dbPTM

SYEP_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SYEP_MOUSE
UniProt AC	Q8CGC7
Protein Name	Bifunctional glutamate/proline--tRNA ligase
Gene Name	Eprs
Organism	Mus musculus (Mouse).
Sequence Length	1512
Subcellular Localization	Cytoplasm, cytosol . Membrane Peripheral membrane protein . Translocates from cytosol to membranes upon phosphorylation at Ser-999.
Protein Description	Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA (By similarity). The phosphorylation of EPRS, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS function from protein synthesis to translation inhibition. [PubMed: 23071094 Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan]
Protein Sequence	MAALCLTVNAGNPPLEALLAVEHVKGDVSISVEEGKENLLRVSETVAFTDVNSILRYLARIATTSGLYGTNLMEHTEIDHWLEFSATKLSSCDRLTSAINELNHCLSLRTYLVGNSLTLADLCVWATLKGSAAWQEHLKQNKTLVHVKRWFGFLEAQQAFRSVGTKWDVSGNRATVAPDKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRTESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVVPVNVLDAQEEMKEVARHPKNPDVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNINITKIHKNADGKITSLDAKLNLENKDYKKTTKITWLAESTHALSIPAVCVTYEHLITKPVLGKDEDFKQYINKDSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCREAPCILIYIPDGHTKEMPTSGSKEKTKVEISKKETSSAPKERPAPAVSSTCATAEDSSVLYSRVAVQGDVVRELKAKKAPKEDIDAAVKQLLTLKAEYKEKTGQEYKPGNPSAAAVQTVSTKSSSNTVESTSLYNKVAAQGEVVRKLKAEKAPKAKVTEAVECLLSLKAEYKEKTGKDYVPGQPPASQNSHSNPVSNAQPAGAEKPEAKVLFDRVACQGEVVRKLKAEKASKDQVDSAVQELLQLKAQYKSLTGIEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQGKDSSKSQGSGLSSGGAGEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILRPWSYSIWESIKDFFDAEIKKLGVENCYFPIFVSQAALEKEKNHIEDFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPVRLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEAFISASGRAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVHGDNMGLVLPPRVASVQVVVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRVRVDLRDNYSPGWKFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAKLEKVLEDIQLNLFTRASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDVEPGAPSMGAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
31	Phosphorylation	VKGDVSISVEEGKEN CCCCEEEEECCCHHH	18.90	26370283
36	Ubiquitination	SISVEEGKENLLRVS EEEECCCHHHEEEEE	46.86	22790023
105	S-nitrosocysteine	AINELNHCLSLRTYL HHHHHHHHHHCCHHH	2.41	-
105	S-nitrosylation	AINELNHCLSLRTYL HHHHHHHHHHCCHHH	2.41	20925432
142	Acetylation	QEHLKQNKTLVHVKR HHHHHCCCCCHHHHH	40.52	23806337
166	Ubiquitination	AFRSVGTKWDVSGNR HHHHHCCEEECCCCC	35.15	22790023
178	Ubiquitination	GNRATVAPDKKQDVG CCCEEECCCCCCCCC	50.01	27667366
197	Ubiquitination	LPGAEMGKVTVRFPP CCCCCEEEEEEECCC	33.16	22790023
207	Phosphorylation	VRFPPEASGYLHIGH EECCCCCCCEEEEEE	26.72	22871156
209	Phosphorylation	FPPEASGYLHIGHAK CCCCCCCEEEEEEHH	7.81	22871156
224	Phosphorylation	AALLNQHYQVNFKGK HHHHCCCEEEECCCE	12.37	22871156
229	Malonylation	QHYQVNFKGKLIMRF CCEEEECCCEEEEEE	50.35	26320211
229	Ubiquitination	QHYQVNFKGKLIMRF CCEEEECCCEEEEEE	50.35	-
282	Acetylation	TIMKYAEKLIQEGKA HHHHHHHHHHHHCCC	42.59	22826441
282	Malonylation	TIMKYAEKLIQEGKA HHHHHHHHHHHHCCC	42.59	26320211
300	N6-malonyllysine	DTPAEQMKAEREQRT CCHHHHHHHHHHHHH	46.95	-
300	Acetylation	DTPAEQMKAEREQRT CCHHHHHHHHHHHHH	46.95	23806337
300	Malonylation	DTPAEQMKAEREQRT CCHHHHHHHHHHHHH	46.95	26320211
315	Phosphorylation	ESKHRKNSVEKNLQM HHHHHHHHHHHHHHH	34.39	24759943
336	Glutathionylation	GSQFGQSCCLRAKID CCCCCCCHHHEEEEE	1.62	24333276
337	Glutathionylation	SQFGQSCCLRAKIDM CCCCCCHHHEEEEEC	3.31	24333276
337	S-nitrosylation	SQFGQSCCLRAKIDM CCCCCCHHHEEEEEC	3.31	24926564
350	Glutathionylation	DMSSNNGCMRDPTLY ECCCCCCCCCCCEEE	1.88	24333276
350	S-palmitoylation	DMSSNNGCMRDPTLY ECCCCCCCCCCCEEE	1.88	28526873
355	Phosphorylation	NGCMRDPTLYRCKIQ CCCCCCCEEEEEEEE	40.48	29514104
357	Phosphorylation	CMRDPTLYRCKIQPH CCCCCEEEEEEEECC	19.22	-
360	Ubiquitination	DPTLYRCKIQPHPRT CCEEEEEEEECCCCC	35.06	22790023
377	Phosphorylation	KYNVYPTYDFACPIV CCEECCCCCCCCCCH	12.45	22817900
381	Glutathionylation	YPTYDFACPIVDSIE CCCCCCCCCCHHHHH	2.09	24333276
399	Phosphorylation	HALRTTEYHDRDEQF EEHHCCCCCCCHHHH	13.61	-
407	Phosphorylation	HDRDEQFYWIIEALG CCCHHHHHHHHHHHC	8.73	-
417	Acetylation	IEALGIRKPYIWEYS HHHHCCCCCCHHEEE	40.01	23806337
419	Phosphorylation	ALGIRKPYIWEYSRL HHCCCCCCHHEEEEC	23.00	28576409
419	Ubiquitination	ALGIRKPYIWEYSRL HHCCCCCCHHEEEEC	23.00	27667366
431	Phosphorylation	SRLNLNNTVLSKRKL EECCCCCCCCCCCCE	23.04	20415495
434	Phosphorylation	NLNNTVLSKRKLTWF CCCCCCCCCCCEEEE	26.52	26824392
435	Malonylation	LNNTVLSKRKLTWFV CCCCCCCCCCEEEEE	49.76	26320211
435	Ubiquitination	LNNTVLSKRKLTWFV CCCCCCCCCCEEEEE	49.76	27667366
467	Phosphorylation	GVLRRGMTVEGLKQF HHHHCCCCHHHHHHH	20.45	29514104
497	Acetylation	DKIWAFNKKVIDPVA HHHHEECCCCCCCCC	41.80	23236377
497	Malonylation	DKIWAFNKKVIDPVA HHHHEECCCCCCCCC	41.80	26320211
498	Acetylation	KIWAFNKKVIDPVAP HHHEECCCCCCCCCH	45.59	-
498	Malonylation	KIWAFNKKVIDPVAP HHHEECCCCCCCCCH	45.59	26320211
528	Acetylation	LDAQEEMKEVARHPK CCHHHHHHHHHHCCC	52.93	23236377
535	Acetylation	KEVARHPKNPDVGLK HHHHHCCCCCCCCCC	75.00	23954790
535	Malonylation	KEVARHPKNPDVGLK HHHHHCCCCCCCCCC	75.00	26320211
535	Ubiquitination	KEVARHPKNPDVGLK HHHHHCCCCCCCCCC	75.00	-
542	Acetylation	KNPDVGLKPVWYSPK CCCCCCCCCCEECCE	31.43	23806337
547	Phosphorylation	GLKPVWYSPKVFIEG CCCCCEECCEEEEEC	11.52	26370283
577	Ubiquitination	NWGNINITKIHKNAD ECCCEEEEEEEECCC	21.32	27667366
589	Phosphorylation	NADGKITSLDAKLNL CCCCCEEEEEEECCC	27.94	-
593	Acetylation	KITSLDAKLNLENKD CEEEEEEECCCCCCC	37.26	23954790
599	Malonylation	AKLNLENKDYKKTTK EECCCCCCCCCHHEE	53.73	26320211
602	Malonylation	NLENKDYKKTTKITW CCCCCCCCHHEEEEE	54.50	26320211
637	Acetylation	ITKPVLGKDEDFKQY HCCCCCCCCHHHHHH	54.50	23954790
642	Malonylation	LGKDEDFKQYINKDS CCCCHHHHHHCCCCC	55.29	26320211
660	S-nitrosylation	ELMLGDPCLKDLKKG HHHCCCCCHHHCCCC	9.80	24926564
680	Glutathionylation	QRRGFFICDQPYEPV EECCEEECCCCCCCC	3.16	24333276
684	Phosphorylation	FFICDQPYEPVSPYS EEECCCCCCCCCCCC	27.51	25367039
688	Phosphorylation	DQPYEPVSPYSCREA CCCCCCCCCCCCCCC	28.92	25367039
690	Phosphorylation	PYEPVSPYSCREAPC CCCCCCCCCCCCCCE	16.94	28725479
691	Phosphorylation	YEPVSPYSCREAPCI CCCCCCCCCCCCCEE	15.30	25367039
692	Glutathionylation	EPVSPYSCREAPCIL CCCCCCCCCCCCEEE	3.63	24333276
694	Ubiquitination	VSPYSCREAPCILIY CCCCCCCCCCEEEEE	62.38	27667366
697	Glutathionylation	YSCREAPCILIYIPD CCCCCCCEEEEEECC	5.22	24333276
701	Ubiquitination	EAPCILIYIPDGHTK CCCEEEEEECCCCCC	11.71	27667366
733	Malonylation	KETSSAPKERPAPAV HHCCCCCCCCCCCCC	67.10	26320211
744	S-nitrosocysteine	APAVSSTCATAEDSS CCCCCCCCCCCCCCC	3.24	-
744	Glutathionylation	APAVSSTCATAEDSS CCCCCCCCCCCCCCC	3.24	24333276
744	S-nitrosylation	APAVSSTCATAEDSS CCCCCCCCCCCCCCC	3.24	21278135
754	Phosphorylation	AEDSSVLYSRVAVQG CCCCCCEEEEEEEEC	7.71	28725479
755	Phosphorylation	EDSSVLYSRVAVQGD CCCCCEEEEEEEECH	19.00	25367039
774	Malonylation	LKAKKAPKEDIDAAV HHHCCCCHHHHHHHH	73.25	26320211
782	Ubiquitination	EDIDAAVKQLLTLKA HHHHHHHHHHHHHHH	30.42	22790023
788	Acetylation	VKQLLTLKAEYKEKT HHHHHHHHHHHHHHH	33.66	23806337
788	Malonylation	VKQLLTLKAEYKEKT HHHHHHHHHHHHHHH	33.66	26320211
795	Phosphorylation	KAEYKEKTGQEYKPG HHHHHHHHCCCCCCC	45.23	26487105
799	Phosphorylation	KEKTGQEYKPGNPSA HHHHCCCCCCCCCCC	18.00	26487105
800	Acetylation	EKTGQEYKPGNPSAA HHHCCCCCCCCCCCC	45.77	23236377
800	Malonylation	EKTGQEYKPGNPSAA HHHCCCCCCCCCCCC	45.77	26320211
813	Phosphorylation	AAAVQTVSTKSSSNT CCEEEEEECCCCCCC	33.24	29514104
815	Malonylation	AVQTVSTKSSSNTVE EEEEEECCCCCCCEE	40.61	26320211
823	Phosphorylation	SSSNTVESTSLYNKV CCCCCEEEHHHHHHH	20.96	29176673
824	Phosphorylation	SSNTVESTSLYNKVA CCCCEEEHHHHHHHH	14.83	29176673
825	Phosphorylation	SNTVESTSLYNKVAA CCCEEEHHHHHHHHH	37.83	29176673
829	Ubiquitination	ESTSLYNKVAAQGEV EEHHHHHHHHHCCHH	21.50	22790023
841	Acetylation	GEVVRKLKAEKAPKA CHHHHHHHHHCCCCC	58.49	132891
852	Ubiquitination	APKAKVTEAVECLLS CCCCHHHHHHHHHHH	54.42	27667366
859	Ubiquitination	EAVECLLSLKAEYKE HHHHHHHHCCHHHHH	18.18	27667366
861	Acetylation	VECLLSLKAEYKEKT HHHHHHCCHHHHHHC	35.48	23806337
863	Ubiquitination	CLLSLKAEYKEKTGK HHHHCCHHHHHHCCC	56.39	27667366
872	Phosphorylation	KEKTGKDYVPGQPPA HHHCCCCCCCCCCCC	16.42	-
885	Phosphorylation	PASQNSHSNPVSNAQ CCCCCCCCCCCCCCC	42.52	-
910	Glutathionylation	VLFDRVACQGEVVRK EEEEHHHHCHHHHHH	4.96	24333276
910	S-palmitoylation	VLFDRVACQGEVVRK EEEEHHHHCHHHHHH	4.96	28526873
919	Acetylation	GEVVRKLKAEKASKD HHHHHHHHHHHCCHH	58.49	8275869
925	Ubiquitination	LKAEKASKDQVDSAV HHHHHCCHHHHHHHH	57.97	22790023
939	Acetylation	VQELLQLKAQYKSLT HHHHHHHHHHHHHCC	22.08	23236377
939	Ubiquitination	VQELLQLKAQYKSLT HHHHHHHHHHHHHCC	22.08	22790023
942	Phosphorylation	LLQLKAQYKSLTGIE HHHHHHHHHHCCCCC	13.85	-
943	Malonylation	LQLKAQYKSLTGIEY HHHHHHHHHCCCCCE	27.23	26320211
944	Phosphorylation	QLKAQYKSLTGIEYK HHHHHHHHCCCCCEE	26.65	26824392
946	Phosphorylation	KAQYKSLTGIEYKPV HHHHHHCCCCCEECC	43.11	25162660
950	Phosphorylation	KSLTGIEYKPVSATG HHCCCCCEECCCCCC	20.76	28066266
951	Acetylation	SLTGIEYKPVSATGA HCCCCCEECCCCCCC	26.59	23954790
951	Malonylation	SLTGIEYKPVSATGA HCCCCCEECCCCCCC	26.59	26320211
951	Ubiquitination	SLTGIEYKPVSATGA HCCCCCEECCCCCCC	26.59	27667366
954	Phosphorylation	GIEYKPVSATGAEDK CCCEECCCCCCCCCH	28.74	28066266
956	Phosphorylation	EYKPVSATGAEDKDK CEECCCCCCCCCHHH	29.44	28066266
958	Ubiquitination	KPVSATGAEDKDKKK ECCCCCCCCCHHHHH	19.91	27667366
961	Acetylation	SATGAEDKDKKKKEK CCCCCCCHHHHHHHH	64.05	23236377
992	Phosphorylation	QGKDSSKSQGSGLSS CCCCCCCCCCCCCCC	41.54	29899451
995	Phosphorylation	DSSKSQGSGLSSGGA CCCCCCCCCCCCCCC	28.99	28066266
998	Phosphorylation	KSQGSGLSSGGAGEG CCCCCCCCCCCCCCC	30.38	28066266
999	Phosphorylation	SQGSGLSSGGAGEGQ CCCCCCCCCCCCCCC	46.41	25521595
1009	Ubiquitination	AGEGQGPKKQTRLGL CCCCCCCHHHCCCCC	65.42	22790023
1076	S-palmitoylation	KKLGVENCYFPIFVS HHHCCCCCCHHHHCC	2.04	28526873
1091	Ubiquitination	QAALEKEKNHIEDFA HHHHHHHHHCHHHHH	65.96	22790023
1109	Ubiquitination	AWVTRSGKTELAEPI HEEECCCCCCCCCCE	39.82	22790023
1116	Ubiquitination	KTELAEPIAIRPTSE CCCCCCCEEECCCCC	3.61	27667366
1138	Ubiquitination	AKWVQSHRDLPVRLN HHHHHHCCCCCCHHH	53.02	27667366
1145	Ubiquitination	RDLPVRLNQWCNVVR CCCCCHHHHHHCEEE	23.81	27667366
1148	Glutathionylation	PVRLNQWCNVVRWEF CCHHHHHHCEEEEEE	1.67	24333276
1148	S-palmitoylation	PVRLNQWCNVVRWEF CCHHHHHHCEEEEEE	1.67	28526873
1152	Methylation	NQWCNVVRWEFKHPQ HHHHCEEEEEECCCC	23.95	-
1156	Ubiquitination	NVVRWEFKHPQPFLR CEEEEEECCCCCCCC	42.75	22790023
1250	Acetylation	HLGQNFSKMCEIVFE HCCCCHHHHHEEEEC	43.35	22826441
1349	Phosphorylation	RVDLRDNYSPGWKFN EEECCCCCCCCCCCC	22.11	21743459
1350	Phosphorylation	VDLRDNYSPGWKFNH EECCCCCCCCCCCCC	24.16	21743459
1375	Malonylation	EVGPRDMKSCQFVAV EECCCCCCCCEEEEE	52.98	26320211
1375	Ubiquitination	EVGPRDMKSCQFVAV EECCCCCCCCEEEEE	52.98	-
1377	Glutathionylation	GPRDMKSCQFVAVRR CCCCCCCCEEEEEEC	2.80	24333276
1377	S-nitrosylation	GPRDMKSCQFVAVRR CCCCCCCCEEEEEEC	2.80	24926564
1377	S-palmitoylation	GPRDMKSCQFVAVRR CCCCCCCCEEEEEEC	2.80	26165157
1389	Acetylation	VRRDTGEKLTIAEKE EECCCCCCEEEHHHH	52.88	23806337
1395	Acetylation	EKLTIAEKEAEAKLE CCEEEHHHHHHHHHH	53.88	23806337
1395	Malonylation	EKLTIAEKEAEAKLE CCEEEHHHHHHHHHH	53.88	26320211
1395	Ubiquitination	EKLTIAEKEAEAKLE CCEEEHHHHHHHHHH	53.88	27667366
1402	Ubiquitination	KEAEAKLEKVLEDIQ HHHHHHHHHHHHHHH	40.17	27667366
1403	Ubiquitination	EAEAKLEKVLEDIQL HHHHHHHHHHHHHHH	63.66	22790023
1414	Phosphorylation	DIQLNLFTRASEDLK HHHHHHHHHCCHHHH	28.61	20047950
1435	Acetylation	NTLEDFQKVLDAGKV CCHHHHHHHHHCCCC	44.77	23954790
1435	Ubiquitination	NTLEDFQKVLDAGKV CCHHHHHHHHHCCCC	44.77	22790023
1473	Phosphorylation	DVEPGAPSMGAKSLC CCCCCCCCCCCCEEE	29.34	25521595
1487	Glutathionylation	CIPFNPLCELQPGAM EEECCCCCCCCCCCE	5.31	24333276
1503	Acetylation	VCGKNPAKFYTLFGR EECCCHHHHHHCCCC	39.91	22826441
1511	Phosphorylation	FYTLFGRSY------ HHHCCCCCC------	35.26	23984901
1512	Phosphorylation	YTLFGRSY------- HHCCCCCC-------	22.63	26643407

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
999	S	Phosphorylation	Kinase	RPS6KB1	Q8BSK8	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
999	S	Phosphorylation	23071094
In monocytes, the IFN-gamma-induced phosphorylation at Ser-999 releases EPRS1 from the aminoacyl-tRNA synthetase multienzyme complex, allowing its association with the GAIT complex.
999	S	Phosphorylation	23071094
Phosphorylated at Ser-999 by RPS6KB1; triggers EPRS1 release from the aminoacyl-tRNA synthetase multienzyme complex.
999	S	Phosphorylation	23071094
Phosphorylation at Ser-999 by RPS6KB1, is also induced by insulin through activation of the mTORC1 signaling pathway and promotes the interaction of EPRS1 with SLC27A1.
999	S	Phosphorylation	23071094
Phosphorylation at Ser-999 is specifically required for the RPL13A-mediated interaction of the GAIT complex with eIF4G (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SYEP_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of SYEP_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SYEP_MOUSE

Related Literatures of Post-Translational Modification

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