S12A5_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: S12A5_HUMAN
• UniProt AC: Q9H2X9
• Protein Name: Solute carrier family 12 member 5
• Gene Name: SLC12A5
• Organism: Homo sapiens (Human).
• Sequence Length: 1139
• Subcellular Localization: Membrane; Multi-pass membrane protein.
• Protein Description: Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (By similarity). Involved in the regulation of dendritic spine formation and maturation. [PubMed: 24668262]
• Protein Sequence: MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSRRFTVTS ------CCCCEEECC	43.39	24043423
6	Phosphorylation	--MSRRFTVTSLPPA --CCCCEEECCCCCC	22.51	30266825
8	Phosphorylation	MSRRFTVTSLPPAGP CCCCEEECCCCCCCC	22.84	30266825
9	Phosphorylation	SRRFTVTSLPPAGPA CCCEEECCCCCCCCC	34.24	30266825
18	Phosphorylation	PPAGPARSPDPESRR CCCCCCCCCCHHHHC	35.44	30266825
23	Phosphorylation	ARSPDPESRRHSVAD CCCCCHHHHCCCCCC	39.75	30266825
27	Phosphorylation	DPESRRHSVADPRHL CHHHHCCCCCCCCCC	19.72	29691806
49	Phosphorylation	DGNPKESSPFINSTD CCCCCCCCCCCCCCC	25.56	24076635
54	Phosphorylation	ESSPFINSTDTEKGK CCCCCCCCCCCCCCC	23.89	-
57	Phosphorylation	PFINSTDTEKGKEYD CCCCCCCCCCCCCCC	39.42	-
77	Phosphorylation	LFEEEMDTSPMVSSL HHHHHHCCCHHHHHH	33.16	-
78	Phosphorylation	FEEEMDTSPMVSSLL HHHHHCCCHHHHHHH	13.51	-
82	Phosphorylation	MDTSPMVSSLLSGLA HCCCHHHHHHHHHHH	14.71	-
92	Phosphorylation	LSGLANYTNLPQGSR HHHHHHCCCCCCCCH	30.04	-
335	Phosphorylation	LAWEGNETVTTRLWG ECCCCCCCHHHHHHH	27.44	27251275
337	Phosphorylation	WEGNETVTTRLWGLF CCCCCCHHHHHHHHH	17.39	27251275
338	Phosphorylation	EGNETVTTRLWGLFC CCCCCHHHHHHHHHH	21.30	27251275
442	N-linked_Glycosylation	TGIMAGSNRSGDLRD CEECCCCCCCCCHHH	41.02	UniProtKB CARBOHYD
444	Phosphorylation	IMAGSNRSGDLRDAQ ECCCCCCCCCHHHHH	40.69	-
501	Phosphorylation	NGNLVVGTLAWPSPW CCCEEEEECCCCCCE	11.16	-
537	Phosphorylation	PRLLQAISRDGIVPF HHHHHHHHCCCCEEE	27.60	29083192
617	Phosphorylation	WRPRFRYYHWTLSFL CCCCCHHHHHHHHHH	6.14	-
622	Phosphorylation	RYYHWTLSFLGMSLC HHHHHHHHHHHHHHH	15.85	-
668	Ubiquitination	EKEWGDGIRGLSLSA CCHHCCCHHHHHHHH	3.62	22817900
672	Phosphorylation	GDGIRGLSLSAARYA CCCHHHHHHHHHHHH	24.09	30108239
674	Phosphorylation	GIRGLSLSAARYALL CHHHHHHHHHHHHHH	19.26	30108239
691	Ubiquitination	EEGPPHTKNWRPQLL CCCCCCCCCCCCEEE	52.02	22817900
702	Ubiquitination	PQLLVLVRVDQDQNV CEEEEEEEECCCCCC	23.33	30230243
725	Ubiquitination	TSQLKAGKGLTIVGS HHHHHCCCCEEEEEE	56.45	30230243
751	Phosphorylation	QAQRAEESIRRLMEA HHHHHHHHHHHHHHH	16.95	26437602
770	Phosphorylation	GFCQVVISSNLRDGV CEEEEEECCCCCCHH	11.10	30576142
821	Phosphorylation	FIELVRETTAGHLAL HHHHHHHCCHHHHHE	16.04	-
831	Phosphorylation	GHLALLVTKNVSMFP HHHHEEEECCCCCCC	19.50	-
833	N-linked_Glycosylation	LALLVTKNVSMFPGN HHEEEECCCCCCCCC	23.02	UniProtKB CARBOHYD
872	Ubiquitination	PFLLRHHKVWRKCKM HHHHHCHHHHHHCCC	37.05	30230243
895	Ubiquitination	DDNSIQMKKDLTTFL CCCCEECHHHHHHHH	27.10	30230243
929	Phosphorylation	SAYTYEKTLVMEQRS CEEEECCHHHHHHHH	16.79	19665974
936	Phosphorylation	TLVMEQRSQILKQMH HHHHHHHHHHHHHHC	22.67	23401153
945	Phosphorylation	ILKQMHLTKNERERE HHHHHCCCCCHHHHH	19.69	27762562
960	Phosphorylation	IQSITDESRGSIRRK HHHCCCCCCCCCCCC	44.76	30108239
963	Phosphorylation	ITDESRGSIRRKNPA CCCCCCCCCCCCCCC	16.29	30108239
1007	Phosphorylation	QSAPSCPSSSPSPGE CCCCCCCCCCCCCCC	48.01	32142685
1030	Phosphorylation	DPEKVHLTWTKDKSV CHHHEEEEEECCHHH	19.06	19665974
1032	Phosphorylation	EKVHLTWTKDKSVAE HHEEEEEECCHHHHH	24.91	-
1045	Phosphorylation	AEKNKGPSPVSSEGI HHHCCCCCCCCCCHH	46.75	24076635
1048	Phosphorylation	NKGPSPVSSEGIKDF CCCCCCCCCCHHHHH	26.51	25307156
1049	Phosphorylation	KGPSPVSSEGIKDFF CCCCCCCCCHHHHHH	39.82	-
1057	Phosphorylation	EGIKDFFSMKPEWEN CHHHHHHHCCHHHHC	26.37	24719451
1110	Phosphorylation	NRNGDENYMEFLEVL CCCCCCCHHHHHHHH	8.93	10942735

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
6	T	Phosphorylation	Kinase	STK39	Q9UEW8	GPS
1032	T	Phosphorylation	Kinase	STK39	Q9UEW8	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of S12A5_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of S12A5_HUMAN !!

Protein-Protein Interaction

Oops, there are no PPI records of S12A5_HUMAN !!

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00887: Bumetanide
• DB00761: Potassium Chloride