dbPTM

RPGF2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RPGF2_MOUSE
UniProt AC	Q8CHG7
Protein Name	Rap guanine nucleotide exchange factor 2
Gene Name	Rapgef2
Organism	Mus musculus (Mouse).
Sequence Length	1496
Subcellular Localization	Cytoplasm. Cytoplasm, perinuclear region. Cell membrane. Late endosome. Cell junction. Associated with the synaptic plasma membrane. Localized diffusely in the cytoplasm before neuronal growth factor (NGF) stimulation. Recruited to late endosomes aft
Protein Description	Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions..
Protein Sequence	MKPLAAPANHGVLGQQEKQSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSCSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPAALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPEQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLSGQNLQPPVIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSANATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATSTLPKNPGDKKPVKSETSPVAPRAGPQQKVQPQQPLAQPQPPHKVSQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRKQAEDTISNASSQLSSPPTSPQSSPRKGYALALSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVESNLGVGRMERRTLMEPDQYSLGSYAPVSESRGLYAAATVISSPSTEELSHDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDAASIWASGGHMDQMMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSAEAESSSMVPVTTEEAKPVPMPAHIAVTPSTTKGLIARKEGRYREPPPTPPGYVGIPIADFPEGPCHPARKPPDYNVALQRSRMVARPTEAPAPGQTPPAAAASRPGSKPQWHKPSDADPRLAPFQPQGFAGAEEDEDEQVSAV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
60	Phosphorylation	DSGSSSLSDIYQATE CCCCCCHHHHHHHHH	24.74	17203969
62	Phosphorylation	GSSSLSDIYQATESE CCCCHHHHHHHHHHC	2.17	17203969
307	Phosphorylation	LTYRTFLSSPMEVGK HHHHHHHCCHHHHHH	28.36	17203969
308	Phosphorylation	TYRTFLSSPMEVGKK HHHHHHCCHHHHHHH	30.34	17203969
418	Phosphorylation	IFVDSVDSCSKATEA EEEECHHHCCHHHHH	20.38	17203969
420	Phosphorylation	VDSVDSCSKATEAGL EECHHHCCHHHHHHH	29.31	17203969
460	Phosphorylation	LRNNTHLSITVKTNL HHCCCCEEEEEECCH	14.49	29472430
462	Phosphorylation	NNTHLSITVKTNLFV CCCCEEEEEECCHHH	16.75	29472430
478	Phosphorylation	KELLTRLSEEKRNGA HHHHHHHCHHHHCCC	40.18	29899451
498	Phosphorylation	IGDIKKASRYSIPDL CCCHHHHHCCCCCCC	40.40	26239621
500	Phosphorylation	DIKKASRYSIPDLAV CHHHHHCCCCCCCCC	14.41	26239621
501	Phosphorylation	IKKASRYSIPDLAVD HHHHHCCCCCCCCCC	26.91	26239621
558	Phosphorylation	NDIGIGQSQDDSIVG CCCCCCCCCCCCCCC	30.29	29899451
579	Phosphorylation	IPAALPVSGTLSSSN CCCCCCCCCCCCCCC	24.91	25619855
581	Phosphorylation	AALPVSGTLSSSNPD CCCCCCCCCCCCCHH	18.89	25619855
583	Phosphorylation	LPVSGTLSSSNPDLL CCCCCCCCCCCHHHH	31.40	25619855
584	Phosphorylation	PVSGTLSSSNPDLLQ CCCCCCCCCCHHHHH	36.93	22324799
585	Phosphorylation	VSGTLSSSNPDLLQS CCCCCCCCCHHHHHH	49.35	27087446
592	Phosphorylation	SNPDLLQSHHRILDF CCHHHHHHCCEECCC	22.64	25619855
600	Phosphorylation	HHRILDFSTTPDLPD CCEECCCCCCCCCCH	30.91	29899451
601	Phosphorylation	HRILDFSTTPDLPDQ CEECCCCCCCCCCHH	42.20	20415495
602	Phosphorylation	RILDFSTTPDLPDQV EECCCCCCCCCCHHH	17.38	20415495
619	Phosphorylation	VFKADQQSRYIMISK HHEHHCCCCEEEEEC	22.78	-
621	Phosphorylation	KADQQSRYIMISKDT EHHCCCCEEEEECCC	10.49	-
644	Phosphorylation	AIREFAVTATPEQYS HHHHHCCCCCHHHCC	22.76	-
698	Phosphorylation	METETLCSDEDAQEL CCCCEECCHHHHHHH	47.34	29899451
806	Phosphorylation	RECKNFNSMFAIISG HHCCCHHHHHHHHHC	15.90	-
930	Phosphorylation	TRKKKWRSLGSLSQG CCCHHHHHHCCCCCC	35.65	25521595
933	Phosphorylation	KKWRSLGSLSQGSAN HHHHHHCCCCCCCCC	30.09	25521595
935	Phosphorylation	WRSLGSLSQGSANAT HHHHCCCCCCCCCCE	33.90	28066266
938	Phosphorylation	LGSLSQGSANATVLD HCCCCCCCCCCEEEH	15.65	28066266
942	Phosphorylation	SQGSANATVLDVAQT CCCCCCCEEEHHHHH	22.78	26804993
959	Phosphorylation	HKKRVRRSSFLNAKK CHHHHCHHHCCCHHH	18.19	25521595
960	Phosphorylation	KKRVRRSSFLNAKKL HHHHCHHHCCCHHHH	31.76	25521595
980	Phosphorylation	MARKVKQYLSNLELE HHHHHHHHHHHCCCC	13.33	22802335
982	Phosphorylation	RKVKQYLSNLELEMD HHHHHHHHHCCCCCC	34.14	22802335
992	Phosphorylation	ELEMDEESLQTLSLQ CCCCCHHHHHHHEEE	24.59	22802335
995	Phosphorylation	MDEESLQTLSLQCEP CCHHHHHHHEEEEEE	24.49	22802335
1019	Phosphorylation	GDKKPVKSETSPVAP CCCCCCCCCCCCCCC	46.47	25521595
1021	Phosphorylation	KKPVKSETSPVAPRA CCCCCCCCCCCCCCC	45.48	27742792
1022	Phosphorylation	KPVKSETSPVAPRAG CCCCCCCCCCCCCCC	17.21	25521595
1061	Phosphorylation	QVPAVSLYPSRKKVP CCCEEEECCCCCCCC	7.52	29514104
1079	Phosphorylation	LPPFGINSPQALKKI CCCCCCCCHHHHHHH	19.23	27087446
1088	Phosphorylation	QALKKILSLSEEGSL HHHHHHHCCCCCCCH	33.07	26239621
1090	Phosphorylation	LKKILSLSEEGSLER HHHHHCCCCCCCHHH	30.17	26824392
1094	Phosphorylation	LSLSEEGSLERHRKQ HCCCCCCCHHHHHHH	30.27	23527152
1105	Phosphorylation	HRKQAEDTISNASSQ HHHHHHHHHHHHHHH	20.15	26643407
1107	Phosphorylation	KQAEDTISNASSQLS HHHHHHHHHHHHHHC	28.56	23737553
1110	Phosphorylation	EDTISNASSQLSSPP HHHHHHHHHHHCCCC	23.91	27087446
1111	Phosphorylation	DTISNASSQLSSPPT HHHHHHHHHHCCCCC	32.50	27087446
1114	Phosphorylation	SNASSQLSSPPTSPQ HHHHHHHCCCCCCCC	32.91	25521595
1115	Phosphorylation	NASSQLSSPPTSPQS HHHHHHCCCCCCCCC	43.41	25521595
1118	Phosphorylation	SQLSSPPTSPQSSPR HHHCCCCCCCCCCCC	57.32	27087446
1119	Phosphorylation	QLSSPPTSPQSSPRK HHCCCCCCCCCCCCC	27.07	25521595
1122	Phosphorylation	SPPTSPQSSPRKGYA CCCCCCCCCCCCCEE	45.77	23737553
1123	Phosphorylation	PPTSPQSSPRKGYAL CCCCCCCCCCCCEEE	24.39	23737553
1128	Phosphorylation	QSSPRKGYALALSGT CCCCCCCEEEEEECE	11.07	29514104
1151	Phosphorylation	HSEISSRSSIVSNSS CCCCCCCCCCCCCCC	26.77	25338131
1152	Phosphorylation	SEISSRSSIVSNSSF CCCCCCCCCCCCCCC	26.37	26370283
1155	Phosphorylation	SSRSSIVSNSSFDSV CCCCCCCCCCCCCCC	29.35	26370283
1157	Phosphorylation	RSSIVSNSSFDSVPV CCCCCCCCCCCCCCC	25.52	29550500
1158	Phosphorylation	SSIVSNSSFDSVPVS CCCCCCCCCCCCCCC	37.01	30372032
1165	O-linked_Glycosylation	SFDSVPVSLHDERRQ CCCCCCCCCCHHHHH	17.57	22517741
1175	Phosphorylation	DERRQRHSVSIVESN HHHHHHCCEEEECCC	21.54	19060867
1177	Phosphorylation	RRQRHSVSIVESNLG HHHHCCEEEECCCCC	24.75	19060867
1200	Phosphorylation	LMEPDQYSLGSYAPV CCCCCCCCCCCCCCC	22.18	29514104
1221	Phosphorylation	YAAATVISSPSTEEL EEEEEEEECCCHHHH	31.68	29899451
1222	Phosphorylation	AAATVISSPSTEELS EEEEEEECCCHHHHC	16.25	29899451
1224	Phosphorylation	ATVISSPSTEELSHD EEEEECCCHHHHCCC	50.85	29899451
1225	Phosphorylation	TVISSPSTEELSHDQ EEEECCCHHHHCCCC	36.54	29899451
1229	Phosphorylation	SPSTEELSHDQGDRA CCCHHHHCCCCCCCH	28.44	29899451
1237	Phosphorylation	HDQGDRASLDAADSG CCCCCCHHCCHHHCC	27.89	29899451
1247	Phosphorylation	AADSGRGSWTSCSSG HHHCCCCCCCCCCCC	26.31	25777480
1249	Phosphorylation	DSGRGSWTSCSSGSH HCCCCCCCCCCCCCC	22.89	25777480
1250	Phosphorylation	SGRGSWTSCSSGSHD CCCCCCCCCCCCCCC	13.02	25777480
1252	Phosphorylation	RGSWTSCSSGSHDNI CCCCCCCCCCCCCCC	37.18	26643407
1253	Phosphorylation	GSWTSCSSGSHDNIQ CCCCCCCCCCCCCCC	48.95	26643407
1255	Phosphorylation	WTSCSSGSHDNIQTI CCCCCCCCCCCCCEE	29.61	26643407
1261	Phosphorylation	GSHDNIQTIQHQRSW CCCCCCCEEEECCCC	20.86	25367039
1312	Phosphorylation	KYNRQNQSRESLEQA HHHHCHHCHHHHHHH	45.63	29899451
1315	Phosphorylation	RQNQSRESLEQAQSR HCHHCHHHHHHHHHH	35.78	29899451
1324	Phosphorylation	EQAQSRASWASSTGY HHHHHHHHHHHHCCC	23.28	26643407
1327	Phosphorylation	QSRASWASSTGYWGE HHHHHHHHHCCCCCC	23.36	26643407
1328	Phosphorylation	SRASWASSTGYWGED HHHHHHHHCCCCCCC	20.36	25338131
1331	Phosphorylation	SWASSTGYWGEDSEG HHHHHCCCCCCCCCC	15.44	25338131
1336	Phosphorylation	TGYWGEDSEGDTGTI CCCCCCCCCCCCCCE	38.89	29899451
1340	Phosphorylation	GEDSEGDTGTIKRRG CCCCCCCCCCEEECC	47.27	-
1342	Phosphorylation	DSEGDTGTIKRRGGK CCCCCCCCEEECCCC	25.97	-
1364	O-linked_Glycosylation	SSSMVPVTTEEAKPV CCCCCCCCCCCCCCC	23.55	22517741
1365	O-linked_Glycosylation	SSMVPVTTEEAKPVP CCCCCCCCCCCCCCC	31.87	22517741
1401	Phosphorylation	RYREPPPTPPGYVGI CCCCCCCCCCCCCCC	48.14	21659605
1427	Phosphorylation	PARKPPDYNVALQRS CCCCCCCCCHHHHHC	19.81	29514104
1449	Phosphorylation	EAPAPGQTPPAAAAS CCCCCCCCCCHHHHC	36.84	28066266

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
644	T	Phosphorylation	Kinase	PLK2	P53351	Uniprot
806	S	Phosphorylation	Kinase	PLK2	P53351	Uniprot
933	S	Phosphorylation	Kinase	PLK2	P53351	Uniprot
1022	S	Phosphorylation	Kinase	PLK2	P53351	Uniprot
1175	S	Phosphorylation	Kinase	PLK2	P53351	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RPGF2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RPGF2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of RPGF2_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RPGF2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry."; Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.; J. Proteome Res. 6:250-262(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-418 AND SER-420, ANDMASS SPECTROMETRY.	17203969 [Abstract]