SCAP_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SCAP_MOUSE
• UniProt AC: Q6GQT6
• Protein Name: Sterol regulatory element-binding protein cleavage-activating protein
• Gene Name: Scap {ECO:0000312|MGI:MGI:2135958}
• Organism: Mus musculus (Mouse).
• Sequence Length: 1276
• Subcellular Localization: Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, COPII-coated vesicle membrane ; Multi-pass membrane protein . Moves from the endoplasmic reticulum to the Gol
• Protein Description: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway..
• Protein Sequence: MTLTERLREKISQAFYNHGLLCASYPIPIILFTGLCILACCYPLLKLPLPGTGPVEFSTPVKGYSPPPADSDHKQGEPSEQPEWYVGAPVAYIQQIFVKSSVSPWHRNLLAVDVFRSPLSRAFQLVEEIRNHVLRDSSGTKSLEEVCLQVTDLLPGLRKLRSLLPEHGCLLLSPGNFWQNDWERFHADPDIIGTIHQHEPKTLQTSATLKDLLFGVPGKYSGVSLYTRKRMVSYTITLVFQRYHAKFLSSLRARLMLLHPSPNCSLRAENLVHVHFKEEIGIAELIPLVTTYIILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFPYLVVVIGLENVLVLTKSVVSTPVDLEVKLRIAQGLSSESWSIMKNAATELGIILIGYFTLVPAIQEFCLFAVVGLVSDFFLQMLFFTTVLSIDIRRMELADLNKRLPPESCLPSAKPVGRPARYERQQAVRPSTPHTITLQPSSFRNLRLPKRLRVIYFLARTRLAQRLIMAGTVVWIGILVYTDPAGLRTYLAAQVTEQSPLGEGSLGPMPVPSGVLPASHPDPAFSIFPPDAPKLPENQTLPGELPEHAGPAEGVHDSRAPEVTWGPEDEELWRKLSFRHWPTLFNYYNITLAKRYISLLPVIPVTLHLNPREALEGRHPQDGRSAWAPQEPLPAGLWESGPKGPGGTQTHGDITLYKVAALGLAAGIVLVLLLLCLYRVLCPRNYGQPGGGPGRRRRGELPCDDYGYAPPETEIVPLVLRGHLMDIECLASDGMLLVSCCLAGQVCVWDAQTGDCLTRIPRPGPRRDSCGGGAFETQENWERLSDGGKASPEEPGDSPPLRRRPRGPPPPSLFGDQPDLTCLIDTNFSVQLPPEPTQPEPRHRVGCGRSRDSGYDFSRLVQRVYQEEGLAAMRMPALRPPSPGPPLPQASQEEGTAPEKGSPPLAWTPSTAGSIWSLELQGNLIVVGRSSGRLEVWDAIEGVLCCSNEEISSGITALVFLDRRIVAARLNGSLDFFSLETHTSLSPLQFRGTPGRGSSPSSSVYSSSNTVTCHRTHTVPCAHQKPITALRAAAGRLVTGSQDHTLRVFRLDDSCCLFTLKGHSGAITAVYIDQTMVLASGGQDGAICLWDVLTGSRVSQTFAHRGDVTSLTCTASCVISSGLDDFISIWDRSTGIKLYSIQQDLGCGASLGVISDNLLVTGGQGCVSFWDLNYGDLLQTVYLGKNSEAQPARQILVLDNAAIVCNFGSELSLVYVPSVLEKLD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
52	Phosphorylation	LKLPLPGTGPVEFST HCCCCCCCCCCCCCC	36.05	28059163
208	Phosphorylation	KTLQTSATLKDLLFG CCCCCCCCHHHHHHC	33.82	23140645
249	Phosphorylation	RYHAKFLSSLRARLM HHHHHHHHHHHHHHH	30.38	25159016
250	Phosphorylation	YHAKFLSSLRARLML HHHHHHHHHHHHHHH	24.83	25159016
263	N-linked_Glycosylation	MLLHPSPNCSLRAEN HHHCCCCCCCCCCCC	32.14	-
454	Ubiquitination	MELADLNKRLPPESC HHHHHHHHCCCHHHH	63.71	PubMed
466	Ubiquitination	ESCLPSAKPVGRPAR HHHCCCCCCCCCCCC	44.57	PubMed
483	Phosphorylation	RQQAVRPSTPHTITL HHHCCCCCCCCEEEE	43.96	23140645
484	Phosphorylation	QQAVRPSTPHTITLQ HHCCCCCCCCEEEEC	23.05	23140645
487	Phosphorylation	VRPSTPHTITLQPSS CCCCCCCEEEECCHH	19.50	22871156
590	N-linked_Glycosylation	DAPKLPENQTLPGEL CCCCCCCCCCCCCCC	37.89	-
641	N-linked_Glycosylation	PTLFNYYNITLAKRY CHHHHHCCHHHHHHH	15.48	-
730	Phosphorylation	VLLLLCLYRVLCPRN HHHHHHHHHHHCCCC	9.55	-
755	S-palmitoylation	RRRGELPCDDYGYAP CCCCCCCCCCCCCCC	10.99	28680068
821	Phosphorylation	RPGPRRDSCGGGAFE CCCCCCCCCCCCCCC	16.92	25521595
829	Phosphorylation	CGGGAFETQENWERL CCCCCCCCHHHHHHH	34.43	23984901
837	Phosphorylation	QENWERLSDGGKASP HHHHHHHCCCCCCCC	40.19	25159016
843	Phosphorylation	LSDGGKASPEEPGDS HCCCCCCCCCCCCCC	36.01	27818261
850	Phosphorylation	SPEEPGDSPPLRRRP CCCCCCCCCCCCCCC	34.04	25521595
905	Phosphorylation	GCGRSRDSGYDFSRL CCCCCCCCCCCHHHH	37.84	25266776
907	Phosphorylation	GRSRDSGYDFSRLVQ CCCCCCCCCHHHHHH	20.12	26643407
934	Phosphorylation	MPALRPPSPGPPLPQ CCCCCCCCCCCCCCC	45.21	26824392
943	Phosphorylation	GPPLPQASQEEGTAP CCCCCCCCCCCCCCC	32.64	25159016
948	Phosphorylation	QASQEEGTAPEKGSP CCCCCCCCCCCCCCC	42.17	26643407
1030	Phosphorylation	NGSLDFFSLETHTSL CCCCEEEEEEECCCC	25.86	26643407
1033	Phosphorylation	LDFFSLETHTSLSPL CEEEEEEECCCCCCE	35.43	26643407
1035	Phosphorylation	FFSLETHTSLSPLQF EEEEEECCCCCCEEE	38.49	26643407
1036	Phosphorylation	FSLETHTSLSPLQFR EEEEECCCCCCEEEC	21.17	26643407
1038	Phosphorylation	LETHTSLSPLQFRGT EEECCCCCCEEECCC	24.27	26643407
1045	Phosphorylation	SPLQFRGTPGRGSSP CCEEECCCCCCCCCC	20.50	26643407
1048	Methylation	QFRGTPGRGSSPSSS EECCCCCCCCCCCCC	42.92	24129315
1050	Phosphorylation	RGTPGRGSSPSSSVY CCCCCCCCCCCCCCC	37.40	25266776
1051	Phosphorylation	GTPGRGSSPSSSVYS CCCCCCCCCCCCCCC	31.46	25266776
1053	Phosphorylation	PGRGSSPSSSVYSSS CCCCCCCCCCCCCCC	36.87	26643407
1054	Phosphorylation	GRGSSPSSSVYSSSN CCCCCCCCCCCCCCC	27.49	26643407
1055	Phosphorylation	RGSSPSSSVYSSSNT CCCCCCCCCCCCCCE	29.43	26643407
1057	Phosphorylation	SSPSSSVYSSSNTVT CCCCCCCCCCCCEEE	12.56	26643407
1058	Phosphorylation	SPSSSVYSSSNTVTC CCCCCCCCCCCEEEE	25.74	26643407
1060	Phosphorylation	SSSVYSSSNTVTCHR CCCCCCCCCEEEEEE	30.49	26643407
1111	Phosphorylation	DDSCCLFTLKGHSGA CCCEEEEEECCCCCC	18.02	22871156

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SCAP_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
454	K	ubiquitylation	Ubiquitinated at Lys-454 and Lys-466.	29068315
466	K	ubiquitylation	Ubiquitinated at Lys-454 and Lys-466.	29068315

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SCAP_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
INSI1_HUMAN	INSIG1	physical	12202038
SRBP2_HUMAN	SREBF2	physical	12202038

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.