RHOH_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RHOH_HUMAN
• UniProt AC: Q15669
• Protein Name: Rho-related GTP-binding protein RhoH
• Gene Name: RHOH
• Organism: Homo sapiens (Human).
• Sequence Length: 191
• Subcellular Localization: Cytoplasm . Cell membrane; Lipid-anchor; Cytoplasmic side. Colocalizes together with ZAP70 in the immunological synapse..
• Protein Description: Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils..
• Protein Sequence: MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWIGEIRSNLPCTPVLVVATQTDQREMGPHRASCVNAMEGKKLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRRLFSINECKIF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MLSSIKCVLV -----CCCCCEEEEE	28.81	24719451
6	Ubiquitination	--MLSSIKCVLVGDS --CCCCCEEEEECCC	21.90	-
13	Phosphorylation	KCVLVGDSAVGKTSL EEEEECCCCCCCEEE	20.94	-
17	Ubiquitination	VGDSAVGKTSLLVRF ECCCCCCCEEEEEEE	28.48	-
53	Phosphorylation	FMDGIQISLGLWDTA EECCEEEEEECCCCC	10.46	22210691
67	Phosphorylation	AGNDAFRSIRPLSYQ CCCHHHHHCCCCCHH	19.25	22210691
72	Phosphorylation	FRSIRPLSYQQADVV HHHCCCCCHHCCCEE	24.47	23663014
73	Phosphorylation	RSIRPLSYQQADVVL HHCCCCCHHCCCEEE	16.60	23663014
83	Phosphorylation	ADVVLMCYSVANHNS CCEEEEEEECCCCCH	7.69	23663014
84	Phosphorylation	DVVLMCYSVANHNSF CEEEEEEECCCCCHH	15.15	23663014
90	Phosphorylation	YSVANHNSFLNLKNK EECCCCCHHHHCCCC	24.45	23663014
97	Ubiquitination	SFLNLKNKWIGEIRS HHHHCCCCHHHHHHH	38.30	-
137	Ubiquitination	CVNAMEGKKLAQDVR HHHHHCHHHHHHHHH	31.16	-
138	Ubiquitination	VNAMEGKKLAQDVRA HHHHCHHHHHHHHHH	60.97	-
146	Ubiquitination	LAQDVRAKGYLECSA HHHHHHHCCCEEHHH	37.21	-
188	Methylation	RLFSINECKIF---- HCEECCCCCCC----	3.37	-
188	Geranylgeranylation	RLFSINECKIF---- HCEECCCCCCC----	3.37	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RHOH_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RHOH_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RHOH_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LRIF1_HUMAN	LRIF1	physical	16169070
UT14A_HUMAN	UTP14A	physical	16169070
GDIR1_HUMAN	ARHGDIA	physical	11809807
GDIR2_HUMAN	ARHGDIB	physical	11809807
GDIR3_HUMAN	ARHGDIG	physical	11809807

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.