHHHC	4.27	28526873
295	S-nitrosylation	VAEITNACFEPANQM HHHHHHHHCCCHHHC	4.27	24895380
304	Ubiquitination	EPANQMVKCDPRHGK CCHHHCCCCCCCCCC	27.81	-
311	Ubiquitination	KCDPRHGKYMACCLL CCCCCCCCEEEEHHH	26.23	27667366
315	S-palmitoylation	RHGKYMACCLLYRGD CCCCEEEEHHHCCCC	0.67	28526873
316	S-palmitoylation	HGKYMACCLLYRGDV CCCEEEEHHHCCCCC	1.81	28526873
319	Phosphorylation	YMACCLLYRGDVVPK EEEEHHHCCCCCCCC	10.29	22817900
321	Ubiquitination	ACCLLYRGDVVPKDV EEHHHCCCCCCCCCH	20.64	27667366
326	Malonylation	YRGDVVPKDVNAAIA CCCCCCCCCHHHHHH	63.80	26320211
326	Ubiquitination	YRGDVVPKDVNAAIA CCCCCCCCCHHHHHH	63.80	27667366
326	Acetylation	YRGDVVPKDVNAAIA CCCCCCCCCHHHHHH	63.80	66701561
336	Ubiquitination	NAAIAAIKTKRSIQF HHHHHHHHCCCCEEE	43.18	22790023
337	Phosphorylation	AAIAAIKTKRSIQFV HHHHHHHCCCCEEEE	26.10	26643407
340	Phosphorylation	AAIKTKRSIQFVDWC HHHHCCCCEEEEEEC	23.46	25521595
347	S-nitrosylation	SIQFVDWCPTGFKVG CEEEEEECCCCEEEE	1.55	24895380
347	S-palmitoylation	SIQFVDWCPTGFKVG CEEEEEECCCCEEEE	1.55	28526873
347	S-nitrosocysteine	SIQFVDWCPTGFKVG CEEEEEECCCCEEEE	1.55	-
349	Phosphorylation	QFVDWCPTGFKVGIN EEEEECCCCEEEEEE	52.71	25159016
352	Ubiquitination	DWCPTGFKVGINYQP EECCCCEEEEEECCC	40.68	-
352	Acetylation	DWCPTGFKVGINYQP EECCCCEEEEEECCC	40.68	13670321
355	Ubiquitination	PTGFKVGINYQPPTV CCCEEEEEECCCCEE	4.93	27667366
357	Phosphorylation	GFKVGINYQPPTVVP CEEEEEECCCCEECC	22.07	25521595
361	Phosphorylation	GINYQPPTVVPGGDL EEECCCCEECCCCCH	40.60	25159016
370	Ubiquitination	VPGGDLAKVQRAVCM CCCCCHHHHHHHHHH	46.31	27667366
376	S-nitrosocysteine	AKVQRAVCMLSNTTA HHHHHHHHHHCCHHH	1.90	-
376	S-nitrosylation	AKVQRAVCMLSNTTA HHHHHHHHHHCCHHH	1.90	24895380
376	S-palmitoylation	AKVQRAVCMLSNTTA HHHHHHHHHHCCHHH	1.90	28526873
379	Phosphorylation	QRAVCMLSNTTAIAE HHHHHHHCCHHHHHH	12.78	22817900
379	Ubiquitination	QRAVCMLSNTTAIAE HHHHHHHCCHHHHHH	12.78	27667366
381	Phosphorylation	AVCMLSNTTAIAEAW HHHHHCCHHHHHHHH	17.77	23984901
382	Phosphorylation	VCMLSNTTAIAEAWA HHHHCCHHHHHHHHH	21.95	23984901
386	Ubiquitination	SNTTAIAEAWARLDH CCHHHHHHHHHHCCC	38.33	27667366
394	Ubiquitination	AWARLDHKFDLMYAK HHHHCCCCCCHHHEE	39.99	27667366
399	Phosphorylation	DHKFDLMYAKRAFVH CCCCCHHHEEEHHHH	19.10	26745281
401	Ubiquitination	KFDLMYAKRAFVHWY CCCHHHEEEHHHHHH	27.11	27667366
401	Acetylation	KFDLMYAKRAFVHWY CCCHHHEEEHHHHHH	27.11	13670323
408	Phosphorylation	KRAFVHWYVGEGMEE EEHHHHHHCCCCCCC	5.45	25777480
419	Phosphorylation	GMEEGEFSEAREDMA CCCCCCHHHHHHHHH	26.50	22817900
432	Phosphorylation	MAALEKDYEEVGIDS HHHHHHCHHHHCCCC	25.67	22817900
439	Phosphorylation	YEEVGIDSYEDEDEG HHHHCCCCCCCCCCC	28.62	26745281
440	Phosphorylation	EEVGIDSYEDEDEGE HHHCCCCCCCCCCCC	24.53	26745281

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of TBA4A_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
40	K	Acetylation	-
Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen.
40	K	Methylation	-
Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TBA4A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of TBA4A_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TBA4A_MOUSE

Related Literatures of Post-Translational Modification

Nitration
Reference	PubMed
"Endogenously nitrated proteins in mouse brain: links toneurodegenerative disease."; Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H.,Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J.,Squier T.C., Bigelow D.J.; Biochemistry 45:8009-8022(2006). Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-83, AND MASS SPECTROMETRY.	16800626 [Abstract]
Phosphorylation
Reference	PubMed
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-319, AND MASSSPECTROMETRY.	18034455 [Abstract]

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