dbPTM

SYN1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SYN1_MOUSE
UniProt AC	O88935
Protein Name	Synapsin-1
Gene Name	Syn1
Organism	Mus musculus (Mouse).
Sequence Length	706
Subcellular Localization	Cell junction, synapse . Golgi apparatus .
Protein Description	Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level..
Protein Sequence	MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPSAASPGATPGSATASAERASTAAPVASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAAARVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVKVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMTQALPRQPQRDASPGRGSHSQSSSPGALTLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTAAPQQSASQATPVTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGAPQATRQASISGPAPTKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPGPRTGPPTTQQPRPSGPGPAGRPAKPQLAQKPSQDVPPPITAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASLFSD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	NYLRRRLSDSNFMAN CHHHHHCCCCCHHHC	36.86	21930439
11	Phosphorylation	LRRRLSDSNFMANLP HHHHCCCCCHHHCCC	28.70	21930439
21	Phosphorylation	MANLPNGYMTDLQRP HHCCCCCCCCCCCCC	11.95	29899451
23	Phosphorylation	NLPNGYMTDLQRPQP CCCCCCCCCCCCCCC	25.69	20415495
36	Phosphorylation	QPPPPPPSAASPGAT CCCCCCCCCCCCCCC	42.09	22817900
39	Phosphorylation	PPPPSAASPGATPGS CCCCCCCCCCCCCCC	24.47	22817900
43	Phosphorylation	SAASPGATPGSATAS CCCCCCCCCCCHHHC	33.81	20415495
46	Phosphorylation	SPGATPGSATASAER CCCCCCCCHHHCHHH	24.53	20415495
48	Phosphorylation	GATPGSATASAERAS CCCCCCHHHCHHHHH	23.89	20415495
50	Phosphorylation	TPGSATASAERASTA CCCCHHHCHHHHHCC	26.72	20415495
55	O-linked_Glycosylation	TASAERASTAAPVAS HHCHHHHHCCCCCCC	25.46	-
55	Phosphorylation	TASAERASTAAPVAS HHCHHHHHCCCCCCC	25.46	24925903
56	O-linked_Glycosylation	ASAERASTAAPVASP HCHHHHHCCCCCCCC	26.34	-
56	Phosphorylation	ASAERASTAAPVASP HCHHHHHCCCCCCCC	26.34	25521595
62	Phosphorylation	STAAPVASPAAPSPG HCCCCCCCCCCCCCC	18.46	24925903
67	Phosphorylation	VASPAAPSPGSSGGG CCCCCCCCCCCCCCC	36.85	25521595
70	Phosphorylation	PAAPSPGSSGGGGFF CCCCCCCCCCCCCHH	29.24	25521595
71	Phosphorylation	AAPSPGSSGGGGFFS CCCCCCCCCCCCHHH	47.58	25521595
78	Phosphorylation	SGGGGFFSSLSNAVK CCCCCHHHHHHHHHH	28.27	24925903
79	Phosphorylation	GGGGFFSSLSNAVKQ CCCCHHHHHHHHHHH	30.57	24925903
81	Phosphorylation	GGFFSSLSNAVKQTT CCHHHHHHHHHHHHH	25.37	24925903
87	O-linked_Glycosylation	LSNAVKQTTAAAAAT HHHHHHHHHHHHHHH	16.82	16452088
88	Phosphorylation	SNAVKQTTAAAAATF HHHHHHHHHHHHHHH	16.29	29899451
94	Phosphorylation	TTAAAAATFSEQVGG HHHHHHHHHHHHCCC	23.98	29899451
96	O-linked_Glycosylation	AAAAATFSEQVGGGS HHHHHHHHHHCCCCC	23.82	-
96	Phosphorylation	AAAAATFSEQVGGGS HHHHHHHHHHCCCCC	23.82	25521595
103	O-linked_Glycosylation	SEQVGGGSGGAGRGG HHHCCCCCCCCCCCC	37.01	-
103	Phosphorylation	SEQVGGGSGGAGRGG HHHCCCCCCCCCCCC	37.01	22817900
128	Ubiquitination	EPHTDWAKYFKGKKI CCCCHHHHHHCCCEE	47.14	22790023
129	Phosphorylation	PHTDWAKYFKGKKIH CCCHHHHHHCCCEEE	11.63	-
173	Ubiquitination	EVLRNGVKVVRSLKP HHHHCCEEEHHHCCC	36.09	27667366
177	Phosphorylation	NGVKVVRSLKPDFVL CCEEEHHHCCCCEEE	29.18	29899451
179	Ubiquitination	VKVVRSLKPDFVLIR EEEHHHCCCCEEEEE	43.73	22790023
238	Ubiquitination	QMVRLHKKLGTEEFP HHHHHHHHHCCCCCC	41.21	22790023
241	Phosphorylation	RLHKKLGTEEFPLID HHHHHHCCCCCCCCC	42.66	29899451
256	Ubiquitination	QTFYPNHKEMLSSTT CCCCCCHHHHHHCCC	52.00	22790023
261	O-linked_Glycosylation	NHKEMLSSTTYPVVV CHHHHHHCCCCEEEE	22.32	-
281	Ubiquitination	HSGMGKVKVDNQHDF CCCCCEEEECCCCCH	47.56	22790023
301	Phosphorylation	VVALTKTYATAEPFI HHHHCCCCCCCCCCC	11.96	20530578
303	Phosphorylation	ALTKTYATAEPFIDA HHCCCCCCCCCCCCC	22.57	-
311	Ubiquitination	AEPFIDAKYDVRVQK CCCCCCCCCCEEEEE	37.93	22790023
312	Phosphorylation	EPFIDAKYDVRVQKI CCCCCCCCCEEEEEC	22.76	22817900
318	Ubiquitination	KYDVRVQKIGQNYKA CCCEEEEECCCCEEE	46.04	22790023
332	Phosphorylation	AYMRTSVSGNWKTNT EEEEECCCCCCCCCC	26.61	25521595
336	Ubiquitination	TSVSGNWKTNTGSAM ECCCCCCCCCCCHHH	35.58	22790023
337	Phosphorylation	SVSGNWKTNTGSAML CCCCCCCCCCCHHHH	29.63	25521595
339	Phosphorylation	SGNWKTNTGSAMLEQ CCCCCCCCCHHHHHH	37.43	25521595
341	Phosphorylation	NWKTNTGSAMLEQIA CCCCCCCHHHHHHHH	14.41	25521595
350	Phosphorylation	MLEQIAMSDRYKLWV HHHHHHCCCCCEEHH	15.20	24925903
390	Phosphorylation	HIIEVVGSSMPLIGD CEEEEECCCCCCCCC	16.32	25521595
391	Phosphorylation	IIEVVGSSMPLIGDH EEEEECCCCCCCCCC	20.63	22817900
427	Phosphorylation	RQPQRDASPGRGSHS CCCCCCCCCCCCCCC	31.98	25521595
430	Methylation	QRDASPGRGSHSQSS CCCCCCCCCCCCCCC	46.99	24129315
432	O-linked_Glycosylation	DASPGRGSHSQSSSP CCCCCCCCCCCCCCC	20.71	-
432	Phosphorylation	DASPGRGSHSQSSSP CCCCCCCCCCCCCCC	20.71	24925903
434	Phosphorylation	SPGRGSHSQSSSPGA CCCCCCCCCCCCCCC	33.19	25521595
436	O-linked_Glycosylation	GRGSHSQSSSPGALT CCCCCCCCCCCCCEE	35.48	16452088
436	Phosphorylation	GRGSHSQSSSPGALT CCCCCCCCCCCCCEE	35.48	24925903
437	Phosphorylation	RGSHSQSSSPGALTL CCCCCCCCCCCCEEE	32.68	25521595
438	Phosphorylation	GSHSQSSSPGALTLG CCCCCCCCCCCEEEC	32.38	25521595
443	Phosphorylation	SSSPGALTLGRQTSQ CCCCCCEEECCCCCC	27.00	24925903
448	Phosphorylation	ALTLGRQTSQQPAGP CEEECCCCCCCCCCC	27.43	22817900
449	Phosphorylation	LTLGRQTSQQPAGPP EEECCCCCCCCCCCC	20.62	22817900
476	Methylation	QPGPGPQRQGPPLQQ CCCCCCCCCCCCHHC	46.70	24129315
484	Methylation	QGPPLQQRPPPQGQQ CCCCHHCCCCCCCCC	32.50	58859785
502	Phosphorylation	GLGPPAGSPLPQRLP CCCCCCCCCCCCCCC	25.93	29899451
510	Phosphorylation	PLPQRLPSPTAAPQQ CCCCCCCCCCCCCCC	39.20	25521595
512	Phosphorylation	PQRLPSPTAAPQQSA CCCCCCCCCCCCCCC	39.53	25521595
518	O-linked_Glycosylation	PTAAPQQSASQATPV CCCCCCCCCHHCCCC	25.33	16452088
518	Phosphorylation	PTAAPQQSASQATPV CCCCCCCCCHHCCCC	25.33	25521595
520	Phosphorylation	AAPQQSASQATPVTQ CCCCCCCHHCCCCCC	26.12	25521595
523	Phosphorylation	QQSASQATPVTQGQG CCCCHHCCCCCCCCC	15.62	25777480
526	O-linked_Glycosylation	ASQATPVTQGQGRQS CHHCCCCCCCCCCCC	28.34	16452088
526	Phosphorylation	ASQATPVTQGQGRQS CHHCCCCCCCCCCCC	28.34	25777480
533	Phosphorylation	TQGQGRQSRPVAGGP CCCCCCCCCCCCCCC	36.87	26804993
534	Methylation	QGQGRQSRPVAGGPG CCCCCCCCCCCCCCC	22.75	24129315
547	Methylation	PGAPPAARPPASPSP CCCCCCCCCCCCCCH	39.58	24129315
551	Phosphorylation	PAARPPASPSPQRQA CCCCCCCCCCHHHCC	31.52	25521595
553	Phosphorylation	ARPPASPSPQRQAGA CCCCCCCCHHHCCCC	31.04	25521595
556	Methylation	PASPSPQRQAGAPQA CCCCCHHHCCCCCCC	31.53	24129315
564	O-linked_Glycosylation	QAGAPQATRQASISG CCCCCCCCCCCCCCC	20.33	-
564	Phosphorylation	QAGAPQATRQASISG CCCCCCCCCCCCCCC	20.33	29899451
568	O-linked_Glycosylation	PQATRQASISGPAPT CCCCCCCCCCCCCCC	14.67	16452088
568	Phosphorylation	PQATRQASISGPAPT CCCCCCCCCCCCCCC	14.67	25521595
570	Phosphorylation	ATRQASISGPAPTKA CCCCCCCCCCCCCCC	35.91	22324799
575	Phosphorylation	SISGPAPTKASGAPP CCCCCCCCCCCCCCC	41.15	24925903
576	Methylation	ISGPAPTKASGAPPG CCCCCCCCCCCCCCC	39.22	-
576	Ubiquitination	ISGPAPTKASGAPPG CCCCCCCCCCCCCCC	39.22	22790023
577 (in isoform 3)	Phosphorylation	-	18.71	29514104
578	O-linked_Glycosylation	GPAPTKASGAPPGGQ CCCCCCCCCCCCCCC	36.72	-
587	Methylation	APPGGQQRQGPPQKP CCCCCCCCCCCCCCC	35.22	58859813
593	Ubiquitination	QRQGPPQKPPGPAGP CCCCCCCCCCCCCCC	59.98	22790023
601	Phosphorylation	PPGPAGPTRQASQAG CCCCCCCCCCHHHCC	34.20	-
605	O-linked_Glycosylation	AGPTRQASQAGPGPR CCCCCCHHHCCCCCC	16.54	20249
605	Phosphorylation	AGPTRQASQAGPGPR CCCCCCHHHCCCCCC	16.54	25521595
612	Methylation	SQAGPGPRTGPPTTQ HHCCCCCCCCCCCCC	59.22	58859799
613	Phosphorylation	QAGPGPRTGPPTTQQ HCCCCCCCCCCCCCC	58.11	24719451
618	Phosphorylation	PRTGPPTTQQPRPSG CCCCCCCCCCCCCCC	30.86	-
622	Methylation	PPTTQQPRPSGPGPA CCCCCCCCCCCCCCC	33.41	24129315
642	Phosphorylation	PQLAQKPSQDVPPPI CCCCCCCCCCCCCCC	46.15	26824392
642 (in isoform 1)	Phosphorylation	-	46.15	22324799
650	O-linked_Glycosylation	QDVPPPITAAAGGPP CCCCCCCCCCCCCCC	18.93	16452088
650 (in isoform 1)	Phosphorylation	-	18.93	20415495
664	Phosphorylation	PHPQLNKSQSLTNAF CCCCCCHHHCCHHCC	24.75	25521595
664 (in isoform 1)	Phosphorylation	-	24.75	25521595
666	Phosphorylation	PQLNKSQSLTNAFNL CCCCHHHCCHHCCCC	44.23	25521595
666 (in isoform 1)	Phosphorylation	-	44.23	25521595
668	Phosphorylation	LNKSQSLTNAFNLPE CCHHHCCHHCCCCCC	29.73	25521595
680	Asymmetric dimethylarginine	LPEPAPPRPSLSQDE CCCCCCCCCCCCHHH	32.17	-
680	Methylation	LPEPAPPRPSLSQDE CCCCCCCCCCCCHHH	32.17	24129315
682	Phosphorylation	EPAPPRPSLSQDEVK CCCCCCCCCCHHHHH	41.76	25521595
684	Phosphorylation	APPRPSLSQDEVKAE CCCCCCCCHHHHHHH	39.67	25521595
692	Phosphorylation	QDEVKAETIRSLRKS HHHHHHHHHHHHHHH	26.92	22324799
695	Phosphorylation	VKAETIRSLRKSFAS HHHHHHHHHHHHHHH	28.77	24719451
699	Phosphorylation	TIRSLRKSFASLFSD HHHHHHHHHHHHHCC	21.44	25521595
702	Phosphorylation	SLRKSFASLFSD--- HHHHHHHHHHCC---	28.12	25521595
705	Phosphorylation	KSFASLFSD------ HHHHHHHCC------	47.94	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
551	S	Phosphorylation	Kinase	PKCG	P05129	PSP
551	S	Phosphorylation	Kinase	PDPK1	Q9Z2A0	Uniprot
553	S	Phosphorylation	Kinase	PKCG	P05129	PSP
568	S	Phosphorylation	Kinase	CAMK2	-	Uniprot
605	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
605	S	Phosphorylation	Kinase	CAMK2A	P11798	PSP
605	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
605	S	Phosphorylation	Kinase	CAMK2	-	Uniprot
656	S	Phosphorylation	Kinase	ATM	Q62388	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
9	S	Phosphorylation		21183079
Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SYN1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
PRIO_MOUSE	Prnp	physical	11571277

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SYN1_MOUSE

Related Literatures of Post-Translational Modification

O-linked Glycosylation
Reference	PubMed
"O-linked N-acetylglucosamine proteomics of postsynaptic densitypreparations using lectin weak affinity chromatography and massspectrometry."; Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G.,Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F.,Maltby D.A., Schoepfer R., Burlingame A.L.; Mol. Cell. Proteomics 5:923-934(2006). Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-87 AND THR-526, AND MASSSPECTROMETRY.	16452088 [Abstract]
Phosphorylation
Reference	PubMed
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-67; THR-337;SER-427; THR-512; SER-520; SER-553; SER-666 AND SER-705, AND MASSSPECTROMETRY.	17114649 [Abstract]
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427 AND SER-568, ANDMASS SPECTROMETRY.	16452087 [Abstract]
"Phosphoproteomic analysis of the developing mouse brain."; Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.; Mol. Cell. Proteomics 3:1093-1101(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-510, AND MASSSPECTROMETRY.	15345747 [Abstract]
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-312, AND MASSSPECTROMETRY.	18034455 [Abstract]

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