SYNJ1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SYNJ1_RAT
• UniProt AC: Q62910
• Protein Name: Synaptojanin-1
• Gene Name: Synj1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1574
• Subcellular Localization: Cytoplasm, perinuclear region .
• Protein Description: Phosphatase that acts on various phosphoinositides, including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate (By similarity). Has a role in clathrin-mediated endocytosis. [PubMed: 9428629 Hydrolyzes PIP2 bound to actin regulatory proteins resulting in the rearrangement of actin filaments downstream of tyrosine kinase and ASH/GRB2 (By similarity]
• Protein Sequence: MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSVLKPQVQKFLDYGFFYFDGSAVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQENTFFDDALIDELLQQFAHFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITITLKSPDWIKTLEEEMSLEKISVTLPSSTSSTLLGEDAEVSADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTAPEYSAPSLPIRPSRAPSRTPGPLSSQGAPVDTQPAAQKESSQTIEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVTRREMEAPKSPGTARKDNIGRNQPSPQAGLAGPGPSGYGAARPTIPARAGVISAPQSQARVSAGRLTPESQSKPLETSKGPAVLPEPLKPQAAFPPQPSLPTPAQKLQDPLVPIAAPMPPSIPQSNLETPPLPPPRSRSSQSLPSDSSPQLQQEQPTGQQVKINGACGVKQEPTLKSDPFEDLSLSVLAVSKAQPSAQISPVLTPDPKMLIQLPSASQSKVNSLSSVSCMLTMPPVPEQSKSQESVGSSANPFPSLPTRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVSNSPFPPLMPLSHDMSKPSSSLDGFEDNFDLQSQSTVKTSNPKGWVTFDEDEDFPTKGKSRSVYPDSLGNTAASFDDDWSKGTNVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLSSKASPTLDFTER

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Acetylation	---MAFSKGFRIYHK ---CCCCCCEEEEEC	56.46	22902405
60	Phosphorylation	YAKVLDAYGLLGVLR HHHHHHHHHHHHHEE	14.15	-
295	Acetylation	TLKDLYGKQIVVNLL HHHHHHCCCCEEEHH	24.24	22902405
305	Acetylation	VVNLLGSKEGEHMLS EEEHHCCCHHHHHHH	68.86	22902405
352	Acetylation	EKLHSVLKPQVQKFL HHHHHHHHHHHHHHH	31.11	22902405
414	Acetylation	EALGLAEKPQLVTRF HHCCCCCCHHHHHHH	32.24	22902405
499	Phosphorylation	KARALLTTGSLRVSE HHHHHHHHCCCCCCH	25.41	27097102
501	Phosphorylation	RALLTTGSLRVSEQT HHHHHHCCCCCCHHH	15.77	27097102
515	Ubiquitination	TLQSASSKVLKNMCE HHHHHCHHHHHHHHH	49.44	-
577	Ubiquitination	GIQEFQDKRSKPTDI CHHHHHHCCCCCCCE	48.03	-
659	Ubiquitination	DVAVDTVKTGMGGAT EEEECCCCCCCCCCC	41.26	-
744 (in isoform 6)	Phosphorylation	-	55.39	26437020
747 (in isoform 6)	Phosphorylation	-	44.66	26437020
783	Phosphorylation	GKVTFAPTYKYDLFS CCEEECCEECEECCC	29.26	-
784	Phosphorylation	KVTFAPTYKYDLFSE CEEECCEECEECCCC	13.39	-
786	Phosphorylation	TFAPTYKYDLFSEDY EECCEECEECCCCCC	13.89	-
820	Phosphorylation	RKWPFDRSAEDLDLL CCCCCCCCHHHHHHH	37.54	22673903
830	Phosphorylation	DLDLLNASFQDESKI HHHHHCCCCCCCCCE	23.51	23984901
835	Phosphorylation	NASFQDESKILYTWT CCCCCCCCCEEEEEC	33.03	23984901
982	Phosphorylation	KTLEEEMSLEKISVT HHHHHHHCCCEEEEE	36.33	25403869
1048	Phosphorylation	SPSSSPRTSPCQSPT CCCCCCCCCCCCCCC	40.17	27097102
1049	Phosphorylation	PSSSPRTSPCQSPTA CCCCCCCCCCCCCCC	25.59	30411139
1053	Phosphorylation	PRTSPCQSPTAPEYS CCCCCCCCCCCCCCC	30.82	23712012
1055	Phosphorylation	TSPCQSPTAPEYSAP CCCCCCCCCCCCCCC	61.15	27097102
1059	Phosphorylation	QSPTAPEYSAPSLPI CCCCCCCCCCCCCCC	14.74	27097102
1060	Phosphorylation	SPTAPEYSAPSLPIR CCCCCCCCCCCCCCC	31.85	28551015
1063	Phosphorylation	APEYSAPSLPIRPSR CCCCCCCCCCCCCCC	46.63	28551015
1073	Phosphorylation	IRPSRAPSRTPGPLS CCCCCCCCCCCCCCC	48.04	28432305
1075	Phosphorylation	PSRAPSRTPGPLSSQ CCCCCCCCCCCCCCC	36.61	28432305
1080	Phosphorylation	SRTPGPLSSQGAPVD CCCCCCCCCCCCCCC	24.61	28432305
1081	Phosphorylation	RTPGPLSSQGAPVDT CCCCCCCCCCCCCCC	40.07	28432305
1131	Phosphorylation	PPPSGARSPAPARKE CCCCCCCCCCCCCHH	25.53	25403869
1144 (in isoform 5)	Phosphorylation	-	15.49	26437020
1144 (in isoform 4)	Phosphorylation	-	15.49	26437020
1147 (in isoform 4)	Phosphorylation	-	32.97	26437020
1147	Phosphorylation	GGVGAPPSPGVTRRE CCCCCCCCCCCCHHH	32.97	28551015
1147 (in isoform 5)	Phosphorylation	-	32.97	26437020
1151	Phosphorylation	APPSPGVTRREMEAP CCCCCCCCHHHCCCC	29.89	28551015
1160	Phosphorylation	REMEAPKSPGTARKD HHCCCCCCCCCCCCC	27.74	28551015
1175	Phosphorylation	NIGRNQPSPQAGLAG CCCCCCCCCCCCCCC	21.63	29779826
1186	Phosphorylation	GLAGPGPSGYGAARP CCCCCCCCCCCCCCC	51.03	23984901
1188	Phosphorylation	AGPGPSGYGAARPTI CCCCCCCCCCCCCCC	14.13	23984901
1194	Phosphorylation	GYGAARPTIPARAGV CCCCCCCCCCCCCCC	33.76	23984901
1198	Methylation	ARPTIPARAGVISAP CCCCCCCCCCCEECC	26.90	-
1212	Phosphorylation	PQSQARVSAGRLTPE CHHHHHHCCCCCCHH	21.32	28432305
1217	Phosphorylation	RVSAGRLTPESQSKP HHCCCCCCHHHHCCC	24.53	29779826
1220	Phosphorylation	AGRLTPESQSKPLET CCCCCHHHHCCCCCC	40.74	30240740
1222	Phosphorylation	RLTPESQSKPLETSK CCCHHHHCCCCCCCC	45.58	25403869
1227	Phosphorylation	SQSKPLETSKGPAVL HHCCCCCCCCCCCCC	43.34	28432305
1228	Phosphorylation	QSKPLETSKGPAVLP HCCCCCCCCCCCCCC	26.66	28432305
1287	Phosphorylation	PPLPPPRSRSSQSLP CCCCCCCCCCCCCCC	42.14	27097102
1289	Phosphorylation	LPPPRSRSSQSLPSD CCCCCCCCCCCCCCC	33.69	27097102
1290	Phosphorylation	PPPRSRSSQSLPSDS CCCCCCCCCCCCCCC	23.76	27097102
1292	Phosphorylation	PRSRSSQSLPSDSSP CCCCCCCCCCCCCCH	44.17	27097102
1295	Phosphorylation	RSSQSLPSDSSPQLQ CCCCCCCCCCCHHHH	56.25	27097102
1297	Phosphorylation	SQSLPSDSSPQLQQE CCCCCCCCCHHHHCC	48.53	27097102
1298	Phosphorylation	QSLPSDSSPQLQQEQ CCCCCCCCHHHHCCC	22.96	27097102
1346	Phosphorylation	AVSKAQPSAQISPVL EEECCCCCCCCCCCC	22.26	23984901
1350	Phosphorylation	AQPSAQISPVLTPDP CCCCCCCCCCCCCCH	9.25	28689409
1354	Phosphorylation	AQISPVLTPDPKMLI CCCCCCCCCCHHHEE	26.36	28689409
1427	Phosphorylation	GNPFRVQSQESEATS CCCCEEECCCHHCCC	32.81	28432305
1489	Phosphorylation	SNPKGWVTFDEDEDF CCCCCEEEECCCCCC	20.89	28432305
1516	Phosphorylation	SLGNTAASFDDDWSK CCCCCCCCCCCCCCC	27.09	28432305
1566	Phosphorylation	TTLSSKASPTLDFTE CCCCCCCCCCCCCCC	23.35	29779826
1568	Phosphorylation	LSSKASPTLDFTER- CCCCCCCCCCCCCC-	36.14	22673903
1572	Phosphorylation	ASPTLDFTER----- CCCCCCCCCC-----	30.43	28689409

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1018	Y	Phosphorylation	Kinase	EPHB2	A0A0G2JY48	GPS
1059	Y	Phosphorylation	Kinase	EPHB2	A0A0G2JY48	GPS
1144	S	Phosphorylation	Kinase	CDK5	Q03114	PSP
1160	S	Phosphorylation	Kinase	CDK5	Q03114	PSP
1172	Y	Phosphorylation	Kinase	EPHB2	A0A0G2JY48	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SYNJ1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SYNJ1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EPS15_HUMAN	EPS15	physical	9428629

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.