dbPTM

RHG31_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RHG31_MOUSE
UniProt AC	A6X8Z5
Protein Name	Rho GTPase-activating protein 31
Gene Name	Arhgap31
Organism	Mus musculus (Mouse).
Sequence Length	1425
Subcellular Localization	Cell projection, lamellipodium. Cell junction, focal adhesion.
Protein Description	Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration..
Protein Sequence	MKNKGAKQKLKRKGAASAFGCDLTEYLESSGQDVPYVLKSCAEFIETHGIVDGIYRLSGITSNIQRLRQEFGSDQCPDLTREVYLQDIHCVGSLCKLYFRELPNPLLTYELYEKFTEAVSHRPEEGQLARIQNVILELPPPHYRTLEYLIRHLAHIASFSSKTNMHARNLALVWAPNLLRSKKIEATICNGDAAFLAVRVQQVVIEFILNHADQIFNGGAPGALQQDESRTITKSLTLPALSLPMKLVSLEEAQARSLATNHPARKERRENSLPEIVPPPFHTVLELPDNKRKLSSKSKKWKSIFNLGRSGSDSKSKLSRNGSVFVRGQRLSVEKATIRPAKSMDSLCSVPVEGKENKGNFSRTVTTGGFFIPATKMHASSTGSSCDLSKEGEWGQEGMPAGAEGGCEVGGQIRPLPEQLKVFRPIGDPESEQSAPKLLGMFYTSSDSPGKSVFTSSLFQMEPSPRHQRKALNISEPFAVSVPLRVSAVISTNSTPCRTPPKELQSLSSLEEFSFQGSESGGWPEEEKPLGAESFPGSVTKKAATEDTKPEPEVPGRAECSQSPPLDPGTQVEKKTLHVSLGSQVSKEAEKRPKAEKVMEESQGASQPKPSTPQESLGAGTEPLILHEMDEEDLAQALIWPEIQQELKIIESEEEFSSLPPAAQKTSPIPESSPAPFPFPEAPGSLPSSSAPREVWTRDAANQSIQEAAILTDREKLEPVCSLLESESQQELSPDPASLAPLEMLLFEKVSSPARIEIGGPRNLSPPLTPAPPPPTPLEEEPEVLLSKEGPDREDAARDSRTDVYTEQPTPKESPGIPTPCQREEAIASPNEKQNARHAVPENKGPGLPSPTKEVDIIPQEEGGAPHSAQEPSDCDEDDTVTDPAQHGLEMVEPWEEPQWVTSPLHSPTLKEVQESQTQGSQGHRLERRLCHRPSLRQSHSLDSKTTGNSHWTLEAPFSSSCANLETERNYEPLQPPAARTKIAGLEEKALKAFREFSGLKGLEVLPSQKGPSGIQPKPVETNFMGLAEGKEQEPQLELSNRQMKHSDVPGPDSSKESSPRAQDSTLPGEHPLQLQLKNTECGPSKGKHRPSSLNLDSATPIADLFRLENGAPFSSPGIELSELGDTKVTWMSSSHCKAAPWNSQDTQDLDIVAHTLTGRRNSAPVSVSAVRTSFMVKMCQAKAVPVIPPKIQYTQIPQPLPSQSTGEGGAQPLERSQEEPGSTPEIPQKSTKDDSPSSLGSPEEEQPKQETGASASRRQASITSCMYEGSSCSPEPSASTLASTQDAVVQCRKRTSETEPSGDNLLSSKLERASGGPKAFHRSRPGRPQSLILFPIMDHLPSSPTVIDSKVLLSPIRSPTQTVSPGLLCGELAENTWITPEGVTLRNKMTIPKNGQRLETSTSCFYQPQRRSVILDGRSGRQIE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
235	Phosphorylation	ESRTITKSLTLPALS HHCCCCCCCCHHHHC	20.24	29472430
237	Phosphorylation	RTITKSLTLPALSLP CCCCCCCCHHHHCCC	37.47	27180971
242	Phosphorylation	SLTLPALSLPMKLVS CCCHHHHCCCEEEEE	32.31	29514104
272	Phosphorylation	RKERRENSLPEIVPP HHHHHHCCCCCCCCC	40.31	25521595
283	Phosphorylation	IVPPPFHTVLELPDN CCCCCCCEEEECCCC	27.45	22817900
303	Phosphorylation	SKSKKWKSIFNLGRS CCCHHHHHHHCCCCC	31.15	25521595
323	Phosphorylation	SKLSRNGSVFVRGQR CHHCCCCCEEECCEE	18.87	24453211
332	Phosphorylation	FVRGQRLSVEKATIR EECCEECEEEEEEEC	30.35	26824392
337	Phosphorylation	RLSVEKATIRPAKSM ECEEEEEEECCCCCC	28.69	25619855
343	Phosphorylation	ATIRPAKSMDSLCSV EEECCCCCCCCCCCC	29.90	25521595
344	Oxidation	TIRPAKSMDSLCSVP EECCCCCCCCCCCCE	3.90	17242355
346	Phosphorylation	RPAKSMDSLCSVPVE CCCCCCCCCCCCEEC	23.72	25521595
349	Phosphorylation	KSMDSLCSVPVEGKE CCCCCCCCCEECCCC	34.45	23984901
366	Phosphorylation	GNFSRTVTTGGFFIP CCCCEEEEECCEEEE	21.07	22942356
380	Phosphorylation	PATKMHASSTGSSCD ECEEECCCCCCCCCC	17.47	27742792
381	Phosphorylation	ATKMHASSTGSSCDL CEEECCCCCCCCCCC	37.26	27742792
382	Phosphorylation	TKMHASSTGSSCDLS EEECCCCCCCCCCCC	38.29	27742792
384	Phosphorylation	MHASSTGSSCDLSKE ECCCCCCCCCCCCCC	28.07	26824392
385	Phosphorylation	HASSTGSSCDLSKEG CCCCCCCCCCCCCCC	17.00	20139300
389	Phosphorylation	TGSSCDLSKEGEWGQ CCCCCCCCCCCCCCC	18.53	27742792
446	Phosphorylation	LGMFYTSSDSPGKSV HCEEECCCCCCCCEE	34.02	26643407
448	Phosphorylation	MFYTSSDSPGKSVFT EEECCCCCCCCEEEC	37.18	26643407
464	Phosphorylation	SLFQMEPSPRHQRKA HHHCCCCCHHHHHHC	22.66	26824392
487	Phosphorylation	VSVPLRVSAVISTNS EECCEEEEEEEECCC	15.47	25521595
491	Phosphorylation	LRVSAVISTNSTPCR EEEEEEEECCCCCCC	18.22	26643407
492	Phosphorylation	RVSAVISTNSTPCRT EEEEEEECCCCCCCC	23.28	26643407
494	Phosphorylation	SAVISTNSTPCRTPP EEEEECCCCCCCCCC	33.54	25521595
495	Phosphorylation	AVISTNSTPCRTPPK EEEECCCCCCCCCCH	28.60	26643407
499	Phosphorylation	TNSTPCRTPPKELQS CCCCCCCCCCHHHHC	51.14	26643407
561	Phosphorylation	VPGRAECSQSPPLDP CCCCCCCCCCCCCCC	25.79	30635358
563	Phosphorylation	GRAECSQSPPLDPGT CCCCCCCCCCCCCCC	16.52	27149854
570	Phosphorylation	SPPLDPGTQVEKKTL CCCCCCCCCEEEEEE	34.53	29233185
580	Phosphorylation	EKKTLHVSLGSQVSK EEEEEEEECCCHHCH	19.10	30635358
583	Phosphorylation	TLHVSLGSQVSKEAE EEEEECCCHHCHHHH	32.47	30635358
586	Phosphorylation	VSLGSQVSKEAEKRP EECCCHHCHHHHHCH	19.69	19367708
652	Phosphorylation	QELKIIESEEEFSSL HHHHHHHCHHHHHCC	39.64	27180971
657	Phosphorylation	IESEEEFSSLPPAAQ HHCHHHHHCCCCHHC	33.96	30635358
658	Phosphorylation	ESEEEFSSLPPAAQK HCHHHHHCCCCHHCC	50.66	24453211
666	Phosphorylation	LPPAAQKTSPIPESS CCCHHCCCCCCCCCC	27.88	24723360
667	Phosphorylation	PPAAQKTSPIPESSP CCHHCCCCCCCCCCC	28.06	26824392
672	Phosphorylation	KTSPIPESSPAPFPF CCCCCCCCCCCCCCC	35.95	25619855
673	Phosphorylation	TSPIPESSPAPFPFP CCCCCCCCCCCCCCC	24.34	25619855
685	Phosphorylation	PFPEAPGSLPSSSAP CCCCCCCCCCCCCCC	35.73	25619855
688	Phosphorylation	EAPGSLPSSSAPREV CCCCCCCCCCCCCCH	42.07	25619855
689	Phosphorylation	APGSLPSSSAPREVW CCCCCCCCCCCCCHH	28.69	25619855
690	Phosphorylation	PGSLPSSSAPREVWT CCCCCCCCCCCCHHC	46.78	25619855
751	Phosphorylation	MLLFEKVSSPARIEI HHHHCCCCCCCEEEE	41.49	29550500
752	Phosphorylation	LLFEKVSSPARIEIG HHHCCCCCCCEEEEC	26.10	29550500
765	Phosphorylation	IGGPRNLSPPLTPAP ECCCCCCCCCCCCCC	28.20	24925903
769	Phosphorylation	RNLSPPLTPAPPPPT CCCCCCCCCCCCCCC	24.15	24925903
776	Phosphorylation	TPAPPPPTPLEEEPE CCCCCCCCCCCCCCC	46.40	25619855
800	Phosphorylation	REDAARDSRTDVYTE HHHHHHHCCCCCCCC	31.37	25338131
814	Phosphorylation	EQPTPKESPGIPTPC CCCCCCCCCCCCCCC	34.25	25338131
829	Phosphorylation	QREEAIASPNEKQNA CCHHHCCCCCHHHHH	23.34	28285833
850	Phosphorylation	NKGPGLPSPTKEVDI CCCCCCCCCCCEEEE	50.69	24925903
852	Phosphorylation	GPGLPSPTKEVDIIP CCCCCCCCCEEEECC	44.14	24925903
935	Phosphorylation	RRLCHRPSLRQSHSL HHHHCCCCCCCCCCC	35.82	26824392
939	Phosphorylation	HRPSLRQSHSLDSKT CCCCCCCCCCCCCCC	14.49	23737553
941	Phosphorylation	PSLRQSHSLDSKTTG CCCCCCCCCCCCCCC	38.83	30352176
944	Phosphorylation	RQSHSLDSKTTGNSH CCCCCCCCCCCCCCC	36.88	30635358
959	Phosphorylation	WTLEAPFSSSCANLE EEEECCCCCCCCCCC	21.93	23984901
960	Phosphorylation	TLEAPFSSSCANLET EEECCCCCCCCCCCC	29.80	19060867
961	Phosphorylation	LEAPFSSSCANLETE EECCCCCCCCCCCCC	18.95	22817900
1054	Phosphorylation	SDVPGPDSSKESSPR CCCCCCCCCCCCCCC	46.58	29550500
1055	Phosphorylation	DVPGPDSSKESSPRA CCCCCCCCCCCCCCC	48.07	29550500
1058	Phosphorylation	GPDSSKESSPRAQDS CCCCCCCCCCCCCCC	49.75	30635358
1059	Phosphorylation	PDSSKESSPRAQDST CCCCCCCCCCCCCCC	21.35	30635358
1092	Phosphorylation	SKGKHRPSSLNLDSA CCCCCCCCCCCCCCC	47.62	25521595
1093	Phosphorylation	KGKHRPSSLNLDSAT CCCCCCCCCCCCCCC	24.78	25521595
1098	Phosphorylation	PSSLNLDSATPIADL CCCCCCCCCCCHHHH	36.89	19060867
1100	Phosphorylation	SLNLDSATPIADLFR CCCCCCCCCHHHHHH	21.12	25367039
1115	Phosphorylation	LENGAPFSSPGIELS CCCCCCCCCCCEEHH	34.27	24719451
1116	Phosphorylation	ENGAPFSSPGIELSE CCCCCCCCCCEEHHH	27.76	26824392
1144	Phosphorylation	CKAAPWNSQDTQDLD CCCCCCCCCCCCCCC	26.09	24719451
1147	Phosphorylation	APWNSQDTQDLDIVA CCCCCCCCCCCCEEE	19.15	23567750
1163	Phosphorylation	TLTGRRNSAPVSVSA HHCCCCCCCCCCHHH	31.94	25521595
1167	Phosphorylation	RRNSAPVSVSAVRTS CCCCCCCCHHHHHHH	14.86	25619855
1169	Phosphorylation	NSAPVSVSAVRTSFM CCCCCCHHHHHHHHH	17.34	23684622
1206	Phosphorylation	QPLPSQSTGEGGAQP CCCCCCCCCCCCCCC	31.45	25338131
1223	Phosphorylation	RSQEEPGSTPEIPQK CCCCCCCCCCCCCCC	52.05	30635358
1224	Phosphorylation	SQEEPGSTPEIPQKS CCCCCCCCCCCCCCC	30.75	30635358
1231	Phosphorylation	TPEIPQKSTKDDSPS CCCCCCCCCCCCCCH	35.13	30635358
1232	Phosphorylation	PEIPQKSTKDDSPSS CCCCCCCCCCCCCHH	45.59	30635358
1236	Phosphorylation	QKSTKDDSPSSLGSP CCCCCCCCCHHCCCC	36.49	25521595
1238	Phosphorylation	STKDDSPSSLGSPEE CCCCCCCHHCCCCCH	41.99	27742792
1239	Phosphorylation	TKDDSPSSLGSPEEE CCCCCCHHCCCCCHH	39.88	27742792
1242	Phosphorylation	DSPSSLGSPEEEQPK CCCHHCCCCCHHCCC	34.14	25521595
1252	Phosphorylation	EEQPKQETGASASRR HHCCCCCCCCCHHHH	35.24	27742792
1255	Phosphorylation	PKQETGASASRRQAS CCCCCCCCHHHHHHE	28.28	27742792
1257	Phosphorylation	QETGASASRRQASIT CCCCCCHHHHHHEEE	26.04	27742792
1296	Phosphorylation	VVQCRKRTSETEPSG HHHHHHCCCCCCCCC	33.67	29550500
1297	Phosphorylation	VQCRKRTSETEPSGD HHHHHCCCCCCCCCC	46.70	29550500
1299	Phosphorylation	CRKRTSETEPSGDNL HHHCCCCCCCCCCCC	53.62	29550500
1324	Phosphorylation	GPKAFHRSRPGRPQS CCCCCCCCCCCCCCE	33.97	26160508
1331	Phosphorylation	SRPGRPQSLILFPIM CCCCCCCEEEEEEEH	21.76	26824392
1344	Phosphorylation	IMDHLPSSPTVIDSK EHHCCCCCCEEECCE	23.73	29514104
1355	Phosphorylation	IDSKVLLSPIRSPTQ ECCEEECCCCCCCCC	17.70	27180971
1359	Phosphorylation	VLLSPIRSPTQTVSP EECCCCCCCCCCCCC	32.78	26643407
1361	Phosphorylation	LSPIRSPTQTVSPGL CCCCCCCCCCCCCCC	37.81	26643407
1363	Phosphorylation	PIRSPTQTVSPGLLC CCCCCCCCCCCCCCC	25.98	23649490
1365	Phosphorylation	RSPTQTVSPGLLCGE CCCCCCCCCCCCCHH	19.35	23649490
1407	Phosphorylation	ETSTSCFYQPQRRSV EECCCCCCCCCCCEE	24.50	29514104
1413	Phosphorylation	FYQPQRRSVILDGRS CCCCCCCEEEEECCC	18.72	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
769	T	Phosphorylation	Kinase	MAPK3	P27361	GPS
776	T	Phosphorylation	Kinase	GSK3A	P49840	PSP
776	T	Phosphorylation	Kinase	GSK3B	P49841	PSP
776	T	Phosphorylation	Kinase	MAPK3	P27361	GPS
776	T	Phosphorylation	Kinase	GSK-FAMILY	-	GPS
776	T	Phosphorylation	Kinase	GSK3	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
776	T	Phosphorylation		16024771
Phosphorylated on Thr-776 by GSK3; which reduces GAP activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RHG31_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
ITSN1_MOUSE	Itsn1	physical	11744688

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RHG31_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1163, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1092 AND SER-1093, ANDMASS SPECTROMETRY.	17242355 [Abstract]
"Glycogen synthase kinase-3 phosphorylates CdGAP at a consensus ERK 1regulatory site."; Danek E.I., Tcherkezian J., Triki I., Meriane M., Lamarche-Vane N.; J. Biol. Chem. 282:3624-3631(2007). Cited for: PHOSPHORYLATION AT THR-776, AND INDUCTION BY SERUM.	17158447 [Abstract]
"Extracellular signal-regulated kinase 1 interacts with andphosphorylates CdGAP at an important regulatory site."; Tcherkezian J., Danek E.I., Jenna S., Triki I., Lamarche-Vane N.; Mol. Cell. Biol. 25:6314-6329(2005). Cited for: IDENTIFICATION, AND PHOSPHORYLATION AT THR-776.	16024771 [Abstract]

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