SCN1A_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SCN1A_RAT
• UniProt AC: P04774
• Protein Name: Sodium channel protein type 1 subunit alpha
• Gene Name: Scn1a
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 2009
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein .
• Protein Description: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. Plays a key role in brain, probably by regulating the moment when neurotransmitters are released in neurons. Involved in sensory perception of mechanical pain: activation in somatosensory neurons induces pain without neurogenic inflammation and produces hypersensitivity to mechanical, but not thermal stimuli..
• Protein Sequence: MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIRNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
211	N-linked_Glycosylation	TEFVDLGNVSALRTF EECCCCCCHHHHHHH	31.69	-
284	N-linked_Glycosylation	CVQWPPTNASLEEHS HCCCCCCCCCHHHHC	33.05	-
295	N-linked_Glycosylation	EEHSIEKNVTTDYNG HHHCCCCCCCCCCCC	24.47	-
301	N-linked_Glycosylation	KNVTTDYNGTLVNET CCCCCCCCCCEECEE	39.52	-
306	N-linked_Glycosylation	DYNGTLVNETVFEFD CCCCCEECEEEEEEC	42.19	-
338	N-linked_Glycosylation	LDALLCGNSSDAGQC HHHHHCCCCCCCCCC	37.03	-
453	Ubiquitination	QMLEQLKKQQEAAQQ HHHHHHHHHHHHHHH	66.85	-
470	Phosphorylation	AATASEHSREPSAAG HHHHHHHCCCCCCCC	34.00	20131913
523	Phosphorylation	DDDEFHKSESEDSIR CCCCCCCCCCHHHHH	37.47	22673903
525	Phosphorylation	DEFHKSESEDSIRRK CCCCCCCCHHHHHHH	54.52	22673903
528	Phosphorylation	HKSESEDSIRRKGFR CCCCCHHHHHHHCEE	17.59	25403869
537	Phosphorylation	RRKGFRFSIEGNRLT HHHCEEEEEECCCCE	18.57	25403869
549	Phosphorylation	RLTYEKRYSSPHQSL CCEEEECCCCCCHHH	25.27	22673903
550	Phosphorylation	LTYEKRYSSPHQSLL CEEEECCCCCCHHHH	41.23	22673903
551	Phosphorylation	TYEKRYSSPHQSLLS EEEECCCCCCHHHHH	19.91	22673903
565	Phosphorylation	SIRGSLFSPRRNSRT HHHCCCCCCCCCCCC	23.84	30411139
576	Phosphorylation	NSRTSLFSFRGRAKD CCCCCCEEECCCCCC	21.23	28432305
607	Phosphorylation	DNESRRDSLFVPRRH CCCHHHHCCCCCCCC	23.15	20131913
620	Phosphorylation	RHGERRNSNLSQTSR CCCCCCCCCCCCCCH	37.02	25403869
715	Phosphorylation	SQRQRAMSIASILTN HHHHHHHHHHHHHHH	17.64	22673903
718	Phosphorylation	QRAMSIASILTNTVE HHHHHHHHHHHHHHH	19.23	22673903
721	Phosphorylation	MSIASILTNTVEELE HHHHHHHHHHHHHHH	27.46	25403869
723	Phosphorylation	IASILTNTVEELEES HHHHHHHHHHHHHHH	24.86	22673903
730	Phosphorylation	TVEELEESRQKCPPC HHHHHHHHHHHCCCC	30.54	25403869
1378	N-linked_Glycosylation	GKFYHCVNTTTGDTF CEEEEEEECCCCCEE	36.78	-
1392	N-linked_Glycosylation	FEITEVNNHSDCLKL EEEEEECCHHHHHHH	41.01	-
1403	N-linked_Glycosylation	CLKLIERNETARWKN HHHHHHHCCCCCCCC	37.87	-
1439	Phosphorylation	KGWMDIMYAAVDSRN CCHHHHHHHHHHCCC	7.55	-
1507	Phosphorylation	MTEEQKKYYNAMKKL CCHHHHHHHHHHHHH	14.69	17959797
1508	Phosphorylation	TEEQKKYYNAMKKLG CHHHHHHHHHHHHHC	12.81	17959797
1516	Phosphorylation	NAMKKLGSKKPQKPI HHHHHHCCCCCCCCC	48.20	22426212
1939	Phosphorylation	KRTVKQASFTYNKNK HHHHHHHHCCCCCCC	18.67	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1516	S	Phosphorylation	Kinase	PKC	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
1516	S	Phosphorylation	Phosphorylation at Ser-1516 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SCN1A_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of SCN1A_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.