S12A5_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: S12A5_RAT
• UniProt AC: Q63633
• Protein Name: Solute carrier family 12 member 5
• Gene Name: Slc12a5
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1139
• Subcellular Localization: Membrane; Multi-pass membrane protein.
• Protein Description: Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. [PubMed: 9930699 Involved in the regulation of dendritic spine formation and maturation]
• Protein Sequence: MSRRFTVTSLPPAASAASADPESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSAAQKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8	Phosphorylation	MSRRFTVTSLPPAAS CCCCEEECCCCCCHH	22.84	28432305
9	Phosphorylation	SRRFTVTSLPPAASA CCCEEECCCCCCHHH	34.24	28432305
25 (in isoform 2)	Phosphorylation	-	35.39	-
26 (in isoform 2)	Phosphorylation	-	26.05	27115346
48	Phosphorylation	GDGNPKESSPFINST CCCCCCCCCCCCCCC	49.81	25403869
49	Phosphorylation	DGNPKESSPFINSTD CCCCCCCCCCCCCCC	25.56	30240740
54	Phosphorylation	ESSPFINSTDTEKGR CCCCCCCCCCCCCCC	23.89	25403869
55	Phosphorylation	SSPFINSTDTEKGRE CCCCCCCCCCCCCCE	42.31	22673903
57	Phosphorylation	PFINSTDTEKGREYD CCCCCCCCCCCCEEC	39.42	25403869
59	Ubiquitination	INSTDTEKGREYDGR CCCCCCCCCCEECCC	66.42	-
63	Phosphorylation	DTEKGREYDGRNMAL CCCCCCEECCCCCHH	23.49	-
442	N-linked_Glycosylation	TGIMAGSNRSGDLRD CEECCCCCCCCCHHH	41.02	-
691	Ubiquitination	EEGPPHTKNWRPQLL CCCCCCCCCCCCEEE	52.02	-
833	N-linked_Glycosylation	LALLVTKNVSMFPGN HHEEEECCCCCCCCC	23.02	-
895	Ubiquitination	DDNSIQMKKDLTTFL CCCCEECHHHHHHHH	27.10	-
896	Ubiquitination	DNSIQMKKDLTTFLY CCCEECHHHHHHHHH	53.26	-
906 (in isoform 2)	Phosphorylation	-	15.76	-
926	Phosphorylation	SDISAYTYEKTLVME CCCCEEEECCHHHHH	11.62	20600929
929	Phosphorylation	SAYTYEKTLVMEQRS CEEEECCHHHHHHHH	16.79	-
934 (in isoform 2)	Phosphorylation	-	32.03	-
937 (in isoform 2)	Phosphorylation	-	47.42	-
940 (in isoform 2)	Phosphorylation	-	47.40	-
940	Ubiquitination	EQRSQILKQMHLTKN HHHHHHHHHHCCCCC	47.40	-
957	Phosphorylation	EREIQSITDESRGSI HHHHHHCCCCCCCCC	38.67	-
960	Phosphorylation	IQSITDESRGSIRRK HHHCCCCCCCCCCCC	44.76	25403869
963	Phosphorylation	ITDESRGSIRRKNPA CCCCCCCCCCCCCCC	16.29	25403869
1007 (in isoform 2)	Phosphorylation	-	48.01	-
1007	Phosphorylation	QSAPSCPSSSPSPGE CCCCCCCCCCCCCCC	48.01	30322021
1008	Phosphorylation	SAPSCPSSSPSPGEE CCCCCCCCCCCCCCC	31.26	30322021
1009	Phosphorylation	APSCPSSSPSPGEEP CCCCCCCCCCCCCCC	33.46	30322021
1011	Phosphorylation	SCPSSSPSPGEEPEG CCCCCCCCCCCCCCC	47.70	30322021
1022 (in isoform 2)	Phosphorylation	-	68.65	-
1025 (in isoform 2)	Phosphorylation	-	71.20	-
1026 (in isoform 2)	Phosphorylation	-	40.10	-
1030	Phosphorylation	DPEKVHLTWTKDKSA CHHHEEEEEECCHHH	19.06	30411139
1045	Phosphorylation	AQKNKGPSPVSSEGI HHHCCCCCCCCCCHH	46.75	30240740
1048	Phosphorylation	NKGPSPVSSEGIKDF CCCCCCCCCCHHHHH	26.51	25403869
1049	Phosphorylation	KGPSPVSSEGIKDFF CCCCCCCCCHHHHHH	39.82	25403869
1053	Ubiquitination	PVSSEGIKDFFSMKP CCCCCHHHHHHHCCH	60.38	-
1086	Ubiquitination	LNEVIVNKSRDAKLV HCEEEEECCCCCCEE	35.65	-
1110	Phosphorylation	NRNGDENYMEFLEVL CCCCCCCHHHHHHHH	8.93	20600929

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
940	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
963	S	Phosphorylation	Kinase	PRKCA	P17252	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of S12A5_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of S12A5_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of S12A5_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.