dbPTM

RIC8A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RIC8A_MOUSE
UniProt AC	Q3TIR3
Protein Name	Synembryn-A
Gene Name	Ric8a
Organism	Mus musculus (Mouse).
Sequence Length	530
Subcellular Localization	Cytoplasm. Cell membrane. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity). Colocalizes with RIC8A in CA2 hippocampal neurons.
Protein Description	Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity)..
Protein Sequence	MEPRAVADALETGEEDAVTEALRSFNREHSQSFTFDDAQQEDRKRLAKLLVSVLEQGLSPKHRVTWLQTIRILSRDRSCLDSFASRQSLHALACYADITVSEEPIPQSPDMDVLLESLKCLCNLVLSSPTAQMLAAEARLVVRLAERVGLYRKRSYPHEVQFFDLRLLFLLTALRTDVRQQLFQELHGVRLLTDALELTLGVAPKENPPVMLPAQETERAMEILKVLFNITFDSVKREVDEEDAALYRYLGTLLRHCVMVEAAGDRTEEFHGHTVNLLGNLPLKCLDVLLALELHEGSLEFMGVNMDVISALLAFLEKRLHQTHRLKECVAPVLNVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGDLLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTEEYREAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVNMFDKLSRHRVIQPMGMSPRGHLTSLQDAMCETMEGQLSSDPDSDPD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
59	Phosphorylation	SVLEQGLSPKHRVTW HHHHCCCCHHHHCHH	38.43	22324799
122	S-palmitoylation	LESLKCLCNLVLSSP HHHHHHHHHHHHCCH	5.33	28680068
155	Phosphorylation	VGLYRKRSYPHEVQF HCCCCCCCCCCCCCH	46.16	28507225
199	Phosphorylation	LTDALELTLGVAPKE HHHHHHHHHCCCCCC	16.46	17203969
410	Phosphorylation	VPRFIKYTGYGNAAG CCCHHHHCCCCCHHH	20.94	22817900
412	Phosphorylation	RFIKYTGYGNAAGLL CHHHHCCCCCHHHHH	10.21	22817900
434	Phosphorylation	GGRPEGQYSEDEDTD CCCCCCCCCCCCCCC	25.53	25521595
435	Phosphorylation	GRPEGQYSEDEDTDT CCCCCCCCCCCCCCH	30.59	27087446
440	Phosphorylation	QYSEDEDTDTEEYRE CCCCCCCCCHHHHHH	42.65	27087446
442	Phosphorylation	SEDEDTDTEEYREAK CCCCCCCHHHHHHHH	32.58	21082442
445	Phosphorylation	EDTDTEEYREAKASI CCCCHHHHHHHHHHC	13.92	22802335
451	Phosphorylation	EYREAKASINPVTGR HHHHHHHHCCCCCCC	23.38	20139300
456	Phosphorylation	KASINPVTGRVEEKP HHHCCCCCCCCCCCC	22.11	20139300
488	Ubiquitination	KLVNMFDKLSRHRVI HHHHHHHHHCCCCCC	36.78	22790023
501	Phosphorylation	VIQPMGMSPRGHLTS CCCCCCCCCCCCCHH	13.01	26824392
516	Phosphorylation	LQDAMCETMEGQLSS HHHHHHHHHCCCCCC	18.65	21743459
522	Phosphorylation	ETMEGQLSSDPDSDP HHHCCCCCCCCCCCC	25.47	27087446
523	Phosphorylation	TMEGQLSSDPDSDPD HHCCCCCCCCCCCCC	62.85	27087446
527	Phosphorylation	QLSSDPDSDPD---- CCCCCCCCCCC----	57.33	27087446

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
501	S	Phosphorylation	Kinase	PRKCI	Q62074	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RIC8A_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RIC8A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of RIC8A_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RIC8A_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-435, AND MASSSPECTROMETRY.	17242355 [Abstract]
"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry."; Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.; J. Proteome Res. 6:250-262(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-199, AND MASSSPECTROMETRY.	17203969 [Abstract]