dbPTM

SHAN2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SHAN2_MOUSE
UniProt AC	Q80Z38
Protein Name	SH3 and multiple ankyrin repeat domains protein 2
Gene Name	Shank2
Organism	Mus musculus (Mouse).
Sequence Length	1476
Subcellular Localization	Apical cell membrane. Cytoplasm . Cell junction, synapse . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell projection, dendritic spine . Cell projection, growth cone. Colocalizes with cortactin in growth cones in diffe
Protein Description	Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction (By similarity)..
Protein Sequence	MKSLLNAFTKKEVPFREAPAYSNRRRRPPNTLAAPRVLLRSNSDNNLNAGAPEWAVCSAATSHRSLSPQLLQQTPSKPDGATKSLGSYTPGPRSRSPSLNRLGGTAEDGKRTQPHWHVGSPFTPGANKDSLSTFEYPGPRRKLYSAVPGRLFVAVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDAASDCIIEDKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLILKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTAELEELGLSLVDKASVRKKKDKPEEIVPASKPSRTAENVAIESRVATIKQRPTSRCFPAASDVNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVVAKVPPATRSDTVATMMREKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYVPAKPARRKGVLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQATEPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQASKFPEEGGFGDEDETEQPLLPTPGAAPRELENHFLGGGEAGAQGEAGGPLSSTSKAKGPESGPAAPLKSSSPAGPENYVHPLTGRLLDPSSPLALALSARDRAMQESQQGHKGEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLGKDRRADDKKNMLINIVDTAQQKSAGLLMVHTVDVPMAGPPLEEEEDREDGDTKPDHSPSTVPEGVPKTEGALQISAAPEPAVAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEPLPPPLEFANSFDIPDDRAASVPALADLVKQKKNDTSQPPTLNSSQPANSTDSKKPAGISNCLPSSFLPPPESFDAVTDSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPKPKMKPIVHKSNALYQDTLPEEDTDGFVIPPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVPEVKSPILSGPKANVISELNSILQQMNRGKSVKPGEGLELPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGPRRAPSPVVSPTELSKEILPTPPPPSATAASPSPTLSDVFSLPSQSPAGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15	Phosphorylation	FTKKEVPFREAPAYS HHCCCCCCCCCCCCC	15.47	-
15 (in isoform 3)	Phosphorylation	-	15.47	22807455
17 (in isoform 3)	Phosphorylation	-	60.42	21183079
26 (in isoform 3)	Phosphorylation	-	52.82	27841257
41	Phosphorylation	APRVLLRSNSDNNLN CCEEEECCCCCCCCC	40.43	25521595
43	Phosphorylation	RVLLRSNSDNNLNAG EEEECCCCCCCCCCC	42.85	26643407
65	Phosphorylation	SAATSHRSLSPQLLQ HHHCCCCCCCHHHHH	27.98	26643407
67	Phosphorylation	ATSHRSLSPQLLQQT HCCCCCCCHHHHHCC	16.31	26239621
88	Phosphorylation	ATKSLGSYTPGPRSR CCCCCCCCCCCCCCC	18.37	23984901
89	Phosphorylation	TKSLGSYTPGPRSRS CCCCCCCCCCCCCCC	24.38	23984901
94	Phosphorylation	SYTPGPRSRSPSLNR CCCCCCCCCCCCCCC	40.00	29514104
96	Phosphorylation	TPGPRSRSPSLNRLG CCCCCCCCCCCCCCC	21.78	29899451
144	Phosphorylation	PGPRRKLYSAVPGRL CCCCHHHEEECCCCE	9.53	-
160	Phosphorylation	VAVKPYQPQVDGEIP EEECCCCCCCCCCCC	30.25	-
160 (in isoform 3)	Phosphorylation	-	30.25	22807455
162	Phosphorylation	VKPYQPQVDGEIPLH ECCCCCCCCCCCCCC	15.07	-
162 (in isoform 3)	Phosphorylation	-	15.07	25521595
164 (in isoform 3)	Phosphorylation	-	35.56	22324799
175 (in isoform 3)	Phosphorylation	-	38.05	29899451
227	Phosphorylation	SKKLFRHYTVGSYDS HHHHHHHCCCCCCCC	10.00	29899451
228	Phosphorylation	KKLFRHYTVGSYDSF HHHHHHCCCCCCCCC	16.56	29899451
231	Phosphorylation	FRHYTVGSYDSFDAA HHHCCCCCCCCCCCH	22.79	29899451
232	Phosphorylation	RHYTVGSYDSFDAAS HHCCCCCCCCCCCHH	15.07	29899451
234	Phosphorylation	YTVGSYDSFDAASDC CCCCCCCCCCCHHCE	19.45	19060867
363	Phosphorylation	PPPKRAPTTALTLRS CCCCCCCCCEEHHCC	24.58	20415495
367	Phosphorylation	RAPTTALTLRSKSMT CCCCCEEHHCCCCCC	20.05	20415495
370	Phosphorylation	TTALTLRSKSMTAEL CCEEHHCCCCCCHHH	31.99	19060867
370 (in isoform 2)	Phosphorylation	-	31.99	22807455
372	Phosphorylation	ALTLRSKSMTAELEE EEHHCCCCCCHHHHH	23.70	19060867
372 (in isoform 2)	Phosphorylation	-	23.70	25521595
374	Phosphorylation	TLRSKSMTAELEELG HHCCCCCCHHHHHHC	24.87	19060867
374 (in isoform 2)	Phosphorylation	-	24.87	22324799
385 (in isoform 2)	Phosphorylation	-	6.79	29899451
404	O-linked_Glycosylation	PEEIVPASKPSRTAE CHHHCCCCCCCCCHH	38.74	30059200
427	O-linked_Glycosylation	ATIKQRPTSRCFPAA EECCCCCCCCCCCCH	30.87	30059200
450	Phosphorylation	RQGIAVMTPTVPGSP HCCEEEECCCCCCCC	14.81	28066266
452	Phosphorylation	GIAVMTPTVPGSPKG CEEEECCCCCCCCCC	30.11	26643407
456	Phosphorylation	MTPTVPGSPKGPFLG ECCCCCCCCCCCCCC	19.85	25521595
474	Phosphorylation	GTMRRQKSIDSRIFL CCCCCCCCCCHHHHH	24.24	22324799
477	Phosphorylation	RRQKSIDSRIFLSGI CCCCCCCHHHHHCCC	26.13	22324799
482	Phosphorylation	IDSRIFLSGITEEER CCHHHHHCCCCHHHH	19.87	22324799
485	O-linked_Glycosylation	RIFLSGITEEERQFL HHHHCCCCHHHHHHH	40.65	145269
485	Phosphorylation	RIFLSGITEEERQFL HHHHCCCCHHHHHHH	40.65	22324799
502	Phosphorylation	PMLKFTRSLSMPDTS CCHHHHCCCCCCCCC	23.03	19060867
509	Phosphorylation	SLSMPDTSEDIPPPP CCCCCCCCCCCCCCC	40.42	24759943
518	Phosphorylation	DIPPPPQSVPPSPPP CCCCCCCCCCCCCCC	42.22	21183079
522	Phosphorylation	PPQSVPPSPPPPSPT CCCCCCCCCCCCCCC	43.92	22807455
529	Phosphorylation	SPPPPSPTTYNCPRS CCCCCCCCCCCCCCC	46.37	28576409
542	Phosphorylation	RSPTPRVYGTIKPAF CCCCCCCCCEECCHH	15.16	29899451
562	O-linked_Glycosylation	VAKVPPATRSDTVAT EEECCCCCCCHHHHH	36.16	30059200
566	Phosphorylation	PPATRSDTVATMMRE CCCCCCHHHHHHHHH	17.18	25521595
586	Phosphorylation	RRELDRFSLDSEDVY EHHCCCCCCCCHHCC	31.96	25521595
589	Phosphorylation	LDRFSLDSEDVYSRS CCCCCCCCHHCCCCC	40.78	26239621
593	Phosphorylation	SLDSEDVYSRSPAPQ CCCCHHCCCCCCCCH	15.64	24925903
594	Phosphorylation	LDSEDVYSRSPAPQA CCCHHCCCCCCCCHH	26.57	24925903
615	Phosphorylation	GQMPENPYSEVGKIA CCCCCCCHHHHHHHC	29.10	29899451
616	Phosphorylation	QMPENPYSEVGKIAS CCCCCCHHHHHHHCC	27.36	26643407
643	Phosphorylation	KGVLVKQSNVEDSPE CCEEEECCCCCCCCC	36.81	20415495
648	Phosphorylation	KQSNVEDSPEKTCSI ECCCCCCCCCCCCCC	22.81	25521595
652	Phosphorylation	VEDSPEKTCSIPIPT CCCCCCCCCCCCCCE	15.15	20415495
654	Phosphorylation	DSPEKTCSIPIPTII CCCCCCCCCCCCEEE	36.31	20415495
659	Phosphorylation	TCSIPIPTIIVKEPS CCCCCCCEEEEECCC	24.85	20415495
668	Phosphorylation	IVKEPSTSSSGKSSQ EEECCCCCCCCCCCC	27.18	29899451
673	Phosphorylation	STSSSGKSSQGSSME CCCCCCCCCCCCCCE	31.27	29899451
674	Phosphorylation	TSSSGKSSQGSSMEI CCCCCCCCCCCCCEE	41.89	29899451
678	Phosphorylation	GKSSQGSSMEIDPQA CCCCCCCCCEECCCC	27.53	29899451
724	Phosphorylation	RLEARRNSPAFLSTD HHHHHHCCCCCCCCC	18.78	24925903
729	Phosphorylation	RNSPAFLSTDLGDED HCCCCCCCCCCCCCC	17.27	25521595
730	Phosphorylation	NSPAFLSTDLGDEDV CCCCCCCCCCCCCCC	37.07	25521595
817	Phosphorylation	GPAAPLKSSSPAGPE CCCCCCCCCCCCCCC	43.67	26643407
818	Phosphorylation	PAAPLKSSSPAGPEN CCCCCCCCCCCCCCC	38.46	26643407
819	Phosphorylation	AAPLKSSSPAGPENY CCCCCCCCCCCCCCC	26.34	26643407
838	Phosphorylation	TGRLLDPSSPLALAL CCCCCCCCCHHHHHH	44.23	-
839	Phosphorylation	GRLLDPSSPLALALS CCCCCCCCHHHHHHH	29.35	-
881	Phosphorylation	IDTKMRPSVESGFPP EECCCCCCHHHCCCC	28.31	21189417
884	Phosphorylation	KMRPSVESGFPPVTR CCCCCHHHCCCCCCC	43.51	24759943
890	O-linked_Glycosylation	ESGFPPVTRQNTRGP HHCCCCCCCCCCCCC	32.10	55413759
890	Phosphorylation	ESGFPPVTRQNTRGP HHCCCCCCCCCCCCC	32.10	-
894	Phosphorylation	PPVTRQNTRGPLRRQ CCCCCCCCCCCCCHH	28.76	-
903	Phosphorylation	GPLRRQETENKYETD CCCCHHHHCCHHCCC	36.20	25521595
907	Phosphorylation	RQETENKYETDLGKD HHHHCCHHCCCCCCC	35.33	25521595
980	O-linked_Glycosylation	VPEGVPKTEGALQIS CCCCCCCCCCCEEEE	33.31	30059200
999	Phosphorylation	PAVAPGRTIVAAGSV CCCCCCCEEEECCCC	26.22	-
1005	Phosphorylation	RTIVAAGSVEEAVIL CEEEECCCCEEEEEE	22.94	18056256
1055	Phosphorylation	IPDDRAASVPALADL CCCCHHHHHHHHHHH	27.87	29899451
1107	Phosphorylation	SFLPPPESFDAVTDS HHCCCCHHHCCCCCC	33.91	29899451
1112	Phosphorylation	PESFDAVTDSGIEEV CHHHCCCCCCCCCCC	26.60	29899451
1191	Phosphorylation	SNALYQDTLPEEDTD CCCCCCCCCCCCCCC	28.78	24759943
1226	Phosphorylation	AKVIQPRTSKLWGDV EEHHCCCCHHHCCCC	36.58	21082442
1227	Phosphorylation	KVIQPRTSKLWGDVP EHHCCCCHHHCCCCC	27.34	22807455
1238	Phosphorylation	GDVPEVKSPILSGPK CCCCCCCCCHHCCCC	23.50	29899451
1242	Phosphorylation	EVKSPILSGPKANVI CCCCCHHCCCCHHHH	54.49	23984901
1264	Phosphorylation	QQMNRGKSVKPGEGL HHHHCCCCCCCCCCC	37.89	25521595
1286	Phosphorylation	SANLAPRSPEVMSTV CCCCCCCCHHHHHEE	25.09	28066266
1291	O-linked_Glycosylation	PRSPEVMSTVSGTRS CCCHHHHHEECCCCE	30.72	8522215
1291	Phosphorylation	PRSPEVMSTVSGTRS CCCHHHHHEECCCCE	30.72	-
1292	O-linked_Glycosylation	RSPEVMSTVSGTRST CCHHHHHEECCCCEE	10.70	30059200
1292	Phosphorylation	RSPEVMSTVSGTRST CCHHHHHEECCCCEE	10.70	-
1294	O-linked_Glycosylation	PEVMSTVSGTRSTTV HHHHHEECCCCEEEE	34.24	8522223
1294	Phosphorylation	PEVMSTVSGTRSTTV HHHHHEECCCCEEEE	34.24	-
1296	O-linked_Glycosylation	VMSTVSGTRSTTVTF HHHEECCCCEEEEEE	17.59	8522207
1296	Phosphorylation	VMSTVSGTRSTTVTF HHHEECCCCEEEEEE	17.59	-
1302	O-linked_Glycosylation	GTRSTTVTFTVRPGT CCCEEEEEEEECCCC	16.11	30059200
1334	Phosphorylation	SGPRRAPSPVVSPTE CCCCCCCCCCCCHHH	29.37	25521595
1338	Phosphorylation	RAPSPVVSPTELSKE CCCCCCCCHHHHHHC	26.74	25521595
1340	Phosphorylation	PSPVVSPTELSKEIL CCCCCCHHHHHHCCC	41.89	25521595
1343	Phosphorylation	VVSPTELSKEILPTP CCCHHHHHHCCCCCC	23.24	24925903
1359	Phosphorylation	PPSATAASPSPTLSD CCCCCCCCCCCCHHH	24.36	29899451
1361	Phosphorylation	SATAASPSPTLSDVF CCCCCCCCCCHHHHH	28.20	29899451
1365	Phosphorylation	ASPSPTLSDVFSLPS CCCCCCHHHHHCCCC	33.92	29899451
1388	Phosphorylation	GLNPAGRSRSPSPSI CCCCCCCCCCCCCCH	35.87	21743459
1390	Phosphorylation	NPAGRSRSPSPSILQ CCCCCCCCCCCCHHC	30.86	21743459
1392	Phosphorylation	AGRSRSPSPSILQQP CCCCCCCCCCHHCCC	32.31	21743459
1394	Phosphorylation	RSRSPSPSILQQPIS CCCCCCCCHHCCCCC	40.02	21743459
1401	Phosphorylation	SILQQPISNKPFTTK CHHCCCCCCCCCCCC	45.55	20469934
1468	Methylation	GHRMNIERALKQLLD HHHHCHHHHHHHHHC	41.23	18959257

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of SHAN2_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SHAN2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SHAN2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of SHAN2_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SHAN2_MOUSE

Related Literatures of Post-Translational Modification

O-linked Glycosylation
Reference	PubMed
"O-linked N-acetylglucosamine proteomics of postsynaptic densitypreparations using lectin weak affinity chromatography and massspectrometry."; Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G.,Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F.,Maltby D.A., Schoepfer R., Burlingame A.L.; Mol. Cell. Proteomics 5:923-934(2006). Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-1292, AND MASSSPECTROMETRY.	16452088 [Abstract]
Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1334 AND SER-1338, ANDMASS SPECTROMETRY.	17242355 [Abstract]
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586; THR-730; THR-903AND SER-1338, AND MASS SPECTROMETRY.	16452087 [Abstract]
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1340, AND MASSSPECTROMETRY.	17114649 [Abstract]