dbPTM

SKA3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SKA3_MOUSE
UniProt AC	Q8C263
Protein Name	Spindle and kinetochore-associated protein 3
Gene Name	Ska3
Organism	Mus musculus (Mouse).
Sequence Length	411
Subcellular Localization	Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner.
Protein Description	Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules..
Protein Sequence	MNPIQSFHCKLRGLATTLDSETARLLRALDGEDSDFEDSPGRILHDLHSEVQTLKDNVNALLDEARLENQESTRFKKATKILMEKNSADVRKLREFFQKYGYQARDKEDSGCEHRVNNSTPELAVCKDIQKAGVKELSDPCVPSGSVSEEPLRSPQLSDFGLQRYIISQVPANPPQTAASLKEERVAETPPAKDPSVQVLKTPRCALRMDDFECETPKLEHFGISEHTMCLNEDYTMGLKNMKNIKSSLLSGVSGEAIGTGPVTSDNSFAIPGPIIQQMEENDVEYVSSPLPPKFCTPGLKIPSTMDRTDLVSIDYPLSKPNSSSTDLEIKDCVPLILNSDECYQSFAEPPSSAITSCENFATPSPPKVTAIPEDILQMITKHSSNLASPLDVKVMPRRKGTRGAANKENW

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
34	Phosphorylation	RALDGEDSDFEDSPG HHHCCCCCCCCCCCC	39.64	26239621
39	Phosphorylation	EDSDFEDSPGRILHD CCCCCCCCCCHHHHH	23.27	28066266
79	Phosphorylation	STRFKKATKILMEKN HHHHHHHHHHHHHHC	27.74	17622165
119	Phosphorylation	CEHRVNNSTPELAVC CCCCCCCCCCCCCCC	39.89	25619855
120	Phosphorylation	EHRVNNSTPELAVCK CCCCCCCCCCCCCCH	23.94	26824392
138	Phosphorylation	KAGVKELSDPCVPSG HHCCCCCCCCCCCCC	40.37	21149613
144	Phosphorylation	LSDPCVPSGSVSEEP CCCCCCCCCCCCCCC	24.25	21149613
146	Phosphorylation	DPCVPSGSVSEEPLR CCCCCCCCCCCCCCC	27.14	21149613
148	Phosphorylation	CVPSGSVSEEPLRSP CCCCCCCCCCCCCCC	37.63	21149613
154	Phosphorylation	VSEEPLRSPQLSDFG CCCCCCCCCCHHHHC	25.36	26824392
158	Phosphorylation	PLRSPQLSDFGLQRY CCCCCCHHHHCCCHH	26.16	21149613
165	Phosphorylation	SDFGLQRYIISQVPA HHHCCCHHHHCCCCC	6.94	17242355
168	Phosphorylation	GLQRYIISQVPANPP CCCHHHHCCCCCCCC	18.63	17242355
189	Phosphorylation	KEERVAETPPAKDPS CHHHHCCCCCCCCCC	25.49	26239621
202	Phosphorylation	PSVQVLKTPRCALRM CCCEEECCCCCEEEC	16.29	28066266
288	Phosphorylation	ENDVEYVSSPLPPKF CCCCCEECCCCCCCC	25.90	29550500
289	Phosphorylation	NDVEYVSSPLPPKFC CCCCEECCCCCCCCC	22.03	19060867
297	Phosphorylation	PLPPKFCTPGLKIPS CCCCCCCCCCCCCCC	24.43	22006019
324	Phosphorylation	PLSKPNSSSTDLEIK CCCCCCCCCCCCCHH	43.85	27841257
389	Phosphorylation	KHSSNLASPLDVKVM HCCCCCCCCCCCEEC	29.21	26643407

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of SKA3_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SKA3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SKA3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of SKA3_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SKA3_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A differential phosphoproteomic analysis of retinoic acid-treated P19cells."; Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.; J. Proteome Res. 6:3174-3186(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-79, AND MASSSPECTROMETRY.	17622165 [Abstract]