dbPTM

S4A4_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	S4A4_RAT
UniProt AC	Q9JI66
Protein Name	Electrogenic sodium bicarbonate cotransporter 1
Gene Name	Slc4a4
Organism	Rattus norvegicus (Rat).
Sequence Length	1079
Subcellular Localization	Basolateral cell membrane Multi-pass membrane protein .
Protein Description	Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.; Isoform 2: May have a higher activity than isoform 1..
Protein Sequence	MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKAGHKEKKEKERISENYSDKSDVENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIADHQIETGLLKPDLKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFSNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDHSFDYLEFRLWIGLWSAFMCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSDFRVGYNTHFSCACLPPDPVNLSVSNDTTLAPEDLPTVSSTDMYHNATFDWAYLSKKECVKFGGKLVGNNCDFVPDITLMSFILFLGTYTSSMAMKKFKTSRYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPHRGWFVPPFGGNPWWVCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVVCSFMALPWYVAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFLYMGVASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTSLQVLCLALLWILKSTVAAIIFPVMILALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDSDCPYSEKVPSIKIPMDITEQQPFLSDNKPLDRERSSTFLERHTSC

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2 (in isoform 2)	Phosphorylation	-	75.71	23800682
3 (in isoform 2)	Phosphorylation	-	62.10	22673903
12	Phosphorylation	AVLDRGASFLKHVCD HHHHHHHHHHHHHCC	33.66	29779826
21 (in isoform 2)	Phosphorylation	-	54.84	22673903
22 (in isoform 2)	Phosphorylation	-	43.77	22673903
23 (in isoform 2)	Phosphorylation	-	13.94	22673903
30	Phosphorylation	VEGHHTIYIGVHVPK HCCCEEEEEEEECCH	7.87	-
61	Phosphorylation	KKEKERISENYSDKS HHHHHHHCCCCCCHH	26.47	27097102
64	Phosphorylation	KERISENYSDKSDVE HHHHCCCCCCHHHHC	17.75	27097102
65	Phosphorylation	ERISENYSDKSDVEN HHHCCCCCCHHHHCC	50.58	27097102
67	Acetylation	ISENYSDKSDVENAD HCCCCCCHHHHCCCC	42.49	22902405
68	Phosphorylation	SENYSDKSDVENADE CCCCCCHHHHCCCCC	52.45	27097102
76	Phosphorylation	DVENADESSSSILKP HHCCCCCCCCHHHHH	35.75	27097102
77	Phosphorylation	VENADESSSSILKPL HCCCCCCCCHHHHHH	26.51	27097102
78	Phosphorylation	ENADESSSSILKPLI CCCCCCCCHHHHHHH	30.58	27097102
79	Phosphorylation	NADESSSSILKPLIS CCCCCCCHHHHHHHC	33.61	27097102
82	Acetylation	ESSSSILKPLISPAA CCCCHHHHHHHCCHH	35.79	22902405
86	Phosphorylation	SILKPLISPAAERIR HHHHHHHCCHHHHHH	19.03	27097102
137	Acetylation	RWIKFEEKVEQGGER HHHHHHHHHHHCCCC	43.82	22902405
174	Methylation	KGSIMLDREASSLPQ HCCEEECCCCCCHHH	37.10	26494671
203	Ubiquitination	LKPDLKDKVTYTLLR CCCCHHHHHHHHHHH	34.58	-
203 (in isoform 3)	Ubiquitination	-	34.58	-
219	Phosphorylation	HRHQTKKSNLRSLAD HCHHCCHHCHHHHHH	42.53	23984901
223	Phosphorylation	TKKSNLRSLADIGKT CCHHCHHHHHHHCCH	30.95	29779826
229	Ubiquitination	RSLADIGKTVSSASR HHHHHHCCHHHHHHH	46.19	-
229 (in isoform 3)	Ubiquitination	-	46.19	-
230	Phosphorylation	SLADIGKTVSSASRM HHHHHCCHHHHHHHH	22.02	25575281
232	Phosphorylation	ADIGKTVSSASRMFS HHHCCHHHHHHHHHC	25.97	29779826
233	Phosphorylation	DIGKTVSSASRMFSN HHCCHHHHHHHHHCC	26.40	28689409
235	Phosphorylation	GKTVSSASRMFSNPD CCHHHHHHHHHCCCC	26.65	28689409
239	Phosphorylation	SSASRMFSNPDNGSP HHHHHHHCCCCCCCC	37.80	27097102
245	Phosphorylation	FSNPDNGSPAMTHRN HCCCCCCCCCCCCCC	18.75	28689409
249	Phosphorylation	DNGSPAMTHRNLTSS CCCCCCCCCCCCCCC	21.57	27097102
254	Phosphorylation	AMTHRNLTSSSLNDI CCCCCCCCCCCCCCC	30.06	29779826
255	Phosphorylation	MTHRNLTSSSLNDIS CCCCCCCCCCCCCCC	22.54	27097102
256	Phosphorylation	THRNLTSSSLNDISD CCCCCCCCCCCCCCC	33.34	27097102
257	Phosphorylation	HRNLTSSSLNDISDK CCCCCCCCCCCCCCC	30.84	23712012
262	Phosphorylation	SSSLNDISDKPEKDQ CCCCCCCCCCCCHHH	42.31	27097102
264	Acetylation	SLNDISDKPEKDQLK CCCCCCCCCCHHHHH	48.57	22902405
448	Acetylation	RFCGGLIKDIKRKAP CCCCHHHHHHHHHCC	59.45	22902405
641	N-linked_Glycosylation	PVNLSVSNDTTLAPE CCEEEECCCCCCCCC	48.79	12604466
661	N-linked_Glycosylation	SSTDMYHNATFDWAY CCCCCCCCCCCCEEE	24.16	12604466
676	Acetylation	LSKKECVKFGGKLVG CCHHHHHHCCCEECC	50.37	22902405
705	Phosphorylation	ILFLGTYTSSMAMKK HHHHHHHCCHHHHHH	17.73	-
760	Acetylation	LIVPSEFKPTSPHRG EECCCCCCCCCCCCC	43.25	22902405
1026	Phosphorylation	KKKKKKGSLDSDNDD HHHHHCCCCCCCCCC	37.57	29779826
1026 (in isoform 3)	Phosphorylation	-	37.57	27097102
1029	Phosphorylation	KKKGSLDSDNDDSDC HHCCCCCCCCCCCCC	43.54	29779826
1029 (in isoform 3)	Phosphorylation	-	43.54	27097102
1034	Phosphorylation	LDSDNDDSDCPYSEK CCCCCCCCCCCCCCC	43.93	29779826
1038	Phosphorylation	NDDSDCPYSEKVPSI CCCCCCCCCCCCCCC	35.47	28551015
1039	Phosphorylation	DDSDCPYSEKVPSIK CCCCCCCCCCCCCCC	18.98	28551015
1044	Phosphorylation	PYSEKVPSIKIPMDI CCCCCCCCCCCCCCC	39.23	27097102
1052	Phosphorylation	IKIPMDITEQQPFLS CCCCCCCCCCCCCCC	24.39	28432305
1055 (in isoform 3)	Phosphorylation	-	25.20	28551015
1060 (in isoform 3)	Phosphorylation	-	70.13	27097102
1062	Acetylation	QPFLSDNKPLDRERS CCCCCCCCCCCHHHH	52.53	22902405
1064 (in isoform 3)	Phosphorylation	-	11.78	27097102
1067 (in isoform 3)	Ubiquitination	-	57.49	-
1069	Phosphorylation	KPLDRERSSTFLERH CCCCHHHHHHHHHHH	29.04	29779826
1070	Phosphorylation	PLDRERSSTFLERHT CCCHHHHHHHHHHHC	29.42	27097102
1071	Phosphorylation	LDRERSSTFLERHTS CCHHHHHHHHHHHCC	33.22	27097102
1078 (in isoform 3)	Phosphorylation	-	16.23	27097102
1083 (in isoform 3)	Phosphorylation	-		28551015

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of S4A4_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
1026	S	Phosphorylation		22673903
Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of S4A4_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of S4A4_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of S4A4_RAT

Related Literatures of Post-Translational Modification

N-linked Glycosylation
Reference	PubMed
"Role of glycosylation in the renal electrogenic Na+-HCO3-cotransporter (NBCe1)."; Choi I., Hu L., Rojas J.D., Schmitt B.M., Boron W.F.; Am. J. Physiol. 284:F1199-F1206(2003). Cited for: MUTAGENESIS OF ASN-243; ASN-252; ASN-541; ASN-636; ASN-641 ANDASN-661, AND GLYCOSYLATION AT ASN-641 AND ASN-661.	12604466 [Abstract]

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