dbPTM

SCAF8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SCAF8_HUMAN
UniProt AC	Q9UPN6
Protein Name	Protein SCAF8
Gene Name	SCAF8
Organism	Homo sapiens (Human).
Sequence Length	1271
Subcellular Localization	Nucleus. Nucleus matrix. Detected in granular nuclear foci which correspond to sites of active transcription..
Protein Description	May play a role in mRNA processing..
Protein Sequence	MEAVKTFNSELYSLNDYKPPISKAKMTQITKAAIKAIKFYKHVVQSVEKFIQKCKPEYKVPGLYVIDSIVRQSRHQFGQEKDVFAPRFSNNIISTFQNLYRCPGDDKSKIVRVLNLWQKNNVFKSEIIQPLLDMAAGIPPPVVTPVLASTTTAMSNTPGTPVTPVTPANVVQGLPDPWVSQITNTDTLAAVAQILQSPQGQQLQQLIQTLQIQQQKPQPSILQALDAGLVVQLQALTAQLTAAAAAANTLTPLEQGVSFNKKLMDRFDFGEDSEHSEEPKKEIPASQLSHVSESVNNSIFHQIAEQLQQQNLEHLRQQLLEQQQPQKATPQDSQEGTFGSEHSASPSQGSSQQHFLEPEVNLDDSIDIQQQDMDIDEGQDGVEEEVFEQEAKKVAVRSRSRTHSRSRSRSPRKRRSRSRSGSRKRKHRKRSRSRSRERKRKSSRSYSSERRAREREKERQKKGLPPIRSKTLSVCSTTLWVGQVDKKATQQDLTNLFEEFGQIESINMIPPRGCAYVCMVHRQDAFRALQKLSSGSYKIGSKVIKIAWALNKGVKTEYKQFWDVDLGVTYIPWEKVKVDDLEGFAEGGMIDQETVNTEWETVKSSEPVKETVQTTQSPTPVEKETVVTTQAEVFPPPVAMLQIPVAPAVPTVSLVPPAFPVSMPVPPPGFSPIPPPPFLRASFNPSQPPPGFMPPPVPPPVVPPPTIPPVVPTSLVQPSLSMTPETVKDVGFGSLVIPGGSVASNLATSALPAGNVFNAPTKQAEPEEKVPHLIDHQISSGENTRSVIPNDISSNAAILGGQPPNVTSNSGILGVQRPNVSSNSEILGVRPSNVSSSSGIIAAQPPNILNNSGILGIQPPSVSNSSGLLGVLPPNIPNNSGLVGVQPPNVPNTPGLLGTQPPAGPQNLPPLSIPNQRMPTMPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFLAPGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPLDGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFRSGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHARVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVESTETEGT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MEAVKTFNSELYS --CCHHHHHCHHHHH	22.81	20388717
12	Phosphorylation	KTFNSELYSLNDYKP HHHCHHHHHCCCCCC	13.68	28152594
13	Phosphorylation	TFNSELYSLNDYKPP HHCHHHHHCCCCCCC	33.06	28152594
17	Phosphorylation	ELYSLNDYKPPISKA HHHHCCCCCCCCCHH	25.07	27273156
18	Sumoylation	LYSLNDYKPPISKAK HHHCCCCCCCCCHHH	47.37	-
18	Sumoylation	LYSLNDYKPPISKAK HHHCCCCCCCCCHHH	47.37	-
18	Ubiquitination	LYSLNDYKPPISKAK HHHCCCCCCCCCHHH	47.37	-
22	Phosphorylation	NDYKPPISKAKMTQI CCCCCCCCHHHHHHH	32.13	28152594
31	Ubiquitination	AKMTQITKAAIKAIK HHHHHHHHHHHHHHH	37.13	-
53	Ubiquitination	SVEKFIQKCKPEYKV HHHHHHHHCCCCCCC	39.13	-
81	Ubiquitination	RHQFGQEKDVFAPRF HHHHCCCCCCCCCCC	51.83	-
89	O-linked_Glycosylation	DVFAPRFSNNIISTF CCCCCCCCCCHHHHH	30.19	20068230
95	O-linked_Glycosylation	FSNNIISTFQNLYRC CCCCHHHHHHHHHCC	20.87	20068230
119	Ubiquitination	RVLNLWQKNNVFKSE HHHHHHHHCCCCHHH	38.62	21890473
119	Ubiquitination	RVLNLWQKNNVFKSE HHHHHHHHCCCCHHH	38.62	21890473
124	Ubiquitination	WQKNNVFKSEIIQPL HHHCCCCHHHHHHHH	42.78	-
185	Ubiquitination	WVSQITNTDTLAAVA HHHHCCCHHHHHHHH	23.01	-
197	Ubiquitination	AVAQILQSPQGQQLQ HHHHHHHCCCHHHHH	18.75	21890473
273	Phosphorylation	RFDFGEDSEHSEEPK CCCCCCCCCCCCCCC	32.67	23401153
276	Phosphorylation	FGEDSEHSEEPKKEI CCCCCCCCCCCCCCC	39.33	29255136
281	Ubiquitination	EHSEEPKKEIPASQL CCCCCCCCCCCHHHH	72.15	-
333	Phosphorylation	QKATPQDSQEGTFGS CCCCCCCCCCCCCCC	25.53	26074081
337	Phosphorylation	PQDSQEGTFGSEHSA CCCCCCCCCCCCCCC	24.87	26074081
340	Phosphorylation	SQEGTFGSEHSASPS CCCCCCCCCCCCCCC	28.13	26074081
343	Phosphorylation	GTFGSEHSASPSQGS CCCCCCCCCCCCCCC	26.79	26074081
345	Phosphorylation	FGSEHSASPSQGSSQ CCCCCCCCCCCCCCC	28.26	26074081
347	Phosphorylation	SEHSASPSQGSSQQH CCCCCCCCCCCCCCC	44.68	26074081
350	Phosphorylation	SASPSQGSSQQHFLE CCCCCCCCCCCCCCC	19.82	26074081
351	Phosphorylation	ASPSQGSSQQHFLEP CCCCCCCCCCCCCCC	40.46	26074081
402	Phosphorylation	AVRSRSRTHSRSRSR HHHHHCCCCCCCCCC	26.00	23828894
404	Phosphorylation	RSRSRTHSRSRSRSP HHHCCCCCCCCCCCC	31.19	23828894
406	Phosphorylation	RSRTHSRSRSRSPRK HCCCCCCCCCCCCCH	37.31	22468782
408	Phosphorylation	RTHSRSRSRSPRKRR CCCCCCCCCCCCHHH	38.05	20068231
410	Phosphorylation	HSRSRSRSPRKRRSR CCCCCCCCCCHHHHC	30.24	20068231
433	Phosphorylation	KHRKRSRSRSRERKR HHHHHHHHHHHHHHH	35.54	20068231
435	Phosphorylation	RKRSRSRSRERKRKS HHHHHHHHHHHHHHH	39.48	20068231
442	Phosphorylation	SRERKRKSSRSYSSE HHHHHHHHHHHHHHH	34.71	29449344
443	Phosphorylation	RERKRKSSRSYSSER HHHHHHHHHHHHHHH	28.42	22817900
445	Phosphorylation	RKRKSSRSYSSERRA HHHHHHHHHHHHHHH	31.17	30576142
446	Phosphorylation	KRKSSRSYSSERRAR HHHHHHHHHHHHHHH	18.33	29449344
447	Phosphorylation	RKSSRSYSSERRARE HHHHHHHHHHHHHHH	27.28	30576142
448	Phosphorylation	KSSRSYSSERRARER HHHHHHHHHHHHHHH	27.21	30576142
461	Acetylation	EREKERQKKGLPPIR HHHHHHHHCCCCCCC	55.84	7266349
533	Phosphorylation	FRALQKLSSGSYKIG HHHHHHHHCCCCCCC	39.42	21712546
534	Phosphorylation	RALQKLSSGSYKIGS HHHHHHHCCCCCCCH	42.04	21712546
536	Phosphorylation	LQKLSSGSYKIGSKV HHHHHCCCCCCCHHH	25.79	21712546
542	Ubiquitination	GSYKIGSKVIKIAWA CCCCCCHHHHHHHHH	42.65	-
604	Phosphorylation	TEWETVKSSEPVKET CCEEEECCCCCCCHH	35.28	23927012
605	Phosphorylation	EWETVKSSEPVKETV CEEEECCCCCCCHHH	39.95	23927012
605	O-linked_Glycosylation	EWETVKSSEPVKETV CEEEECCCCCCCHHH	39.95	20068230
609	Acetylation	VKSSEPVKETVQTTQ ECCCCCCCHHHCCCC	59.38	12654625
611	Phosphorylation	SSEPVKETVQTTQSP CCCCCCHHHCCCCCC	16.52	29255136
611	O-linked_Glycosylation	SSEPVKETVQTTQSP CCCCCCHHHCCCCCC	16.52	20068230
614	O-linked_Glycosylation	PVKETVQTTQSPTPV CCCHHHCCCCCCCCC	23.59	30059200
614	Phosphorylation	PVKETVQTTQSPTPV CCCHHHCCCCCCCCC	23.59	30266825
615	O-linked_Glycosylation	VKETVQTTQSPTPVE CCHHHCCCCCCCCCC	15.23	20068230
615	Phosphorylation	VKETVQTTQSPTPVE CCHHHCCCCCCCCCC	15.23	29255136
617	Phosphorylation	ETVQTTQSPTPVEKE HHHCCCCCCCCCCCC	28.75	19664994
619	Phosphorylation	VQTTQSPTPVEKETV HCCCCCCCCCCCCEE	45.25	29255136
686	O-linked_Glycosylation	LRASFNPSQPPPGFM CCCCCCCCCCCCCCC	57.32	20068230
779	Phosphorylation	HLIDHQISSGENTRS CCHHCCCCCCCCCCC	26.22	28348404
780	Phosphorylation	LIDHQISSGENTRSV CHHCCCCCCCCCCCC	52.32	28450419
784	Phosphorylation	QISSGENTRSVIPND CCCCCCCCCCCCCCC	21.73	28450419
807	Phosphorylation	GGQPPNVTSNSGILG CCCCCCCCCCCCCCC	28.91	22210691
810	Phosphorylation	PPNVTSNSGILGVQR CCCCCCCCCCCCCCC	27.29	22210691
821	O-linked_Glycosylation	GVQRPNVSSNSEILG CCCCCCCCCCCEEEC	30.37	20068230
822	O-linked_Glycosylation	VQRPNVSSNSEILGV CCCCCCCCCCEEECC	39.84	20068230
824	O-linked_Glycosylation	RPNVSSNSEILGVRP CCCCCCCCEEECCCC	28.14	20068230
861	O-linked_Glycosylation	ILGIQPPSVSNSSGL CCCCCCCCCCCCCCC	44.88	20068230
917	Asymmetric dimethylarginine	PLSIPNQRMPTMPML CCCCCCCCCCCCCCC	39.42	-
917	Methylation	PLSIPNQRMPTMPML CCCCCCCCCCCCCCC	39.42	24129315
927	Asymmetric dimethylarginine	TMPMLDIRPGLIPQA CCCCCCCCCCCCCCC	20.92	-
927	Methylation	TMPMLDIRPGLIPQA CCCCCCCCCCCCCCC	20.92	24129315
938	Methylation	IPQAPGPRFPLIQPG CCCCCCCCCCCCCCC	52.29	24129315
938	Asymmetric dimethylarginine	IPQAPGPRFPLIQPG CCCCCCCCCCCCCCC	52.29	-
967	Methylation	SALHPPPRGPFPPGD HCCCCCCCCCCCCCC	71.80	80702875
981	Methylation	DIFSQPERPFLAPGR CCCCCCCCCCCCCCC	33.67	80702883
988	Methylation	RPFLAPGRQSVDNVT CCCCCCCCCCCCCCC	25.25	80702891
990	Phosphorylation	FLAPGRQSVDNVTNP CCCCCCCCCCCCCCH	30.03	21815630
999	Acetylation	DNVTNPEKRIPLGND CCCCCHHHCCCCCCC	57.81	25953088
1026	Phosphorylation	PPIETRESISRPPPV CCCCCCCCCCCCCCC	23.67	28555341
1062	Methylation	DGRDHFGRPPVDIRE CCCCCCCCCCCCHHH	31.26	-
1073	Asymmetric dimethylarginine	DIRENLVRPGIDHLG CHHHHCCCCCCCCCC	27.22	-
1073	Methylation	DIRENLVRPGIDHLG CHHHHCCCCCCCCCC	27.22	54549787
1091	Ubiquitination	HFGFNPEKPWGHRGD CCCCCCCCCCCCCCC	47.53	-
1120	Methylation	PKGLHEERGRFRSGN CCCHHCCCCCCCCCC	38.31	18601187
1122	Methylation	GLHEERGRFRSGNYR CHHCCCCCCCCCCCC	29.99	-
1124	Methylation	HEERGRFRSGNYRFD HCCCCCCCCCCCCCC	42.15	-
1129	Methylation	RFRSGNYRFDPRSGP CCCCCCCCCCCCCCC	33.23	-
1133	Methylation	GNYRFDPRSGPWNRG CCCCCCCCCCCCCCC	56.97	-
1139	Methylation	PRSGPWNRGFGQEVH CCCCCCCCCCCCHHC	37.64	30760801
1198	Phosphorylation	PHARVFDYFEGATSQ CCCCHHCCCCCCCCC	7.65	-
1237	Phosphorylation	VQNDPELYEKLTSSN CCCCHHHHHHHHCCC	14.89	28796482
1241	Phosphorylation	PELYEKLTSSNEINK HHHHHHHHCCCCCCH	40.97	22468782
1251	Phosphorylation	NEINKEKSDTVADIE CCCCHHHCCCCCCCC	39.34	22468782

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of SCAF8_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SCAF8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SCAF8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of SCAF8_HUMAN !!

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SCAF8_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617 AND THR-619, ANDMASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617, AND MASSSPECTROMETRY.	18669648 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-615 AND SER-617, ANDMASS SPECTROMETRY.	17525332 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617, AND MASSSPECTROMETRY.	17081983 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617, AND MASSSPECTROMETRY.	15302935 [Abstract]