RN145_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RN145_HUMAN
• UniProt AC: Q96MT1
• Protein Name: RING finger protein 145 {ECO:0000305}
• Gene Name: RNF145 {ECO:0000312|PROSITE:PS50089}
• Organism: Homo sapiens (Human).
• Sequence Length: 663
• Subcellular Localization: Endoplasmic reticulum membrane ; Multi-pass membrane protein .
• Protein Description: E3 ubiquitin ligase that catalyzes the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. In response to bacterial infection, negatively regulates the phagocyte oxidative burst by controlling the turnover of the NADPH oxidase complex subunits. Promotes monoubiquitination of CYBA and 'Lys-48'-linked polyubiquitination and degradation of CYBB NADPH oxidase catalytic subunits, both essential for the generation of antimicrobial reactive oxygen species. Involved in the maintenance of cholesterol homeostasis. In response to high sterol concentrations ubiquitinates HMGCR, a rate-limiting enzyme in cholesterol biosynthesis, and targets it for degradation. The interaction with INSIG1 is required for this function. In addition, triggers ubiquitination of SCAP, likely inhibiting its transport to the Golgi apparatus and the subsequent processing/maturation of SREBPF2, ultimately downregulating cholesterol biosynthesis..
• Protein Sequence: MAAKEKLEAVLNVALRVPSIMLLDVLYRWDVSSFFQQIQRSSLSNNPLFQYKYLALNMHYVGYILSVVLLTLPRQHLVQLYLYFLTALLLYAGHQISRDYVRSELEFAYEGPMYLEPLSMNRFTTALIGQLVVCTLCSCVMKTKQIWLFSAHMLPLLARLCLVPLETIVIINKFAMIFTGLEVLYFLGSNLLVPYNLAKSAYRELVQVVEVYGLLALGMSLWNQLVVPVLFMVFWLVLFALQIYSYFSTRDQPASRERLLFLFLTSIAECCSTPYSLLGLVFTVSFVALGVLTLCKFYLQGYRAFMNDPAMNRGMTEGVTLLILAVQTGLIELQVVHRAFLLSIILFIVVASILQSMLEIADPIVLALGASRDKSLWKHFRAVSLCLFLLVFPAYMAYMICQFFHMDFWLLIIISSSILTSLQVLGTLFIYVLFMVEEFRKEPVENMDDVIYYVNGTYRLLEFLVALCVVAYGVSETIFGEWTVMGSMIIFIHSYYNVWLRAQLGWKSFLLRRDAVNKIKSLPIATKEQLEKHNDICAICYQDMKSAVITPCSHFFHAGCLKKWLYVQETCPLCHCHLKNSSQLPGLGTEPVLQPHAGAEQNVMFQEGTEPPGQEHTPGTRIQEGSRDNNEYIARRPDNQEGAFDPKEYPHSAKDEAHPVESA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9 (in isoform 2)	Phosphorylation	-	22.87	22210691
9 (in isoform 5)	Phosphorylation	-	22.87	30631047
11 (in isoform 5)	Phosphorylation	-	5.71	30631047
13 (in isoform 5)	Phosphorylation	-	4.67	30631047
15 (in isoform 5)	Phosphorylation	-	5.03	30631047
18	Phosphorylation	LNVALRVPSIMLLDV HHHHHHCCHHHHHHH	16.23	-
18 (in isoform 2)	Phosphorylation	-	16.23	-
19	Phosphorylation	NVALRVPSIMLLDVL HHHHHCCHHHHHHHH	20.13	27732954
20 (in isoform 5)	Phosphorylation	-	1.52	30631047
53	Phosphorylation	NPLFQYKYLALNMHY CCCHHHHHHHHHHHH	7.71	24043423
60	Phosphorylation	YLALNMHYVGYILSV HHHHHHHHHHHHHHH	5.70	24043423
63	Phosphorylation	LNMHYVGYILSVVLL HHHHHHHHHHHHHHH	6.66	24043423
66	Phosphorylation	HYVGYILSVVLLTLP HHHHHHHHHHHHHCC	10.71	24043423
71	Phosphorylation	ILSVVLLTLPRQHLV HHHHHHHHCCHHHHH	30.47	24719451
298	Phosphorylation	VLTLCKFYLQGYRAF HHHHHHHHHHHHHHH	5.43	-
352	Phosphorylation	ILFIVVASILQSMLE HHHHHHHHHHHHHHH	16.54	-
507 (in isoform 1)	Ubiquitination	-	29.54	21890473
507	Ubiquitination	LRAQLGWKSFLLRRD HHHHHCCCHHHHCHH	29.54	-
508	Phosphorylation	RAQLGWKSFLLRRDA HHHHCCCHHHHCHHH	18.02	24719451
520	Ubiquitination	RDAVNKIKSLPIATK HHHHHHHHCCCCCCH	47.77	-
520 (in isoform 1)	Ubiquitination	-	47.77	21890473
521	Phosphorylation	DAVNKIKSLPIATKE HHHHHHHCCCCCCHH	42.74	-
527 (in isoform 1)	Ubiquitination	-	34.15	21890473
527	Ubiquitination	KSLPIATKEQLEKHN HCCCCCCHHHHHHCC	34.15	-
532	Ubiquitination	ATKEQLEKHNDICAI CCHHHHHHCCCEEEE	57.19	-
535 (in isoform 2)	Ubiquitination	-	36.74	21890473
535	Ubiquitination	EQLEKHNDICAICYQ HHHHHCCCEEEEHHH	36.74	21890473
535	Ubiquitination	EQLEKHNDICAICYQ HHHHHCCCEEEEHHH	36.74	21890473
535	Ubiquitination	EQLEKHNDICAICYQ HHHHHCCCEEEEHHH	36.74	21890473
545	Ubiquitination	AICYQDMKSAVITPC EEHHHCHHHCEEECC	43.48	-
548 (in isoform 2)	Ubiquitination	-	6.90	21890473
555 (in isoform 2)	Ubiquitination	-	2.49	21890473
562	Ubiquitination	FFHAGCLKKWLYVQE HCCCCHHHHHHHHCC	46.08	-
563	Ubiquitination	FHAGCLKKWLYVQET CCCCHHHHHHHHCCC	29.50	-
647	Ubiquitination	QEGAFDPKEYPHSAK CCCCCCCCCCCCCCC	72.26	-
662 (in isoform 5)	Phosphorylation	-	39.24	27642862

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RN145_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RN145_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RN145_HUMAN !!

Protein-Protein Interaction

Oops, there are no PPI records of RN145_HUMAN !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.