SCN9A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SCN9A_MOUSE
• UniProt AC: Q62205
• Protein Name: Sodium channel protein type 9 subunit alpha {ECO:0000250|UniProtKB:Q15858}
• Gene Name: Scn9a {ECO:0000312|MGI:MGI:107636}
• Organism: Mus musculus (Mouse).
• Sequence Length: 1984
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Cell projection . In neurite terminals.
• Protein Description: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. [PubMed: 15123669 It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain]
• Protein Sequence: MAMLPPPGPQSFVHFTKQSLALIEQRISEEKAKGHKDEKKDDEEEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCFRKDLEQNETLESIMSTAESEEELKRYFYYLEGSKDALLCGFSTDSGQCPEGYECVTAGRNPDYGYTSFDTFGWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEAKQKELEFQQMLDRLKKEQEEAEAIAAAAAEYTSLGRSRIMGLSESSSETSRLSSKSAKERRNRRKKKKQKLSSGEEKGDDEKLSKSGSEESIRKKSFHLGVEGHHRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDLGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQMRKKRLSSSYFLSEDMLNDPHLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTDEFKNVLAVGNLVFTGIFAAEMVLKLIAMDPYEYFQVGWNIFDSLIVTLSLVELFLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINENCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKRGINYVKQTLREFILKSFSKKPKGSKDTKRTADPNNKRENYISNRTLAEISKDHNFLKEKDKISGFSSSLDKSFMDENDYQSFIHNPSLTVTVPIAPGESDLENMNTEELSSDSDSDYSKERRNRSSSSECSTVDNPLPGEEEAEAEPINADEPEACFTDGCVRRFPCCQVNIDSGKGKVWWTIRKTCYRIVEHSWFESFIVLMILLSSGALAFEDIYIEKKKTIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECVNTTDGSRFSVSQVANRSECFALMNVSGNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVNAQPIYEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDITIMVLICLNMVTMMVEKEGQTDYMSFVLYWINVVFIILFTGECVLKLISLRHYYFTVGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSAPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATIIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDIVFDNVNENSSPEKTDATASTISPPSYDSVTKPDQEKYETDKTEKEDKEKDESRK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
86	Ubiquitination	DPYYADKKTFIVLNK CCCCCCCCEEEEEEC	49.57	-
302	Phosphorylation	SEEELKRYFYYLEGS CHHHHHHHHHHHHCC	8.37	-
304	Phosphorylation	EELKRYFYYLEGSKD HHHHHHHHHHHCCCC	9.75	-
305	Phosphorylation	ELKRYFYYLEGSKDA HHHHHHHHHHCCCCE	6.59	-
309	Phosphorylation	YFYYLEGSKDALLCG HHHHHHCCCCEEEEC	20.28	-
447	Phosphorylation	IAAAAAEYTSLGRSR HHHHHHHHHHHCCHH	9.26	29899451
448	Phosphorylation	AAAAAEYTSLGRSRI HHHHHHHHHHCCHHH	14.69	28066266
449	Phosphorylation	AAAAEYTSLGRSRIM HHHHHHHHHCCHHHC	28.11	28066266
453	Phosphorylation	EYTSLGRSRIMGLSE HHHHHCCHHHCCCCC	25.10	29899451
502	Phosphorylation	DDEKLSKSGSEESIR CHHHHHCCCCHHHHH	44.14	20415495
504	Phosphorylation	EKLSKSGSEESIRKK HHHHCCCCHHHHHHH	45.33	20415495
507	Phosphorylation	SKSGSEESIRKKSFH HCCCCHHHHHHHHHC	24.52	20415495
512	Phosphorylation	EESIRKKSFHLGVEG HHHHHHHHHCCCCCC	22.42	29899451
529	Phosphorylation	RAREKRLSTPNQSPL HHHHHCCCCCCCCCC	45.94	28066266
530	Phosphorylation	AREKRLSTPNQSPLS HHHHCCCCCCCCCCH	30.74	28066266
534	Phosphorylation	RLSTPNQSPLSIRGS CCCCCCCCCCHHCCC	34.15	28066266
537	Phosphorylation	TPNQSPLSIRGSLFS CCCCCCCHHCCCHHH	17.39	28066266
551	Phosphorylation	SARRSSRTSLFSFKG HCCCCCCCCCEEECC	31.56	20139300
565	Phosphorylation	GRGRDLGSETEFADD CCCCCCCCCCCCCCC	48.95	28066266
567	Phosphorylation	GRDLGSETEFADDEH CCCCCCCCCCCCCCC	37.79	28066266
598	Phosphorylation	HRPRERRSSNISQAS CCCCCCCCCCCCHHC	33.68	29899451
599	Phosphorylation	RPRERRSSNISQASR CCCCCCCCCCCHHCC	36.05	29899451
605	Phosphorylation	SSNISQASRSPPVLP CCCCCHHCCCCCEEC	26.17	29899451
669	Phosphorylation	QMRKKRLSSSYFLSE HHHHHHHCCHHCCCH	22.67	29899451
670	Phosphorylation	MRKKRLSSSYFLSED HHHHHHCCHHCCCHH	33.38	29899451
671	Phosphorylation	RKKRLSSSYFLSEDM HHHHHCCHHCCCHHH	19.27	29899451
672	Phosphorylation	KKRLSSSYFLSEDML HHHHCCHHCCCHHHH	15.53	29899451
675	Phosphorylation	LSSSYFLSEDMLNDP HCCHHCCCHHHHCCH	22.96	-
690	Phosphorylation	HLRQRAMSRASILTN HHHHHHHHHHHHHHH	24.58	-
693	Phosphorylation	QRAMSRASILTNTVE HHHHHHHHHHHHHHH	19.61	28066266
696	Phosphorylation	MSRASILTNTVEELE HHHHHHHHHHHHHHH	27.46	28066266
698	Phosphorylation	RASILTNTVEELEES HHHHHHHHHHHHHHH	24.86	28066266
705	Phosphorylation	TVEELEESRQKCPPW HHHHHHHHHHHCCCH	30.54	28066266
968	Phosphorylation	LFLALLLSSFSSDNL HHHHHHHHHCCCCCC	29.34	-
1062	Phosphorylation	KDKISGFSSSLDKSF HHCCCCCCCCCCHHH	23.65	28066266
1063	Phosphorylation	DKISGFSSSLDKSFM HCCCCCCCCCCHHHC	32.43	28066266
1064	Phosphorylation	KISGFSSSLDKSFMD CCCCCCCCCCHHHCC	39.11	20415495
1075	Phosphorylation	SFMDENDYQSFIHNP HHCCCCCHHHHCCCC	20.03	25521595
1095	Phosphorylation	VPIAPGESDLENMNT EEECCCCHHHHCCCH	54.10	25521595
1484	Ubiquitination	KKYYNAMKKLGSKKP HHHHHHHHHHCCCCC	42.30	-
1488	Phosphorylation	NAMKKLGSKKPQKPI HHHHHHCCCCCCCCC	48.20	-
1867	Phosphorylation	QMEERFMSANPSKVS HHHHHHHCCCCCCCC	23.37	28059163
1874	Phosphorylation	SANPSKVSYEPITTT CCCCCCCCCCCCCHH	27.75	28059163
1883	Ubiquitination	EPITTTLKRKQEDVS CCCCHHHHHCHHCHH	56.30	-
1939	Phosphorylation	FDNVNENSSPEKTDA EECCCCCCCCCCCCC	40.88	23375375
1940	Phosphorylation	DNVNENSSPEKTDAT ECCCCCCCCCCCCCC	48.97	23375375

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1488	S	Phosphorylation	Kinase	PKC	-	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Nedd4l	Q8CFI0	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
1488	S	Phosphorylation	Phosphorylation at Ser-1488 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SCN9A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NEDD4_MOUSE	Nedd4	physical	15123669

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.