dbPTM

S12A6_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	S12A6_HUMAN
UniProt AC	Q9UHW9
Protein Name	Solute carrier family 12 member 6
Gene Name	SLC12A6
Organism	Homo sapiens (Human).
Sequence Length	1150
Subcellular Localization	Basolateral cell membrane Multi-pass membrane protein.
Protein Description	Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells..
Protein Sequence	MHPPETTTKMASVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSEMSGATTSLATVALDPPSDRTSHPQDVIEDLSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5 (in isoform 2)	Phosphorylation	-	64.34	-
5 (in isoform 6)	Phosphorylation	-	64.34	-
12	Phosphorylation	ETTTKMASVRFMVTP CCCCCCEEEEEEECC	15.44	26074081
18	Phosphorylation	ASVRFMVTPTKIDDI EEEEEEECCCCHHCC	17.29	22199227
19 (in isoform 2)	Phosphorylation	-	21.93	26657352
19 (in isoform 6)	Phosphorylation	-	21.93	26657352
20	Phosphorylation	VRFMVTPTKIDDIPG EEEEECCCCHHCCCC	31.13	26074081
21 (in isoform 2)	Phosphorylation	-	45.39	26657352
21 (in isoform 6)	Phosphorylation	-	45.39	26657352
25 (in isoform 2)	Phosphorylation	-	2.30	26657352
25 (in isoform 6)	Phosphorylation	-	2.30	26657352
29	Phosphorylation	IDDIPGLSDTSPDLS HHCCCCCCCCCCCCC	44.77	30266825
30 (in isoform 2)	Ubiquitination	-	57.13	21890473
31	Phosphorylation	DIPGLSDTSPDLSSR CCCCCCCCCCCCCCC	38.49	30266825
32	Phosphorylation	IPGLSDTSPDLSSRS CCCCCCCCCCCCCCC	22.77	23401153
36	Phosphorylation	SDTSPDLSSRSSSRV CCCCCCCCCCCCCCC	30.96	29255136
36 (in isoform 2)	Phosphorylation	-	30.96	28450419
37	Phosphorylation	DTSPDLSSRSSSRVR CCCCCCCCCCCCCCC	43.40	29255136
39	Phosphorylation	SPDLSSRSSSRVRFS CCCCCCCCCCCCCCC	34.31	29116813
40	Phosphorylation	PDLSSRSSSRVRFSS CCCCCCCCCCCCCCC	22.51	29116813
41	Phosphorylation	DLSSRSSSRVRFSSR CCCCCCCCCCCCCCC	35.72	29116813
42 (in isoform 2)	Phosphorylation	-	26.11	30108239
45 (in isoform 2)	Phosphorylation	-	7.71	25159151
46	Phosphorylation	SSSRVRFSSRESVPE CCCCCCCCCCCCCCC	20.23	28450419
47	Phosphorylation	SSRVRFSSRESVPET CCCCCCCCCCCCCCC	37.04	20363803
47 (in isoform 2)	Phosphorylation	-	37.04	30108239
50	Phosphorylation	VRFSSRESVPETSRS CCCCCCCCCCCCCCC	41.69	20363803
51 (in isoform 2)	Phosphorylation	-	4.21	28450419
54	Phosphorylation	SRESVPETSRSEPMS CCCCCCCCCCCCCHH	24.30	27486199
61	Phosphorylation	TSRSEPMSEMSGATT CCCCCCHHHCCCCCC	40.64	18669648
64	Phosphorylation	SEPMSEMSGATTSLA CCCHHHCCCCCCCEE	22.64	23532336
67	Phosphorylation	MSEMSGATTSLATVA HHHCCCCCCCEEEEE	22.05	24043423
68	Phosphorylation	SEMSGATTSLATVAL HHCCCCCCCEEEEEC	22.62	24043423
69	Phosphorylation	EMSGATTSLATVALD HCCCCCCCEEEEECC	16.20	18669648
72	Phosphorylation	GATTSLATVALDPPS CCCCCEEEEECCCCC	16.17	25954137
79	Phosphorylation	TVALDPPSDRTSHPQ EEECCCCCCCCCCCH	45.15	23322592
80 (in isoform 2)	Ubiquitination	-	64.75	-
93	Phosphorylation	QDVIEDLSQNSITGE HHHHHHHHHCCCCCC	39.37	25921289
96	Phosphorylation	IEDLSQNSITGEHSQ HHHHHHCCCCCCHHH	17.56	23401153
98	Phosphorylation	DLSQNSITGEHSQLL HHHHCCCCCCHHHHC	36.02	30108239
102	Phosphorylation	NSITGEHSQLLDDGH CCCCCCHHHHCCHHC	20.44	30108239
116	Phosphorylation	HKKARNAYLNNSNYE CHHHHHHHHCCCCCC	17.32	21945579
120	Phosphorylation	RNAYLNNSNYEEGDE HHHHHCCCCCCCCHH	39.45	21945579
122	Phosphorylation	AYLNNSNYEEGDEYF HHHCCCCCCCCHHHH	18.38	21945579
125 (in isoform 2)	Ubiquitination	-	22.55	21890473
128	Phosphorylation	NYEEGDEYFDKNLAL CCCCCHHHHHHHHHH	23.09	22199227
131	Ubiquitination	EGDEYFDKNLALFEE CCHHHHHHHHHHHHH	43.92	-
145	Ubiquitination	EEMDTRPKVSSLLNR HHHCCCHHHHHHHHH	52.00	-
147	Phosphorylation	MDTRPKVSSLLNRMA HCCCHHHHHHHHHHH	22.76	29978859
148	Phosphorylation	DTRPKVSSLLNRMAN CCCHHHHHHHHHHHH	39.60	23401153
156	Phosphorylation	LLNRMANYTNLTQGA HHHHHHHHCCCCHHH	6.35	21945579
157	Phosphorylation	LNRMANYTNLTQGAK HHHHHHHCCCCHHHH	24.45	21945579
160	Phosphorylation	MANYTNLTQGAKEHE HHHHCCCCHHHHHHH	27.33	28851738
164	Ubiquitination	TNLTQGAKEHEEAEN CCCCHHHHHHHHHHC	67.44	-
176	Ubiquitination	AENITEGKKKPTKTP HHCCCCCCCCCCCCC	51.93	2190698
176 (in isoform 1)	Ubiquitination	-	51.93	21890473
182	Phosphorylation	GKKKPTKTPQMGTFM CCCCCCCCCCHHHHH	22.64	24043423
187	Phosphorylation	TKTPQMGTFMGVYLP CCCCCHHHHHHHHHH	12.71	24043423
192	Phosphorylation	MGTFMGVYLPCLQNI HHHHHHHHHHHHHHH	10.13	24043423
321	Phosphorylation	MLNNMRVYGTAFLVL HHHCCHHHHHHHHHH	9.82	-
323	Phosphorylation	NNMRVYGTAFLVLMV HCCHHHHHHHHHHHH	9.13	-
379	N-linked_Glycosylation	FPVCMLGNRTLSSRH CCEEEECCCCCCCCC	29.78	UniProtKB CARBOHYD
398	N-linked_Glycosylation	SKTKEINNMTVPSKL CCCCCCCCCCCCHHH	32.91	UniProtKB CARBOHYD
411	N-linked_Glycosylation	KLWGFFCNSSQFFNA HHHHHHCCHHHHHCC	39.27	UniProtKB CARBOHYD
428	N-linked_Glycosylation	DEYFVHNNVTSIQGI CCEEEECCCCCCCCC	24.46	UniProtKB CARBOHYD
460	Acetylation	PKGEIIEKPSAKSSD CCCCCCCCCCCCCCC	33.59	11922915
460	Ubiquitination	PKGEIIEKPSAKSSD CCCCCCCCCCCCCCC	33.59	-
464	Acetylation	IIEKPSAKSSDVLGS CCCCCCCCCCCCCCC	55.63	11922927
465	Phosphorylation	IEKPSAKSSDVLGSL CCCCCCCCCCCCCCC	31.33	22468782
541	Ubiquitination	NVVLFGACIEGVVLR CCHHHHHHEECCCCH	2.73	21890473
549	Ubiquitination	IEGVVLRDKFGDAVK EECCCCHHCCCCHHC	46.61	21890473
549 (in isoform 2)	Ubiquitination	-	46.61	21890473
585	Ubiquitination	FSTCGAGLQSLTGAP HHCCCHHHHHHHCHH	2.87	21890473
591	Ubiquitination	GLQSLTGAPRLLQAI HHHHHHCHHHHHHHH	4.84	21890473
600	Acetylation	RLLQAIAKDNIIPFL HHHHHHHHCCCHHHH	45.23	23954790
600	Ubiquitination	RLLQAIAKDNIIPFL HHHHHHHHCCCHHHH	45.23	-
600 (in isoform 1)	Ubiquitination	-	45.23	21890473
673 (in isoform 2)	Ubiquitination	-	21.11	21890473
724	Ubiquitination	IEYQGAEKEWGDGIR HHHCCCCCCCCCCHH	59.23	-
724 (in isoform 1)	Ubiquitination	-	59.23	21890473
734	Phosphorylation	GDGIRGLSLSAARFA CCCHHHHHHHHHHHH	24.09	30108239
736	Phosphorylation	GIRGLSLSAARFALL CHHHHHHHHHHHHHH	19.26	30108239
772	Ubiquitination	LDEDLHVKHPRLLTF CCCCCCCCCHHHHHH	36.82	-
778	Phosphorylation	VKHPRLLTFASQLKA CCCHHHHHHHHHHHC	23.03	-
781	Phosphorylation	PRLLTFASQLKAGKG HHHHHHHHHHHCCCC	31.67	-
784	Ubiquitination	LTFASQLKAGKGLTI HHHHHHHHCCCCCEE	47.67	-
940	Phosphorylation	HKVWRKCSIRIFTVA CHHHHHCCEEEEEEE	20.24	25278378
945	Phosphorylation	KCSIRIFTVAQLEDN HCCEEEEEEEEECCC	16.74	25278378
965	Phosphorylation	KDLATFLYHLRIEAE HHHHHHHHHHHHCEE	8.46	24114839
981	Phosphorylation	EVVEMHDSDISAYTY EEEECCCCCCHHHHH	23.36	18669648
991	Phosphorylation	SAYTYERTLMMEQRS HHHHHHHHHHHHHHH	13.68	23401153
1007	Phosphorylation	MLRHMRLSKTERDRE HHHHHHCCCCHHHHH	27.45	-
1019	Ubiquitination	DREAQLVKDRNSMLR HHHHHHHHCCHHCCC	60.61	-
1023	Phosphorylation	QLVKDRNSMLRLTSI HHHHCCHHCCCEEEC	21.79	23401153
1028	Phosphorylation	RNSMLRLTSIGSDED CHHCCCEEECCCCCC	16.32	22167270
1029	Phosphorylation	NSMLRLTSIGSDEDE HHCCCEEECCCCCCC	29.55	22167270
1032	Phosphorylation	LRLTSIGSDEDEETE CCEEECCCCCCCCCC	35.84	10187864
1038	Phosphorylation	GSDEDEETETYQEKV CCCCCCCCCHHHHHH	31.42	22167270
1040	Phosphorylation	DEDEETETYQEKVHM CCCCCCCHHHHHHHC	37.61	23403867
1048	Phosphorylation	YQEKVHMTWTKDKYM HHHHHHCHHCHHHHH	18.33	23401153
1050	Phosphorylation	EKVHMTWTKDKYMAS HHHHCHHCHHHHHHH	21.87	23403867
1063	Ubiquitination	ASRGQKAKSMEGFQD HHCCHHCHHCHHHHH	58.32	-
1064	Phosphorylation	SRGQKAKSMEGFQDL HCCHHCHHCHHHHHH	27.25	23898821
1086	Phosphorylation	SNVRRMHTAVKLNEV HHHHHHHHHHHHCEE	24.65	-
1097	Ubiquitination	LNEVIVNKSHEAKLV HCEEEECCCCCEEEE	42.15	-
1121	Phosphorylation	NPEGDENYMEFLEVL CCCCCCCHHHHHHHH	8.93	22817900
1129	Phosphorylation	MEFLEVLTEGLERVL HHHHHHHHHCCCEEE	32.93	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
96	S	Phosphorylation	Kinase	STK39	Q9UEW8	GPS
96	S	Phosphorylation	Kinase	WNK3	Q9BYP7	PSP
160	T	Phosphorylation	Kinase	STK39	Q9UEW8	GPS
991	T	Phosphorylation	Kinase	WNK1	Q9H4A3	PSP
991	T	Phosphorylation	Kinase	WNK3	Q9BYP7	PSP
1048	T	Phosphorylation	Kinase	STK39	Q9UEW8	GPS
1048	T	Phosphorylation	Kinase	WNK1	Q9H4A3	PSP
1048	T	Phosphorylation	Kinase	WNK3	Q9BYP7	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of S12A6_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of S12A6_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of S12A6_HUMAN !!

Drug and Disease Associations

Kegg Disease
H00816	Agenesis of the corpus callosum with peripheral neuropathy (ACCPN)
OMIM Disease
218000	Agenesis of the corpus callosum, with peripheral neuropathy (ACCPN)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
DB00761	Potassium Chloride

Regulatory Network of S12A6_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, AND MASSSPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1029 AND SER-1032, ANDMASS SPECTROMETRY.	18669648 [Abstract]