SLAP2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SLAP2_MOUSE
• UniProt AC: Q8R4L0
• Protein Name: Src-like-adapter 2
• Gene Name: Sla2
• Organism: Mus musculus (Mouse).
• Sequence Length: 259
• Subcellular Localization: Cytoplasm. Cell membrane. Cytoplasmic vesicle. Late endosome. Localized to the plasma membrane and intracellular vesicles, including late endosomal vesicles.
• Protein Description: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins..
• Protein Sequence: MGSLSSRGKTSSPSPSSSGPDQEPVSMQPERHKVTAVALGSFPAGEQARLSLRLGEPLTIISEDGDWWTVQSEVSGREYHMPSVYVAKVAHGWLYEGLSREKAEELLLLPGNPGGAFLIRESQTRRGCYSLSVRLSRPASWDRIRHYRIQRLDNGWLYISPRLTFPSLHALVEHYSELADGICCPLREPCVLQKLGPLPGKDTPPPVTVPTSSLNWKKLDRSLLFLEAPASGEASLLSEGLRESLSSYISLAEDPLDDA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	N-myristoyl glycine	------MGSLSSRGK ------CCCCCCCCC	36.98	-
2	Myristoylation	------MGSLSSRGK ------CCCCCCCCC	36.98	11891219
12	Phosphorylation	SSRGKTSSPSPSSSG CCCCCCCCCCCCCCC	34.33	30482847
16	Phosphorylation	KTSSPSPSSSGPDQE CCCCCCCCCCCCCCC	41.28	30482847
51	Phosphorylation	AGEQARLSLRLGEPL HHHHHHHEEECCCCE	13.09	24704852
136	Phosphorylation	YSLSVRLSRPASWDR EEEEEECCCCCCHHH	26.17	23140645
201	Acetylation	KLGPLPGKDTPPPVT CCCCCCCCCCCCCCC	57.39	19861615
201	Ubiquitination	KLGPLPGKDTPPPVT CCCCCCCCCCCCCCC	57.39	-
203	Phosphorylation	GPLPGKDTPPPVTVP CCCCCCCCCCCCCCC	40.98	25266776
208	Phosphorylation	KDTPPPVTVPTSSLN CCCCCCCCCCCCCCC	26.92	26745281
211	Phosphorylation	PPPVTVPTSSLNWKK CCCCCCCCCCCCCEE	26.98	26745281
212	Phosphorylation	PPVTVPTSSLNWKKL CCCCCCCCCCCCEEC	27.10	26745281
213	Phosphorylation	PVTVPTSSLNWKKLD CCCCCCCCCCCEECC	28.35	21082442

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SLAP2_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SLAP2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SLAP2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ZAP70_MOUSE	Zap70	physical	12024036
CBL_MOUSE	Cbl	physical	12024036

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Myristoylation

"A novel Src homology 2 domain-containing molecule, Src-like adapterprotein-2 (SLAP-2), which negatively regulates T cell receptorsignaling.";
Pandey A., Ibarrola N., Kratchmarova I., Fernandez M.M.,Constantinescu S.N., Ohara O., Sawasdikosol S., Lodish H.F., Mann M.;
J. Biol. Chem. 277:19131-19138(2002).
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION,MYRISTOYLATION AT GLY-2, INTERACTION WITH CBL; ZAP70 AND CD3Z, ANDMUTAGENESIS OF GLY-2; PRO-82 AND ARG-120.
PubMed: 11891219

"Functional cooperation between c-Cbl and Src-like adaptor protein 2in the negative regulation of T-cell receptor signaling.";
Loreto M.P., Berry D.M., McGlade C.J.;
Mol. Cell. Biol. 22:4241-4255(2002).
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE INITIATION,CHARACTERIZATION, FUNCTION, MYRISTOYLATION AT GLY-2, PHOSPHORYLATION,INTERACTION WITH ZAP70 AND CBL, AND MUTAGENESIS OF GLY-2; MET-27 ANDARG-120.
PubMed: 12024036