dbPTM

TARA_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TARA_MOUSE
UniProt AC	Q99KW3
Protein Name	TRIO and F-actin-binding protein
Gene Name	Triobp
Organism	Mus musculus (Mouse).
Sequence Length	2014
Subcellular Localization	Nucleus. Cytoplasm, cytoskeleton. Localized to F-actin in a periodic pattern.. Isoform 1: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody. Centrosomal localization occurs upon phosphorylation by PLK1 at Thr-
Protein Description	May regulate actin cytoskeletal organization, cell spreading and cell contraction by directly binding and stabilizing filamentous F-actin. The localized formation of TARA and TRIO complexes coordinates the amount of F-actin present in stress fibers. May also serve as a linker protein to recruit proteins required for F-actin formation and turnover..
Protein Sequence	MSMEQDTRALLPTQGTAWATASAPVARLQGPQGDSHQACSQEPHSPSSAEAPYCDLPRCPPALQNPLRTTTCVGQSVHSLGLGLGQEPQRVWSPTTALPAEGPAAAPKNRHQDSEGIPYLEGLARSSCTDDNDNKDEDEDPNSNTSSSQDSNTPHDTSNSSSVDWDTTERPGVVPSRNRLTEMIPRRPQEGLRADSARKATRSPARGDTAGQRKENSGSGGQSAGQHWAKLRSESGYFSLERQRSGQTQASSGTPPSGPRGTTQASSAQRDVFQAAPAQEAPQTSSLPRNTQRDTQRSTPRTSSPSRVSQRDTPRVMSTQRKNTPLSSPLRATPETLKISAPEDGTHVTPSPCVQDSSLNRTSQRDSSRTPCIQWDNPRASSPNRTTQRDNPRTPCTQRDNPRASSPNRTTQRDNPRTPCTQRDNPRASSPNRTTQRDNPRTPCAQRDNPRAASPNRSTQRDSPRTPCAQRDNPRASSPNRTAQRDNPRTPCAQRDNPRTSCTSQNTPRTPSTQADKTTASCSKWEHLRSACTQRDNPRTFSQGCTQKDNPGPPSPRRATQGSNSRNPSPHRTNKDIPWASFPLRPTQSDSPRTSSPSRTKQNQVPWASISLRPTQGDKPQTSAPTRLAHNDPPQQYSPSLATTSSSSHNPGHSSASRTSSPLHAAPRGAPQTSLESSQPPCTVCIGHRDAPRASSPPRYFQYDPFPFFPDPRSSESESPHHEPPYMPPAVCIGHRDAPRATSPPRHTQFDPFPFLPDTSDADNESPQHDPPQFPPPVCIGYRDAPRASSPPRQFPEPSFFQDLPRASTESLVPSTDSMHEPPHIPTPVCIGHRDAPSFSSPPRQAPEPSLFFQDPPGTSMESLAPSIDSLHGCPLLPPQVCIGHRDAPRASSPPRHPPSDIGLLAPSPPPGSSGSRGSAPPGETRHNLEREEYTMLADLPPPRRLAQRGPEPQAQGSNEGRTRSPGRAEVERLFGQERRKSEAPGAFQTRDEGRSQRPSQAQSQLRRQSSPAPSRQVTKPSAKQAEPTRQSRTGPPHPKSPDKRPEGDRQLQRTSPPARTPARPPERKAQIERHLESGHTGPRQSLGGWQSQERLSGPQSPNRHPEKSWGSQKEGPSLGGWPELEGPSLEGIWRGPPQEHREQWGHSEAWEEPPSNGIQGAPPRGQGRLQELSRPHQPTPSSENSWAGPAECSCALQPEASTAVGWRAEGTSPHQRSAERPPDLDWRDLLGLLRAPEDGAWTRLPRLDWEGLLELLQARLPQKDPARHWHDPAKASGPEQGSSGTEDTLKTEPQTQPEGRAKATLANGHRPGQQSESPAQLPSPACTSTQWPTTKVTSGPETSPLAALEQIDHLESHSPPDLEFQPEEPEASEPSRGEDSRAVQKQADSADKRPAEGKAGSPLKGRLVTSWRMPGDRPALFNPYLLSLGVLRWQRPDLLNFKKGWMSILDEPGEWKKHWFVLTDSSLKYYRDSTAEEADELDGEIDLRSCTDVTEYAVQRNYGFQIHTKDAVYTLSAMTSGIRRNWIEALRKTVRPTSAPDVTKLSDCNKENTLHGYGTQKSSLKIGEQRTGSEVIGRGGPRKADGPRPSLDYVELSPLAPSSPQRMRTLSRSTPERPTKQEDLERDLAQRSEERRKWFESTDGRTPETPSGDGSRRGLGAPLTDDQQSRLSEEIEKKWQELEKLPLRENKRVPLTALLNQAHNDRRGPTSDSHEALEKEVQSLRAQLEAWRLRGEAPQNAPRLQEDSHIPPGYISQEACERSLAEMESSHQQVMEQLQRHHERELQRLQQEKEWLLAEETAATASAIEAMKKAYQEELSRELSKTRSLQQGPESLRKQHQLDMEALKQELQVLSERYSQKCLEIGALTRQAEEREHTLRRCQQEGQELLRHNQELHSHLSEEIDRLRSFIASQGTGNSCGRSNERSSCELEVLLRVKENELQYLKKEVQCLRDELQVIQKDKRFTGKYQDVYVELNHIKTRSEREIEQLKEHLRLAMAALQEKEAVRNSLAE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
45	Phosphorylation	ACSQEPHSPSSAEAP CCCCCCCCCCCCCCC	36.87	27149854
93	Phosphorylation	QEPQRVWSPTTALPA CCCCCEECCCCCCCC	14.98	28066266
95	Phosphorylation	PQRVWSPTTALPAEG CCCEECCCCCCCCCC	21.13	28066266
96	Phosphorylation	QRVWSPTTALPAEGP CCEECCCCCCCCCCC	29.58	28066266
201	Phosphorylation	ADSARKATRSPARGD HHHHHHHHCCCCCCC	34.41	29899451
203	Phosphorylation	SARKATRSPARGDTA HHHHHHCCCCCCCCC	20.68	29899451
235	Phosphorylation	WAKLRSESGYFSLER HHHHHHCCCCEEEEE	39.79	26239621
237	Phosphorylation	KLRSESGYFSLERQR HHHHCCCCEEEEEHH	10.16	29514104
239	Phosphorylation	RSESGYFSLERQRSG HHCCCCEEEEEHHCC	22.34	28066266
298	Phosphorylation	TQRDTQRSTPRTSSP CCCCCCCCCCCCCCC	32.71	24719451
302	Phosphorylation	TQRSTPRTSSPSRVS CCCCCCCCCCCCCCC	34.46	24719451
318	Phosphorylation	RDTPRVMSTQRKNTP CCCCCCCCCCCCCCC	20.66	24719451
319	Phosphorylation	DTPRVMSTQRKNTPL CCCCCCCCCCCCCCC	17.81	20469934
324	Phosphorylation	MSTQRKNTPLSSPLR CCCCCCCCCCCCCCC	28.74	23649490
327	Phosphorylation	QRKNTPLSSPLRATP CCCCCCCCCCCCCCC	30.98	28066266
328	Phosphorylation	RKNTPLSSPLRATPE CCCCCCCCCCCCCCC	35.32	22006019
381	Phosphorylation	QWDNPRASSPNRTTQ CCCCCCCCCCCCCCC	47.83	29899451
382	Phosphorylation	WDNPRASSPNRTTQR CCCCCCCCCCCCCCC	25.80	25266776
405	Phosphorylation	QRDNPRASSPNRTTQ CCCCCCCCCCCCCCC	47.83	29899451
429	Phosphorylation	QRDNPRASSPNRTTQ CCCCCCCCCCCCCCC	47.83	29899451
445 (in isoform 1)	Phosphorylation	-	14.51	-
454	Phosphorylation	RDNPRAASPNRSTQR CCCCCCCCCCCCCCC	23.02	29899451
471	Methylation	PRTPCAQRDNPRASS CCCCCHHCCCCCCCC	27.44	16289379
555	Phosphorylation	KDNPGPPSPRRATQG CCCCCCCCCCCCCCC	33.88	25266776
569	Phosphorylation	GSNSRNPSPHRTNKD CCCCCCCCCCCCCCC	36.57	-
638	Phosphorylation	NDPPQQYSPSLATTS CCCCHHCCCCCCCCC	12.04	29514104
659	Phosphorylation	GHSSASRTSSPLHAA CCCCCCCCCCCCCCC	30.94	28066266
660	Phosphorylation	HSSASRTSSPLHAAP CCCCCCCCCCCCCCC	28.93	28066266
661	Phosphorylation	SSASRTSSPLHAAPR CCCCCCCCCCCCCCC	30.76	25266776
695	Phosphorylation	HRDAPRASSPPRYFQ CCCCCCCCCCCCCCC	44.66	26239621
696	Phosphorylation	RDAPRASSPPRYFQY CCCCCCCCCCCCCCC	37.63	26239621
700	Phosphorylation	RASSPPRYFQYDPFP CCCCCCCCCCCCCCC	10.78	23984901
703	Phosphorylation	SPPRYFQYDPFPFFP CCCCCCCCCCCCCCC	18.71	23984901
714	Phosphorylation	PFFPDPRSSESESPH CCCCCCCCCCCCCCC	43.79	26060331
715	Phosphorylation	FFPDPRSSESESPHH CCCCCCCCCCCCCCC	46.55	26060331
717	Phosphorylation	PDPRSSESESPHHEP CCCCCCCCCCCCCCC	45.37	26060331
719	Phosphorylation	PRSSESESPHHEPPY CCCCCCCCCCCCCCC	38.52	26060331
742	Phosphorylation	HRDAPRATSPPRHTQ CCCCCCCCCCCCCCC	42.94	27087446
743	Phosphorylation	RDAPRATSPPRHTQF CCCCCCCCCCCCCCC	31.74	27087446
789	Phosphorylation	YRDAPRASSPPRQFP ECCCCCCCCCCCCCC	44.66	26239621
790	Phosphorylation	RDAPRASSPPRQFPE CCCCCCCCCCCCCCC	37.63	26239621
799	Phosphorylation	PRQFPEPSFFQDLPR CCCCCCCCHHHCCCC	36.71	26239621
840	Phosphorylation	HRDAPSFSSPPRQAP CCCCCCCCCCCCCCC	46.39	28066266
841	Phosphorylation	RDAPSFSSPPRQAPE CCCCCCCCCCCCCCC	36.59	23527152
892	Phosphorylation	HRDAPRASSPPRHPP CCCCCCCCCCCCCCC	44.66	25266776
893	Phosphorylation	RDAPRASSPPRHPPS CCCCCCCCCCCCCCC	37.63	25266776
900	Phosphorylation	SPPRHPPSDIGLLAP CCCCCCCCCCEEECC	46.43	25168779
908	Phosphorylation	DIGLLAPSPPPGSSG CCEEECCCCCCCCCC	44.42	25266776
913	Phosphorylation	APSPPPGSSGSRGSA CCCCCCCCCCCCCCC	36.98	29233185
914	Phosphorylation	PSPPPGSSGSRGSAP CCCCCCCCCCCCCCC	46.86	29550500
916	Phosphorylation	PPPGSSGSRGSAPPG CCCCCCCCCCCCCCC	35.10	29233185
958	Phosphorylation	PEPQAQGSNEGRTRS CCCCCCCCCCCCCCC	21.45	-
965	Phosphorylation	SNEGRTRSPGRAEVE CCCCCCCCCCHHHHH	30.97	25266776
973	Methylation	PGRAEVERLFGQERR CCHHHHHHHHCCHHH	40.26	18961603
973	Dimethylation	PGRAEVERLFGQERR CCHHHHHHHHCCHHH	40.26	-
1022	Phosphorylation	SRQVTKPSAKQAEPT CCCCCCCCHHHCCCC	50.05	21454597
1041	Phosphorylation	TGPPHPKSPDKRPEG CCCCCCCCCCCCCCC	42.66	-
1055	Phosphorylation	GDRQLQRTSPPARTP CCCCCCCCCCCCCCC	32.40	-
1056	Phosphorylation	DRQLQRTSPPARTPA CCCCCCCCCCCCCCC	30.95	25338131
1061	Phosphorylation	RTSPPARTPARPPER CCCCCCCCCCCCHHH	24.33	25266776
1097	Phosphorylation	WQSQERLSGPQSPNR CCCCHHCCCCCCCCC	55.43	28066266
1101	Phosphorylation	ERLSGPQSPNRHPEK HHCCCCCCCCCCCCC	27.55	26824392
1284	Phosphorylation	SGPEQGSSGTEDTLK CCCCCCCCCCCCCCC	57.46	24719451
1286	Phosphorylation	PEQGSSGTEDTLKTE CCCCCCCCCCCCCCC	32.41	24719451
1359	Phosphorylation	IDHLESHSPPDLEFQ HHHHHHCCCCCCCCC	47.62	25338131
1538	Phosphorylation	LRKTVRPTSAPDVTK HHHHCCCCCCCCCCC	27.27	27149854
1539	Phosphorylation	RKTVRPTSAPDVTKL HHHCCCCCCCCCCCH	40.25	29514104
1544	Phosphorylation	PTSAPDVTKLSDCNK CCCCCCCCCHHHCCC	33.38	21454597
1558	Phosphorylation	KENTLHGYGTQKSSL CCCCCCCCCCCCCCC	13.27	29514104
1560	Phosphorylation	NTLHGYGTQKSSLKI CCCCCCCCCCCCCCC	25.08	22817900
1563	Phosphorylation	HGYGTQKSSLKIGEQ CCCCCCCCCCCCCCC	30.96	25338131
1564	Phosphorylation	GYGTQKSSLKIGEQR CCCCCCCCCCCCCCC	40.49	29514104
1572	Phosphorylation	LKIGEQRTGSEVIGR CCCCCCCCCCCCCCC	44.76	29514104
1574	Phosphorylation	IGEQRTGSEVIGRGG CCCCCCCCCCCCCCC	28.25	29176673
1579	Methylation	TGSEVIGRGGPRKAD CCCCCCCCCCCCCCC	35.45	24129315
1583	Methylation	VIGRGGPRKADGPRP CCCCCCCCCCCCCCC	50.50	16288013
1591	Phosphorylation	KADGPRPSLDYVELS CCCCCCCCCCEEECC	34.84	25521595
1594	Phosphorylation	GPRPSLDYVELSPLA CCCCCCCEEECCCCC	10.91	27742792
1598	Phosphorylation	SLDYVELSPLAPSSP CCCEEECCCCCCCCH	12.36	27087446
1603	Phosphorylation	ELSPLAPSSPQRMRT ECCCCCCCCHHHHHH	49.39	25521595
1604	Phosphorylation	LSPLAPSSPQRMRTL CCCCCCCCHHHHHHH	24.80	27087446
1610	Phosphorylation	SSPQRMRTLSRSTPE CCHHHHHHHCCCCCC	21.53	28576409
1612	Phosphorylation	PQRMRTLSRSTPERP HHHHHHHCCCCCCCC	24.46	25338131
1614	Phosphorylation	RMRTLSRSTPERPTK HHHHHCCCCCCCCCC	44.96	26643407
1615	Phosphorylation	MRTLSRSTPERPTKQ HHHHCCCCCCCCCCH	28.23	26643407
1620	Phosphorylation	RSTPERPTKQEDLER CCCCCCCCCHHHHHH	52.13	25777480
1642	Phosphorylation	ERRKWFESTDGRTPE HHHHHHHCCCCCCCC	23.48	26643407
1643	Phosphorylation	RRKWFESTDGRTPET HHHHHHCCCCCCCCC	34.96	26643407
1647	Phosphorylation	FESTDGRTPETPSGD HHCCCCCCCCCCCCC	30.88	26643407
1650	Phosphorylation	TDGRTPETPSGDGSR CCCCCCCCCCCCCCC	24.77	26643407
1652	Phosphorylation	GRTPETPSGDGSRRG CCCCCCCCCCCCCCC	57.86	26643407
1656	Phosphorylation	ETPSGDGSRRGLGAP CCCCCCCCCCCCCCC	24.44	26643407
1673	Phosphorylation	DDQQSRLSEEIEKKW HHHHHHHHHHHHHHH	31.88	25338131
1836	Phosphorylation	SLQQGPESLRKQHQL HHHHCHHHHHHHHHC	36.29	25338131
1879	Phosphorylation	QAEEREHTLRRCQQE HHHHHHHHHHHHHHH	19.73	-
1924	Phosphorylation	TGNSCGRSNERSSCE CCCCCCCCCCCCCEE	28.46	23984901
1928	Phosphorylation	CGRSNERSSCELEVL CCCCCCCCCEEEEEE	32.91	23984901
1929	Phosphorylation	GRSNERSSCELEVLL CCCCCCCCEEEEEEE	20.17	23984901
1974	Phosphorylation	TGKYQDVYVELNHIK CCCCCEEEEEHHHCC	9.12	20116462
2011	Phosphorylation	EKEAVRNSLAE---- HHHHHHHHHCC----	20.56	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1879	T	Phosphorylation	Kinase	PLK1	Q07832	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Hectd3	Q3U487	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
445	T	Phosphorylation		-
[Isoform 1]: Phosphorylation at Thr-445 by PLK1 ensures mitotic progression and is essential for accurate chromosome segregation.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TARA_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of TARA_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TARA_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1604, AND MASSSPECTROMETRY.	17242355 [Abstract]

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